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The leukocyte integrin Mac-1 (also known as integrin \u03b1\n                    <jats:sub>M<\/jats:sub>\n                    \u03b2\n                    <jats:sub>2<\/jats:sub>\n                    , or CD11b\/CD18) is crucial for leukocyte recruitment to the endothelium, and Mac-1 engagement of platelet GPIb\u03b1 is required for injury responses in diverse disease models. However, the role of Mac-1 in thrombosis is undefined. Here we report that mice with Mac-1 deficiency (\n                    <jats:italic>Mac-1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    ) or mutation of the Mac-1-binding site for GPIb\u03b1 have delayed thrombosis after carotid artery and cremaster microvascular injury without affecting parameters of haemostasis. Adoptive wild-type leukocyte transfer rescues the thrombosis defect in\n                    <jats:italic>Mac-1<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    mice, and Mac-1-dependent regulation of the transcription factor Foxp1 contributes to thrombosis as evidenced by delayed thrombosis in mice with monocyte-\/macrophage-specific overexpression of Foxp1. Antibody and small-molecule targeting of Mac-1:GPIb\u03b1 inhibits thrombosis. Our data identify a new pathway of thrombosis involving leukocyte Mac-1 and platelet GPIb\u03b1, and suggest that targeting this interaction has anti-thrombotic therapeutic potential with reduced bleeding risk.\n                  <\/jats:p>","DOI":"10.1038\/ncomms15559","type":"journal-article","created":{"date-parts":[[2017,5,30]],"date-time":"2017-05-30T06:27:50Z","timestamp":1496125670000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":156,"title":["Leukocyte integrin Mac-1 regulates thrombosis via interaction with platelet GPIb\u03b1"],"prefix":"10.1038","volume":"8","author":[{"given":"Yunmei","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiyun","family":"Gao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Can","family":"Shi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul W.","family":"Erhardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Pavlovsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry","family":"A. Soloviev","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamila","family":"Bledzka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valentin","family":"Ustinov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liang","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Qin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam D.","family":"Munday","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose","family":"Lopez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward","family":"Plow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel I.","family":"Simon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,5,30]]},"reference":[{"key":"BFncomms15559_CR1","first-page":"e21","volume":"119","author":"D Lloyd-Jones","year":"2009","unstructured":"Lloyd-Jones, D. et al. 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