{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T04:28:15Z","timestamp":1791088095276,"version":"4.1.0"},"reference-count":30,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,5,4]],"date-time":"2017-05-04T00:00:00Z","timestamp":1493856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,5,4]],"date-time":"2017-05-04T00:00:00Z","timestamp":1493856000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Commun"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Fungi are a valuable source of enzymatic diversity and therapeutic natural products including antibiotics. Here we engineer the baker\u2019s yeast\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    to produce and secrete the antibiotic penicillin, a beta-lactam nonribosomal peptide, by taking genes from a filamentous fungus and directing their efficient expression and subcellular localization. Using synthetic biology tools combined with long-read DNA sequencing, we optimize productivity by 50-fold to produce bioactive yields that allow spent\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    growth media to have antibacterial action against\n                    <jats:italic>Streptococcus<\/jats:italic>\n                    bacteria. This work demonstrates that\n                    <jats:italic>S. cerevisiae<\/jats:italic>\n                    can be engineered to perform the complex biosynthesis of multicellular fungi, opening up the possibility of using yeast to accelerate rational engineering of nonribosomal peptide antibiotics.\n                  <\/jats:p>","DOI":"10.1038\/ncomms15202","type":"journal-article","created":{"date-parts":[[2017,5,4]],"date-time":"2017-05-04T04:59:07Z","timestamp":1493873947000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":99,"title":["Biosynthesis of the antibiotic nonribosomal peptide penicillin in baker\u2019s yeast"],"prefix":"10.1038","volume":"8","author":[{"given":"Ali R.","family":"Awan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin A.","family":"Blount","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David J.","family":"Bell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William M.","family":"Shaw","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jack C.H.","family":"Ho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert M.","family":"McKiernan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5392-976X","authenticated-orcid":false,"given":"Tom","family":"Ellis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,5,4]]},"reference":[{"key":"BFncomms15202_CR1","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/s13225-011-0116-y","volume":"50","author":"AH Aly","year":"2011","unstructured":"Aly, A. H., Debbab, A. & Proksch, P. Fifty years of drug discovery from fungi. Fungal Divers 50, 3\u201319 (2011).","journal-title":"Fungal Divers"},{"key":"BFncomms15202_CR2","doi-asserted-by":"publisher","first-page":"21","DOI":"10.1038\/nrmicro2916","volume":"11","author":"AA Brakhage","year":"2013","unstructured":"Brakhage, A. A. Regulation of fungal secondary metabolism. Nat. Rev. Microbiol. 11, 21\u201332 (2013).","journal-title":"Nat. Rev. Microbiol."},{"key":"BFncomms15202_CR3","doi-asserted-by":"publisher","first-page":"e1003950","DOI":"10.1371\/journal.ppat.1003950","volume":"10","author":"U Kuck","year":"2014","unstructured":"Kuck, U., Bloemendal, S. & Teichert, I. Putting fungi to work: harvesting a cornucopia of drugs, toxins, and antibiotics. 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