{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,7]],"date-time":"2026-07-07T23:44:58Z","timestamp":1783467898849,"version":"3.55.0"},"reference-count":70,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,4,6]],"date-time":"2017-04-06T00:00:00Z","timestamp":1491436800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,4,6]],"date-time":"2017-04-06T00:00:00Z","timestamp":1491436800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Commun"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In response to infection and injury, the neutrophil population rapidly expands and then quickly re-establishes the basal state when inflammation resolves. The exact pathways governing neutrophil\/macrophage lineage outputs from a common granulocyte-macrophage progenitor are still not completely understood. From a forward genetic screen in zebrafish, we identify the transcriptional repressor, ZBTB11, as critical for basal and emergency granulopoiesis. ZBTB11 sits in a pathway directly downstream of master myeloid regulators including PU.1, and<jats:italic>TP53<\/jats:italic>is one direct ZBTB11 transcriptional target.<jats:italic>TP53<\/jats:italic>repression is dependent on ZBTB11 cys116, which is a functionally critical, metal ion-coordinating residue within a novel viral integrase-like zinc finger domain. To our knowledge, this is the first description of a function for this domain in a cellular protein. We demonstrate that the PU.1\u2013ZBTB11\u2013TP53 pathway is conserved from fish to mammals. Finally, Zbtb11 mutant rescue experiments point to a ZBTB11-regulated TP53 requirement in development of other organs.<\/jats:p>","DOI":"10.1038\/ncomms14911","type":"journal-article","created":{"date-parts":[[2017,4,6]],"date-time":"2017-04-06T10:57:13Z","timestamp":1491476233000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":34,"title":["The Pu.1 target gene Zbtb11 regulates neutrophil development through its integrase-like HHCC zinc finger"],"prefix":"10.1038","volume":"8","author":[{"given":"Maria-Cristina","family":"Keightley","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duncan P.","family":"Carradice","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith E.","family":"Layton","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luke","family":"Pase","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julien Y.","family":"Bertrand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0598-2897","authenticated-orcid":false,"given":"Johannes G.","family":"Wittig","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandar","family":"Dakic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew P.","family":"Badrock","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicholas J.","family":"Cole","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Traver","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen L.","family":"Nutt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"McCoey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2943-9044","authenticated-orcid":false,"given":"Ashley M.","family":"Buckle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joan K.","family":"Heath","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0325-798X","authenticated-orcid":false,"given":"Graham J.","family":"Lieschke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,4,6]]},"reference":[{"key":"BFncomms14911_CR1","doi-asserted-by":"publisher","first-page":"705","DOI":"10.1172\/JCI108517","volume":"58","author":"JT Dancey","year":"1976","unstructured":"Dancey, J. T., Deubelbeiss, K. A., Harker, L. A. & Finch, C. A. Neutrophil kinetics in man. J. Clin. Invest. 58, 705\u2013715 (1976).","journal-title":"J. Clin. Invest."},{"key":"BFncomms14911_CR2","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1016\/j.cell.2008.01.025","volume":"132","author":"SH Orkin","year":"2008","unstructured":"Orkin, S. H. & Zon, L. I. Hematopoiesis: an evolving paradigm for stem cell biology. Cell 132, 631\u2013644 (2008).","journal-title":"Cell"},{"key":"BFncomms14911_CR3","doi-asserted-by":"publisher","first-page":"105","DOI":"10.1038\/nri2024","volume":"7","author":"F Rosenbauer","year":"2007","unstructured":"Rosenbauer, F. & Tenen, D. G. Transcription factors in myeloid development: balancing differentiation with transformation. Nat. Rev. Immunol. 7, 105\u2013117 (2007).","journal-title":"Nat. Rev. Immunol."},{"key":"BFncomms14911_CR4","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1038\/35004599","volume":"404","author":"K Akashi","year":"2000","unstructured":"Akashi, K., Traver, D., Miyamoto, T. & Weissman, I. L. A clonogenic common myeloid progenitor that gives rise to all myeloid lineages. Nature 404, 193\u2013197 (2000).","journal-title":"Nature"},{"key":"BFncomms14911_CR5","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1038\/nri3660","volume":"14","author":"MG Manz","year":"2014","unstructured":"Manz, M. G. & Boettcher, S. Emergency granulopoiesis. Nat. Rev. Immunol. 14, 302\u2013314 (2014).","journal-title":"Nat. Rev. Immunol."},{"key":"BFncomms14911_CR6","doi-asserted-by":"publisher","first-page":"1237","DOI":"10.1002\/stem.1379","volume":"31","author":"L Wolff","year":"2013","unstructured":"Wolff, L. & Humeniuk, R. Concise review: erythroid versus myeloid lineage commitment: regulating the master regulators. Stem Cells 31, 1237\u20131244 (2013).","journal-title":"Stem Cells"},{"key":"BFncomms14911_CR7","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1016\/j.stem.2008.11.006","volume":"4","author":"Y Liu","year":"2009","unstructured":"Liu, Y. et al. p53 regulates hematopoietic stem cell quiescence. Cell Stem Cell 4, 37\u201348 (2009).","journal-title":"Cell Stem Cell"},{"key":"BFncomms14911_CR8","doi-asserted-by":"publisher","first-page":"5118","DOI":"10.1182\/blood-2012-05-356014","volume":"120","author":"V Pant","year":"2012","unstructured":"Pant, V., Quintas-Cardama, A. & Lozano, G. The p53 pathway in hematopoiesis: lessons from mouse models, implications for humans. Blood 120, 5118\u20135127 (2012).","journal-title":"Blood"},{"key":"BFncomms14911_CR9","doi-asserted-by":"publisher","first-page":"462","DOI":"10.1128\/MCB.23.2.462-473.2003","volume":"23","author":"SM Mendrysa","year":"2003","unstructured":"Mendrysa, S. M. et al. mdm2 is critical for inhibition of p53 during lymphopoiesis and the response to ionizing irradiation. Mol. Cell Biol. 23, 462\u2013473 (2003).","journal-title":"Mol. Cell Biol."},{"key":"BFncomms14911_CR10","doi-asserted-by":"publisher","first-page":"3358","DOI":"10.4161\/cc.21277","volume":"11","author":"OM Siggs","year":"2012","unstructured":"Siggs, O. M. & Beutler, B. The BTB-ZF transcription factors. Cell Cycle 11, 3358\u20133369 (2012).","journal-title":"Cell Cycle"},{"key":"BFncomms14911_CR11","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1038\/icb.2014.20","volume":"92","author":"S Chevrier","year":"2014","unstructured":"Chevrier, S. & Corcoran, L. M. BTB-ZF transcription factors, a growing family of regulators of early and late B-cell development. Immunol. Cell Biol. 92, 481\u2013488 (2014).","journal-title":"Immunol. Cell Biol."},{"key":"BFncomms14911_CR12","doi-asserted-by":"publisher","first-page":"2841","DOI":"10.4049\/jimmunol.1004006","volume":"187","author":"AM Beaulieu","year":"2011","unstructured":"Beaulieu, A. M. & Sant\u2019Angelo, D. B. The BTB-ZF family of transcription factors: key regulators of lineage commitment and effector function development in the immune system. J. Immunol. 187, 2841\u20132847 (2011).","journal-title":"J. Immunol."},{"key":"BFncomms14911_CR13","doi-asserted-by":"publisher","first-page":"5672","DOI":"10.1093\/emboj\/16.18.5672","volume":"16","author":"K Peukert","year":"1997","unstructured":"Peukert, K. et al. An alternative pathway for gene regulation by Myc. EMBO J. 16, 5672\u20135686 (1997).","journal-title":"EMBO J."},{"key":"BFncomms14911_CR14","doi-asserted-by":"publisher","first-page":"1733","DOI":"10.1128\/MCB.20.5.1733-1746.2000","volume":"20","author":"A Kobayashi","year":"2000","unstructured":"Kobayashi, A. et al. A combinatorial code for gene expression generated by transcription factor Bach2 and MAZR (MAZ-related factor) through the BTB\/POZ domain. Mol. Cell. Biol. 20, 1733\u20131746 (2000).","journal-title":"Mol. Cell. Biol."},{"key":"BFncomms14911_CR15","doi-asserted-by":"publisher","first-page":"1161","DOI":"10.1002\/j.1460-2075.1993.tb05757.x","volume":"12","author":"Z Chen","year":"1993","unstructured":"Chen, Z. et al. Fusion between a novel Kruppel-like zinc finger gene and the retinoic acid receptor-alpha locus due to a variant t(11;17) translocation associated with acute promyelocytic leukaemia. EMBO J. 12, 1161\u20131167 (1993).","journal-title":"EMBO J."},{"key":"BFncomms14911_CR16","doi-asserted-by":"publisher","first-page":"74","DOI":"10.3389\/fonc.2012.00074","volume":"2","author":"BA Suliman","year":"2012","unstructured":"Suliman, B. A., Xu, D. & Williams, B. R. The promyelocytic leukemia zinc finger protein: two decades of molecular oncology. Front. Oncol. 2, 74 (2012).","journal-title":"Front. Oncol."},{"key":"BFncomms14911_CR17","doi-asserted-by":"publisher","first-page":"4465","DOI":"10.1073\/pnas.012025199","volume":"99","author":"AI Su","year":"2002","unstructured":"Su, A. I. et al. Large-scale analysis of the human and mouse transcriptomes. Proc. Natl Acad. Sci. USA 99, 4465\u20134470 (2002).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFncomms14911_CR18","doi-asserted-by":"publisher","first-page":"R139","DOI":"10.1186\/gb-2008-9-9-r139","volume":"9","author":"S Kilpinen","year":"2008","unstructured":"Kilpinen, S. et al. Systematic bioinformatic analysis of expression levels of 17,330 human genes across 9,783 samples from 175 types of healthy and pathological tissues. Genome Biol. 9, R139 (2008).","journal-title":"Genome Biol."},{"key":"BFncomms14911_CR19","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1128\/MCB.19.1.680","volume":"19","author":"C Tang","year":"1999","unstructured":"Tang, C., Westling, J. & Seto, E. trans repression of the human metallothionein IIA gene promoter by PZ120, a novel 120-kilodalton zinc finger protein. Mol. Cell. Biol. 19, 680\u2013689 (1999).","journal-title":"Mol. Cell. Biol."},{"key":"BFncomms14911_CR20","first-page":"943","volume":"18","author":"Y Liu","year":"2007","unstructured":"Liu, Y. et al. Identification of differential expression of genes in hepatocellular carcinoma by suppression subtractive hybridization combined cDNA microarray. Oncol. Rep. 18, 943\u2013951 (2007).","journal-title":"Oncol. Rep."},{"key":"BFncomms14911_CR21","doi-asserted-by":"publisher","first-page":"2403","DOI":"10.1101\/gad.12.15.2403","volume":"12","author":"C Nerlov","year":"1998","unstructured":"Nerlov, C. & Graf, T. PU.1 induces myeloid lineage commitment in multipotent hematopoietic progenitors. Genes Dev. 12, 2403\u20132412 (1998).","journal-title":"Genes Dev."},{"key":"BFncomms14911_CR22","doi-asserted-by":"publisher","first-page":"109","DOI":"10.1016\/S1074-7613(02)00501-0","volume":"18","author":"H Hock","year":"2003","unstructured":"Hock, H. et al. Intrinsic requirement for zinc finger transcription factor Gfi-1 in neutrophil differentiation. Immunity 18, 109\u2013120 (2003).","journal-title":"Immunity"},{"key":"BFncomms14911_CR23","doi-asserted-by":"publisher","first-page":"532","DOI":"10.1016\/j.stem.2010.07.016","volume":"7","author":"NK Wilson","year":"2010","unstructured":"Wilson, N. K. et al. Combinatorial transcriptional control in blood stem\/progenitor cells: genome-wide analysis of ten major transcriptional regulators. Cell Stem Cell 7, 532\u2013544 (2010).","journal-title":"Cell Stem Cell"},{"key":"BFncomms14911_CR24","doi-asserted-by":"publisher","first-page":"37","DOI":"10.1038\/nature14374","volume":"520","author":"KT Bieging","year":"2015","unstructured":"Bieging, K. T. & Attardi, L. D. Cancer: a piece of the p53 puzzle. Nature 520, 37\u201338 (2015).","journal-title":"Nature"},{"key":"BFncomms14911_CR25","doi-asserted-by":"publisher","first-page":"393","DOI":"10.1038\/nrm4007","volume":"16","author":"F Kruiswijk","year":"2015","unstructured":"Kruiswijk, F., Labuschagne, C. F. & Vousden, K. H. p53 in survival, death and metabolic health: a lifeguard with a licence to kill. Nat. Rev. Mol. Cell Biol. 16, 393\u2013405 (2015).","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"BFncomms14911_CR26","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1101\/gad.1761609","volume":"23","author":"J Chen","year":"2009","unstructured":"Chen, J. et al. p53 isoform delta113p53 is a p53 target gene that antagonizes p53 apoptotic activity via BclxL activation in zebrafish. Genes Dev. 23, 278\u2013290 (2009).","journal-title":"Genes Dev."},{"key":"BFncomms14911_CR27","doi-asserted-by":"publisher","first-page":"R82","DOI":"10.1186\/gb-2005-6-10-r82","volume":"6","author":"PJ Stogios","year":"2005","unstructured":"Stogios, P. J., Downs, G. S., Jauhal, J. J., Nandra, S. K. & Prive, G. G. Sequence and structural analysis of BTB domain proteins. Genome Biol. 6, R82 (2005).","journal-title":"Genome Biol."},{"key":"BFncomms14911_CR28","doi-asserted-by":"publisher","first-page":"9502","DOI":"10.1128\/JVI.00159-09","volume":"83","author":"SP Moore","year":"2009","unstructured":"Moore, S. P. & Garfinkel, D. J. Functional analysis of N-terminal residues of ty1 integrase. J. Virol. 83, 9502\u20139511 (2009).","journal-title":"J. Virol."},{"key":"BFncomms14911_CR29","doi-asserted-by":"publisher","first-page":"7040","DOI":"10.1074\/jbc.M511416200","volume":"281","author":"G Mendiratta","year":"2006","unstructured":"Mendiratta, G., Eriksson, P. R., Shen, C. H. & Clark, D. J. The DNA-binding domain of the yeast Spt10p activator includes a zinc finger that is homologous to foamy virus integrase. J. Biol. Chem. 281, 7040\u20137048 (2006).","journal-title":"J. Biol. Chem."},{"key":"BFncomms14911_CR30","doi-asserted-by":"publisher","first-page":"1597","DOI":"10.1093\/oxfordjournals.molbev.a003947","volume":"18","author":"C Llorens","year":"2001","unstructured":"Llorens, C. & Marin, I. A mammalian gene evolved from the integrase domain of an LTR retrotransposon. Mol. Biol. Evol. 18, 1597\u20131600 (2001).","journal-title":"Mol. Biol. Evol."},{"key":"BFncomms14911_CR31","doi-asserted-by":"publisher","first-page":"2167","DOI":"10.1093\/molbev\/msp127","volume":"26","author":"A Marco","year":"2009","unstructured":"Marco, A. & Marin, I. CGIN1: a retroviral contribution to mammalian genomes. Mol. Biol. Evol. 26, 2167\u20132170 (2009).","journal-title":"Mol. Biol. Evol."},{"key":"BFncomms14911_CR32","doi-asserted-by":"publisher","first-page":"228","DOI":"10.1038\/nature13585","volume":"514","author":"JL Van Nostrand","year":"2014","unstructured":"Van Nostrand, J. L. et al. Inappropriate p53 activation during development induces features of CHARGE syndrome. Nature 514, 228\u2013232 (2014).","journal-title":"Nature"},{"key":"BFncomms14911_CR33","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/j.devcel.2014.11.018","volume":"32","author":"FO Kok","year":"2015","unstructured":"Kok, F. O. et al. Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish. Dev. Cell 32, 97\u2013108 (2015).","journal-title":"Dev. Cell"},{"key":"BFncomms14911_CR34","doi-asserted-by":"publisher","first-page":"230","DOI":"10.1038\/nature14580","volume":"524","author":"A Rossi","year":"2015","unstructured":"Rossi, A. et al. Genetic compensation induced by deleterious mutations but not gene knockdowns. Nature 524, 230\u2013233 (2015).","journal-title":"Nature"},{"key":"BFncomms14911_CR35","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/S0092-8674(00)81646-3","volume":"99","author":"J Celli","year":"1999","unstructured":"Celli, J. et al. Heterozygous germline mutations in the p53 homolog p63 are the cause of EEC syndrome. Cell 99, 143\u2013153 (1999).","journal-title":"Cell"},{"key":"BFncomms14911_CR36","doi-asserted-by":"publisher","first-page":"714","DOI":"10.1038\/19539","volume":"398","author":"A Yang","year":"1999","unstructured":"Yang, A. et al. p63 is essential for regenerative proliferation in limb, craniofacial and epithelial development. Nature 398, 714\u2013718 (1999).","journal-title":"Nature"},{"key":"BFncomms14911_CR37","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1038\/35003607","volume":"404","author":"A Yang","year":"2000","unstructured":"Yang, A. et al. p73-deficient mice have neurological, pheromonal and inflammatory defects but lack spontaneous tumours. Nature 404, 99\u2013103 (2000).","journal-title":"Nature"},{"key":"BFncomms14911_CR38","doi-asserted-by":"publisher","first-page":"pe38","DOI":"10.1126\/scisignal.2003477","volume":"5","author":"D Ruggero","year":"2012","unstructured":"Ruggero, D. Revisiting the nucleolus: from marker to dynamic integrator of cancer signaling. Sci. Signal 5, pe38 (2012).","journal-title":"Sci. Signal"},{"key":"BFncomms14911_CR39","doi-asserted-by":"publisher","first-page":"89","DOI":"10.1053\/j.seminhematol.2011.02.005","volume":"48","author":"SR Ellis","year":"2011","unstructured":"Ellis, S. R. & Gleizes, P. E. Diamond Blackfan anemia: ribosomal proteins going rogue. Sem. Hematol. 48, 89\u201396 (2011).","journal-title":"Sem. Hematol."},{"key":"BFncomms14911_CR40","doi-asserted-by":"publisher","first-page":"5228","DOI":"10.1182\/blood-2008-01-132290","volume":"112","author":"N Danilova","year":"2008","unstructured":"Danilova, N., Sakamoto, K. M. & Lin, S. Ribosomal protein S19 deficiency in zebrafish leads to developmental abnormalities and defective erythropoiesis through activation of p53 protein family. Blood 112, 5228\u20135237 (2008).","journal-title":"Blood"},{"key":"BFncomms14911_CR41","doi-asserted-by":"publisher","first-page":"3204","DOI":"10.1093\/hmg\/ddn216","volume":"17","author":"T Uechi","year":"2008","unstructured":"Uechi, T. et al. Deficiency of ribosomal protein S19 during early embryogenesis leads to reduction of erythrocytes in a zebrafish model of Diamond-Blackfan anemia. Hum. Mol. Genet. 17, 3204\u20133211 (2008).","journal-title":"Hum. Mol. Genet."},{"key":"BFncomms14911_CR42","doi-asserted-by":"publisher","first-page":"13659","DOI":"10.1073\/pnas.93.24.13659","volume":"93","author":"R Zheng","year":"1996","unstructured":"Zheng, R., Jenkins, T. M. & Craigie, R. Zinc folds the N-terminal domain of HIV-1 integrase, promotes multimerization, and enhances catalytic activity. Proc. Natl Acad. Sci. USA 93, 13659\u201313664 (1996).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFncomms14911_CR43","doi-asserted-by":"crossref","first-page":"9639","DOI":"10.1016\/S0021-9258(19)50138-7","volume":"267","author":"CJ Burke","year":"1992","unstructured":"Burke, C. J. et al. Structural implications of spectroscopic characterization of a putative zinc finger peptide from HIV-1 integrase. J. Biol. Chem. 267, 9639\u20139644 (1992).","journal-title":"J. Biol. Chem."},{"key":"BFncomms14911_CR44","doi-asserted-by":"publisher","first-page":"2669","DOI":"10.1002\/pro.5560071221","volume":"7","author":"M Cai","year":"1998","unstructured":"Cai, M. et al. Solution structure of the His12 --&gt; Cys mutant of the N-terminal zinc binding domain of HIV-1 integrase complexed to cadmium. Protein Sci. 7, 2669\u20132674 (1998).","journal-title":"Protein Sci."},{"key":"BFncomms14911_CR45","doi-asserted-by":"publisher","first-page":"567","DOI":"10.1038\/nsb0797-567","volume":"4","author":"M Cai","year":"1997","unstructured":"Cai, M. et al. Solution structure of the N-terminal zinc binding domain of HIV-1 integrase. Nat. Struct. Biol. 4, 567\u2013577 (1997).","journal-title":"Nat. Struct. Biol."},{"key":"BFncomms14911_CR46","doi-asserted-by":"publisher","first-page":"79","DOI":"10.1002\/iub.2","volume":"60","author":"A di Masi","year":"2008","unstructured":"di Masi, A. May a missense mutation be more deleterious than a truncating mutation? IUBMB Life 60, 79\u201381 (2008).","journal-title":"IUBMB Life"},{"key":"BFncomms14911_CR47","doi-asserted-by":"publisher","first-page":"12815","DOI":"10.1021\/ja073902+","volume":"129","author":"AR Reddi","year":"2007","unstructured":"Reddi, A. R., Guzman, T. R., Breece, R. M., Tierney, D. L. & Gibney, B. R. Deducing the energetic cost of protein folding in zinc finger proteins using designed metallopeptides. J. Am. Chem. Soc. 129, 12815\u201312827 (2007).","journal-title":"J. Am. Chem. Soc."},{"key":"BFncomms14911_CR48","doi-asserted-by":"publisher","first-page":"892","DOI":"10.1021\/ja075302g","volume":"130","author":"W Li","year":"2008","unstructured":"Li, W., Zhang, J., Wang, J. & Wang, W. Metal-coupled folding of Cys2His2 zinc-finger. J. Am. Chem. Soc. 130, 892\u2013900 (2008).","journal-title":"J. Am. Chem. Soc."},{"key":"BFncomms14911_CR49","doi-asserted-by":"publisher","first-page":"506","DOI":"10.1016\/j.cub.2006.01.047","volume":"16","author":"BM Hogan","year":"2006","unstructured":"Hogan, B. M. et al. Specification of the primitive myeloid precursor pool requires signaling through Alk8 in zebrafish. Curr. Biol. 16, 506\u2013511 (2006).","journal-title":"Curr. Biol."},{"key":"BFncomms14911_CR50","doi-asserted-by":"publisher","first-page":"549","DOI":"10.1080\/01902140701756778","volume":"33","author":"SA Renshaw","year":"2007","unstructured":"Renshaw, S. A., Loynes, C. A., Elworthy, S., Ingham, P. W. & Whyte, M. K. B. Modeling inflammation in the zebrafish: how a fish can help us understand lung disease. Exp. Lung Res. 33, 549\u2013554 (2007).","journal-title":"Exp. Lung Res."},{"key":"BFncomms14911_CR51","doi-asserted-by":"publisher","first-page":"42","DOI":"10.1186\/1471-213X-7-42","volume":"7","author":"C Hall","year":"2007","unstructured":"Hall, C., Flores, M. V., Storm, T., Crosier, K. & Crosier, P. The zebrafish lysozyme C promoter drives myeloid-specific expression in transgenic fish. BMC Dev. Biol. 7, 42 (2007).","journal-title":"BMC Dev. Biol."},{"key":"BFncomms14911_CR52","doi-asserted-by":"publisher","first-page":"270","DOI":"10.1016\/j.cbpa.2009.06.024","volume":"154","author":"N Yaqoob","year":"2009","unstructured":"Yaqoob, N., Holotta, M., Prem, C., Kopp, R. & Schwerte, T. Ontogenetic development of erythropoiesis can be studied non-invasively in GATA-1:DsRed transgenic zebrafish. Comp. Biochem. Physiol. A Mol. Integr. Physiol. 154, 270\u2013278 (2009).","journal-title":"Comp. Biochem. Physiol. A Mol. Integr. Physiol."},{"key":"BFncomms14911_CR53","doi-asserted-by":"publisher","first-page":"321","DOI":"10.3389\/fncel.2015.00321","volume":"9","author":"M Morsch","year":"2015","unstructured":"Morsch, M. et al. In vivo characterization of microglial engulfment of dying neurons in the zebrafish spinal cord. Front Cell. Neurosci. 9, 321 (2015).","journal-title":"Front Cell. Neurosci."},{"key":"BFncomms14911_CR54","doi-asserted-by":"publisher","first-page":"e49","DOI":"10.1182\/blood-2010-10-314120","volume":"117","author":"F Ellett","year":"2011","unstructured":"Ellett, F., Pase, L., Hayman, J. W., Andrianopoulos, A. & Lieschke, G. J. mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish. Blood 117, e49\u2013e56 (2011).","journal-title":"Blood"},{"key":"BFncomms14911_CR55","doi-asserted-by":"publisher","first-page":"2150","DOI":"10.1016\/j.febslet.2013.05.030","volume":"587","author":"MC Keightley","year":"2013","unstructured":"Keightley, M. C. et al. In vivo mutation of pre-mRNA processing factor 8 (Prpf8) affects transcript splicing, cell survival and myeloid differentiation. FEBS Lett. 587, 2150\u20132157 (2013).","journal-title":"FEBS Lett."},{"key":"BFncomms14911_CR56","doi-asserted-by":"publisher","first-page":"e98186","DOI":"10.1371\/journal.pone.0098186","volume":"9","author":"JA Gagnon","year":"2014","unstructured":"Gagnon, J. A. et al. Efficient mutagenesis by Cas9 protein-mediated oligonucleotide insertion and large-scale assessment of single-guide RNAs. PLoS ONE 9, e98186 (2014).","journal-title":"PLoS ONE"},{"key":"BFncomms14911_CR57","doi-asserted-by":"publisher","first-page":"249","DOI":"10.1093\/biostatistics\/4.2.249","volume":"4","author":"RA Irizarry","year":"2003","unstructured":"Irizarry, R. A. et al. Exploration, normalization, and summaries of high density oligonucleotide array probe level data. Biostatistics 4, 249\u2013264 (2003).","journal-title":"Biostatistics"},{"key":"BFncomms14911_CR58","doi-asserted-by":"publisher","first-page":"274","DOI":"10.1006\/dbio.2002.0657","volume":"246","author":"GJ Lieschke","year":"2002","unstructured":"Lieschke, G. J. et al. Zebrafish SPI-1 (PU.1) marks a site of myeloid development independent of primitive erythropoiesis: implications for axial patterning. Dev. Biol. 246, 274\u2013295 (2002).","journal-title":"Dev. Biol."},{"key":"BFncomms14911_CR59","doi-asserted-by":"publisher","first-page":"676","DOI":"10.1038\/nmeth.2019","volume":"9","author":"J Schindelin","year":"2012","unstructured":"Schindelin, J. et al. Fiji: an open-source platform for biological-image analysis. Nat. Methods 9, 676\u2013682 (2012).","journal-title":"Nat. Methods"},{"key":"BFncomms14911_CR60","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1016\/B978-0-12-391856-7.00046-9","volume":"506","author":"F Ellett","year":"2012","unstructured":"Ellett, F. & Lieschke, G. J. Computational quantification of fluorescent leukocyte numbers in zebrafish embryos. Methods Enzymol. 506, 425\u2013435 (2012).","journal-title":"Methods Enzymol."},{"key":"BFncomms14911_CR61","doi-asserted-by":"publisher","first-page":"e108","DOI":"10.1093\/nar\/gkt214","volume":"41","author":"Y Liao","year":"2013","unstructured":"Liao, Y., Smyth, G. K. & Shi, W. The Subread aligner: fast, accurate and scalable read mapping by seed-and-vote. Nucleic Acids Res. 41, e108 (2013).","journal-title":"Nucleic Acids Res."},{"key":"BFncomms14911_CR62","doi-asserted-by":"publisher","first-page":"2947","DOI":"10.1093\/bioinformatics\/btm404","volume":"23","author":"MA Larkin","year":"2007","unstructured":"Larkin, M. A. et al. Clustal W and Clustal X version 2.0. Bioinformatics 23, 2947\u20132948 (2007).","journal-title":"Bioinformatics"},{"key":"BFncomms14911_CR63","doi-asserted-by":"crossref","unstructured":"Eswar, N. et al. Comparative protein structure modeling using MODELLER. Curr. Protoc. Protein Sci Chapter 2, Unit 2.9 (2007).","DOI":"10.1002\/0471140864.ps0209s50"},{"key":"BFncomms14911_CR64","doi-asserted-by":"publisher","first-page":"4029","DOI":"10.1093\/emboj\/17.14.4029","volume":"17","author":"M Gering","year":"1998","unstructured":"Gering, M., Rodaway, A. R., Gottgens, B., Patient, R. K. & Green, A. R. The SCL gene specifies haemangioblast development from early mesoderm. EMBO J. 17, 4029\u20134045 (1998).","journal-title":"EMBO J."},{"key":"BFncomms14911_CR65","doi-asserted-by":"publisher","first-page":"220","DOI":"10.1016\/j.mod.2008.11.001","volume":"126","author":"F Ellett","year":"2009","unstructured":"Ellett, F., Kile, B. T. & Lieschke, G. J. The role of the ETS factor erg in zebrafish vasculogenesis. Mech. Dev. 126, 220\u2013229 (2009).","journal-title":"Mech. Dev."},{"key":"BFncomms14911_CR66","doi-asserted-by":"publisher","first-page":"2611","DOI":"10.1182\/blood.V97.9.2611","volume":"97","author":"SE Lyons","year":"2001","unstructured":"Lyons, S. E., Shue, B. C., Oates, A. C., Zon, L. I. & Liu, P. P. A novel myeloid-restricted zebrafish CCAAT\/enhancer-binding protein with a potent transcriptional activation domain. Blood 97, 2611\u20132617 (2001).","journal-title":"Blood"},{"key":"BFncomms14911_CR67","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1016\/j.ydbio.2012.08.015","volume":"373","author":"JD Cooney","year":"2013","unstructured":"Cooney, J. D. et al. Teleost growth factor independence (gfi) genes differentially regulate successive waves of hematopoiesis. Dev. Biol. 373, 431\u2013441 (2013).","journal-title":"Dev. Biol."},{"key":"BFncomms14911_CR68","doi-asserted-by":"publisher","first-page":"801","DOI":"10.1016\/j.cell.2006.03.032","volume":"125","author":"J Lim","year":"2006","unstructured":"Lim, J. et al. A protein-protein interaction network for human inherited ataxias and disorders of Purkinje cell degeneration. Cell 125, 801\u2013814 (2006).","journal-title":"Cell"},{"key":"BFncomms14911_CR69","doi-asserted-by":"publisher","first-page":"6982","DOI":"10.1158\/0008-5472.CAN-06-0511","volume":"66","author":"S Wang","year":"2006","unstructured":"Wang, S. & El-Deiry, W. S. p73 or p53 directly regulates human p53 transcription to maintain cell cycle checkpoints. Cancer Res. 66, 6982\u20136989 (2006).","journal-title":"Cancer Res."},{"key":"BFncomms14911_CR70","doi-asserted-by":"publisher","first-page":"253","DOI":"10.1002\/aja.1002030302","volume":"203","author":"CB Kimmel","year":"1995","unstructured":"Kimmel, C. B., Ballard, W. W., Kimmel, S. R., Ullmann, B. & Schilling, T. F. Stages of embryonic development of the zebrafish. Dev. Dyn. 203, 253\u2013310 (1995).","journal-title":"Dev. Dyn."}],"container-title":["Nature Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/ncomms14911.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/ncomms14911","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/doifinder\/10.1038\/ncomms14911","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/www.nature.com\/articles\/ncomms14911.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,6,17]],"date-time":"2025-06-17T21:09:34Z","timestamp":1750194574000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/ncomms14911"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,4,6]]},"references-count":70,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,4,28]]}},"alternative-id":["BFncomms14911"],"URL":"https:\/\/doi.org\/10.1038\/ncomms14911","relation":{},"ISSN":["2041-1723"],"issn-type":[{"value":"2041-1723","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,4,6]]},"assertion":[{"value":"4 July 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"13 February 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"6 April 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"14911"}}