{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:31:04Z","timestamp":1790735464616,"version":"4.1.0"},"reference-count":71,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2017,6,9]],"date-time":"2017-06-09T00:00:00Z","timestamp":1496966400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2017,6,9]],"date-time":"2017-06-09T00:00:00Z","timestamp":1496966400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Commun"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Mitochondrial division is critical for the maintenance and regulation of mitochondrial function, quality and distribution. This process is controlled by cytosolic actin-based constriction machinery and dynamin-related protein 1 (Drp1) on mitochondrial outer membrane (OMM). Although mitochondrial physiology, including oxidative phosphorylation, is also important for efficient mitochondrial division, morphological alterations of the mitochondrial inner-membrane (IMM) have not been clearly elucidated. Here we report spontaneous and repetitive constriction of mitochondrial inner compartment (CoMIC) associated with subsequent division in neurons. Although CoMIC is potentiated by inhibition of Drp1 and occurs at the potential division spots contacting the endoplasmic reticulum, it appears on IMM independently of OMM. Intra-mitochondrial influx of Ca\n                    <jats:sup>2+<\/jats:sup>\n                    induces and potentiates CoMIC, and leads to K\n                    <jats:sup>+<\/jats:sup>\n                    -mediated mitochondrial bulging and depolarization. Synergistically, optic atrophy 1 (Opa1) also regulates CoMIC via controlling Mic60-mediated OMM\u2013IMM tethering. Therefore, we propose that CoMIC is a priming event for efficient mitochondrial division.\n                  <\/jats:p>","DOI":"10.1038\/ncomms15754","type":"journal-article","created":{"date-parts":[[2017,6,9]],"date-time":"2017-06-09T11:26:46Z","timestamp":1497007606000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":178,"title":["Constriction of the mitochondrial inner compartment is a priming event for mitochondrial division"],"prefix":"10.1038","volume":"8","author":[{"given":"Bongki","family":"Cho","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyo Min","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Youhwa","family":"Jo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hee Dae","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myungjae","family":"Song","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheil","family":"Moon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4693-738X","authenticated-orcid":false,"given":"Hyongbum","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kyungjin","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromi","family":"Sesaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Im Joo","family":"Rhyu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hyun","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1792-4894","authenticated-orcid":false,"given":"Woong","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,6,9]]},"reference":[{"key":"BFncomms15754_CR1","doi-asserted-by":"publisher","first-page":"1062","DOI":"10.1126\/science.1219855","volume":"337","author":"RJ Youle","year":"2012","unstructured":"Youle, R. J. & van der Bliek, A. M. Mitochondrial fission, fusion, and stress. Science 337, 1062\u20131065 (2012).","journal-title":"Science"},{"key":"BFncomms15754_CR2","doi-asserted-by":"publisher","first-page":"97","DOI":"10.1016\/S1937-6448(08)01203-3","volume":"271","author":"T Kuroiwa","year":"2008","unstructured":"Kuroiwa, T. et al. Vesicle, mitochondrial, and plastid division machineries with emphasis on dynamin and electron-dense rings. Int. Rev. Cell Mol. Biol. 271, 97\u2013152 (2008).","journal-title":"Int. Rev. Cell Mol. Biol."},{"key":"BFncomms15754_CR3","doi-asserted-by":"publisher","first-page":"265","DOI":"10.1146\/annurev-genet-110410-132529","volume":"46","author":"DC Chan","year":"2012","unstructured":"Chan, D. C. Fusion and fission: interlinked processes critical for mitochondrial health. Annu. Rev. Genet. 46, 265\u2013287 (2012).","journal-title":"Annu. Rev. Genet."},{"key":"BFncomms15754_CR4","doi-asserted-by":"publisher","first-page":"659","DOI":"10.1091\/mbc.e12-10-0721","volume":"24","author":"OC Loson","year":"2013","unstructured":"Loson, O. C., Song, Z., Chen, H. & Chan, D. C. Fis1, Mff, MiD49, and MiD51 mediate Drp1 recruitment in mitochondrial fission. Mol. Biol. Cell 24, 659\u2013667 (2013).","journal-title":"Mol. Biol. Cell"},{"key":"BFncomms15754_CR5","doi-asserted-by":"publisher","first-page":"1021","DOI":"10.1083\/jcb.200506078","volume":"170","author":"E Ingerman","year":"2005","unstructured":"Ingerman, E. et al. Dnm1 forms spirals that are structurally tailored to fit mitochondria. J. Cell Biol. 170, 1021\u20131027 (2005).","journal-title":"J. Cell Biol."},{"key":"BFncomms15754_CR6","doi-asserted-by":"publisher","first-page":"20","DOI":"10.1038\/nsmb.1949","volume":"18","author":"JA Mears","year":"2011","unstructured":"Mears, J. A. et al. Conformational changes in Dnm1 support a contractile mechanism for mitochondrial fission. Nat. Struct. Mol. Biol. 18, 20\u201326 (2011).","journal-title":"Nat. Struct. Mol. Biol."},{"key":"BFncomms15754_CR7","doi-asserted-by":"publisher","first-page":"358","DOI":"10.1126\/science.1207385","volume":"334","author":"JR Friedman","year":"2011","unstructured":"Friedman, J. R. et al. ER tubules mark sites of mitochondrial division. Science 334, 358\u2013362 (2011).","journal-title":"Science"},{"key":"BFncomms15754_CR8","doi-asserted-by":"publisher","first-page":"464","DOI":"10.1126\/science.1228360","volume":"339","author":"F Korobova","year":"2013","unstructured":"Korobova, F., Ramabhadran, V. & Higgs, H. N. An actin-dependent step in mitochondrial fission mediated by the ER-associated formin INF2. Science 339, 464\u2013467 (2013).","journal-title":"Science"},{"key":"BFncomms15754_CR9","doi-asserted-by":"publisher","first-page":"139","DOI":"10.1038\/nature20555","volume":"540","author":"JE Lee","year":"2016","unstructured":"Lee, J. E., Westrate, L. M., Wu, H., Page, C. & Voeltz, G. K. Multiple dynamin family members collaborate to drive mitochondrial division. Nature 540, 139\u2013143 (2016).","journal-title":"Nature"},{"key":"BFncomms15754_CR10","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1038\/nrm3266","volume":"13","author":"SM Ferguson","year":"2012","unstructured":"Ferguson, S. M. & De Camilli, P. Dynamin, a membrane-remodelling GTPase. Nat. Rev. Mol. Cell Biol. 13, 75\u201388 (2012).","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"BFncomms15754_CR11","doi-asserted-by":"publisher","first-page":"773","DOI":"10.1038\/ncb2791","volume":"15","author":"H Li","year":"2013","unstructured":"Li, H. et al. A Bcl-xL-Drp1 complex regulates synaptic vesicle membrane dynamics during endocytosis. Nat. Cell Biol. 15, 773\u2013785 (2013).","journal-title":"Nat. Cell Biol."},{"key":"BFncomms15754_CR12","doi-asserted-by":"publisher","first-page":"e52","DOI":"10.1371\/journal.pgen.0010052","volume":"1","author":"NC Elde","year":"2005","unstructured":"Elde, N. C., Morgan, G., Winey, M., Sperling, L. & Turkewitz, A. P. Elucidation of clathrin-mediated endocytosis in tetrahymena reveals an evolutionarily convergent recruitment of dynamin. PLoS Genet. 1, e52 (2005).","journal-title":"PLoS Genet."},{"key":"BFncomms15754_CR13","doi-asserted-by":"publisher","first-page":"2146","DOI":"10.1073\/pnas.0436886100","volume":"100","author":"K Nishida","year":"2003","unstructured":"Nishida, K. et al. Dynamic recruitment of dynamin for final mitochondrial severance in a primitive red alga. Proc. Natl Acad. Sci. USA 100, 2146\u20132151 (2003).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFncomms15754_CR14","doi-asserted-by":"publisher","first-page":"1953","DOI":"10.1091\/mbc.e02-10-0657","volume":"14","author":"A Legesse-Miller","year":"2003","unstructured":"Legesse-Miller, A., Massol, R. H. & Kirchhausen, T. Constriction and Dnm1p recruitment are distinct processes in mitochondrial fission. Mol. Biol. Cell 14, 1953\u20131963 (2003).","journal-title":"Mol. Biol. Cell"},{"key":"BFncomms15754_CR15","doi-asserted-by":"publisher","first-page":"815","DOI":"10.1016\/S1097-2765(00)80391-3","volume":"4","author":"AM Labrousse","year":"1999","unstructured":"Labrousse, A. M., Zappaterra, M. D., Rube, D. A. & van der Bliek, A. M. C. elegans dynamin-related protein DRP-1 controls severing of the mitochondrial outer membrane. Mol. Cell 4, 815\u2013826 (1999).","journal-title":"Mol. Cell"},{"key":"BFncomms15754_CR16","doi-asserted-by":"publisher","first-page":"958","DOI":"10.1038\/ncb1907","volume":"11","author":"N Ishihara","year":"2009","unstructured":"Ishihara, N. et al. Mitochondrial fission factor Drp1 is essential for embryonic development and synapse formation in mice. Nat. Cell Biol. 11, 958\u2013966 (2009).","journal-title":"Nat. Cell Biol."},{"key":"BFncomms15754_CR17","doi-asserted-by":"publisher","first-page":"805","DOI":"10.1083\/jcb.200903065","volume":"186","author":"J Wakabayashi","year":"2009","unstructured":"Wakabayashi, J. et al. The dynamin-related GTPase Drp1 is required for embryonic and brain development in mice. J. Cell Biol. 186, 805\u2013816 (2009).","journal-title":"J. Cell Biol."},{"key":"BFncomms15754_CR18","doi-asserted-by":"publisher","first-page":"1455","DOI":"10.1002\/ar.22523","volume":"295","author":"H Fujioka","year":"2012","unstructured":"Fujioka, H., Tandler, B. & Hoppel, C. L. Mitochondrial division in rat cardiomyocytes: an electron microscope study. Anat. Rec. 295, 1455\u20131461 (2012).","journal-title":"Anat. Rec."},{"key":"BFncomms15754_CR19","doi-asserted-by":"publisher","first-page":"C157","DOI":"10.1152\/ajpcell.00272.2006","volume":"292","author":"A Kaasik","year":"2007","unstructured":"Kaasik, A., Safiulina, D., Zharkovsky, A. & Veksler, V. Regulation of mitochondrial matrix volume. Am. J. Physiol. Cell Physiol. 292, C157\u2013C163 (2007).","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"BFncomms15754_CR20","doi-asserted-by":"publisher","first-page":"505","DOI":"10.1038\/nrn2417","volume":"9","author":"AB Knott","year":"2008","unstructured":"Knott, A. B., Perkins, G., Schwarzenbacher, R. & Bossy-Wetzel, E. Mitochondrial fragmentation in neurodegeneration. Nat. Rev. Neurosci. 9, 505\u2013518 (2008).","journal-title":"Nat. Rev. Neurosci."},{"key":"BFncomms15754_CR21","doi-asserted-by":"publisher","first-page":"1589","DOI":"10.1038\/emboj.2009.89","volume":"28","author":"D Tondera","year":"2009","unstructured":"Tondera, D. et al. SLP-2 is required for stress-induced mitochondrial hyperfusion. EMBO J. 28, 1589\u20131600 (2009).","journal-title":"EMBO J."},{"key":"BFncomms15754_CR22","doi-asserted-by":"publisher","first-page":"2313","DOI":"10.1242\/jcs.144337","volume":"127","author":"TD MacVicar","year":"2014","unstructured":"MacVicar, T. D. & Lane, J. D. Impaired OMA1-dependent cleavage of OPA1 and reduced DRP1 fission activity combine to prevent mitophagy in cells that are dependent on oxidative phosphorylation. J. Cell Sci. 127, 2313\u20132325 (2014).","journal-title":"J. Cell Sci."},{"key":"BFncomms15754_CR23","doi-asserted-by":"publisher","first-page":"555","DOI":"10.1038\/nm.3520","volume":"20","author":"MO Breckwoldt","year":"2014","unstructured":"Breckwoldt, M. O. et al. Multiparametric optical analysis of mitochondrial redox signals during neuronal physiology and pathology in vivo. Nat. Med. 20, 555\u2013560 (2014).","journal-title":"Nat. Med."},{"key":"BFncomms15754_CR24","doi-asserted-by":"publisher","first-page":"11862","DOI":"10.1074\/jbc.M113.533299","volume":"289","author":"H Lee","year":"2014","unstructured":"Lee, H. & Yoon, Y. Transient contraction of mitochondria induces depolarization through the inner membrane dynamin OPA1 protein. J. Biol. Chem. 289, 11862\u201311872 (2014).","journal-title":"J. Biol. Chem."},{"key":"BFncomms15754_CR25","doi-asserted-by":"publisher","first-page":"18725","DOI":"10.1038\/srep18725","volume":"6","author":"L Zhang","year":"2016","unstructured":"Zhang, L. et al. Altered brain energetics induces mitochondrial fission arrest in Alzheimer's disease. Sci. Rep. 6, 18725 (2016).","journal-title":"Sci. Rep."},{"key":"BFncomms15754_CR26","doi-asserted-by":"publisher","first-page":"635","DOI":"10.1016\/S0960-9822(95)00128-X","volume":"5","author":"R Rizzuto","year":"1995","unstructured":"Rizzuto, R., Brini, M., Pizzo, P., Murgia, M. & Pozzan, T. Chimeric green fluorescent protein as a tool for visualizing subcellular organelles in living cells. Curr. Biol. 5, 635\u2013642 (1995).","journal-title":"Curr. Biol."},{"key":"BFncomms15754_CR27","doi-asserted-by":"publisher","first-page":"e105","DOI":"10.1038\/emm.2014.36","volume":"46","author":"B Cho","year":"2014","unstructured":"Cho, B. et al. CDK5-dependent inhibitory phosphorylation of Drp1 during neuronal maturation. Exp. Mol. Med. 46, e105 (2014).","journal-title":"Exp. Mol. Med."},{"key":"BFncomms15754_CR28","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1016\/S1534-5807(01)00055-7","volume":"1","author":"S Frank","year":"2001","unstructured":"Frank, S. et al. The role of dynamin-related protein 1, a mediator of mitochondrial fission, in apoptosis. Dev. Cell 1, 515\u2013525 (2001).","journal-title":"Dev. Cell"},{"key":"BFncomms15754_CR29","doi-asserted-by":"publisher","first-page":"2894","DOI":"10.1091\/mbc.12.9.2894","volume":"12","author":"Y Yoon","year":"2001","unstructured":"Yoon, Y., Pitts, K. R. & McNiven, M. A. Mammalian dynamin-like protein DLP1 tubulates membranes. Mol. Biol. Cell 12, 2894\u20132905 (2001).","journal-title":"Mol. Biol. Cell"},{"key":"BFncomms15754_CR30","doi-asserted-by":"publisher","first-page":"aaf5549","DOI":"10.1126\/science.aaf5549","volume":"353","author":"SC Lewis","year":"2016","unstructured":"Lewis, S. C., Uchiyama, L. F. & Nunnari, J. ER-mitochondria contacts couple mtDNA synthesis with mitochondrial division in human cells. Science 353, aaf5549 (2016).","journal-title":"Science"},{"key":"BFncomms15754_CR31","doi-asserted-by":"publisher","first-page":"1583","DOI":"10.1091\/mbc.e02-07-0399","volume":"14","author":"N Garrido","year":"2003","unstructured":"Garrido, N. et al. Composition and dynamics of human mitochondrial nucleoids. Mol. Biol. Cell 14, 1583\u20131596 (2003).","journal-title":"Mol. Biol. Cell"},{"key":"BFncomms15754_CR32","doi-asserted-by":"publisher","first-page":"952","DOI":"10.1093\/nar\/gkt988","volume":"42","author":"N Rajala","year":"2014","unstructured":"Rajala, N., Gerhold, J. M., Martinsson, P., Klymov, A. & Spelbrink, J. N. Replication factors transiently associate with mtDNA at the mitochondrial inner membrane to facilitate replication. Nucleic Acids Res. 42, 952\u2013967 (2014).","journal-title":"Nucleic Acids Res."},{"key":"BFncomms15754_CR33","doi-asserted-by":"publisher","first-page":"E2174","DOI":"10.1073\/pnas.1504880112","volume":"112","author":"R Filadi","year":"2015","unstructured":"Filadi, R. et al. Mitofusin 2 ablation increases endoplasmic reticulum-mitochondria coupling. Proc. Natl Acad. Sci. USA 112, E2174\u2013E2181 (2015).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFncomms15754_CR34","doi-asserted-by":"publisher","first-page":"605","DOI":"10.1038\/nature07534","volume":"456","author":"OM de Brito","year":"2008","unstructured":"de Brito, O. M. & Scorrano, L. Mitofusin 2 tethers endoplasmic reticulum to mitochondria. Nature 456, 605\u2013610 (2008).","journal-title":"Nature"},{"key":"BFncomms15754_CR35","doi-asserted-by":"publisher","first-page":"15456","DOI":"10.1073\/pnas.1106862108","volume":"108","author":"KT Chang","year":"2011","unstructured":"Chang, K. T., Niescier, R. F. & Min, K. T. Mitochondrial matrix Ca2+ as an intrinsic signal regulating mitochondrial motility in axons. Proc. Natl Acad. Sci. USA 108, 15456\u201315461 (2011).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFncomms15754_CR36","doi-asserted-by":"publisher","first-page":"C817","DOI":"10.1152\/ajpcell.00139.2004","volume":"287","author":"PS Brookes","year":"2004","unstructured":"Brookes, P. S., Yoon, Y., Robotham, J. L., Anders, M. W. & Sheu, S. S. Calcium, ATP, and ROS: a mitochondrial love-hate triangle. Am. J. Physiol. Cell Physiol. 287, C817\u2013C833 (2004).","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"BFncomms15754_CR37","doi-asserted-by":"publisher","first-page":"4153","DOI":"10.1038\/ncomms5153","volume":"5","author":"J Suzuki","year":"2014","unstructured":"Suzuki, J. et al. Imaging intraorganellar Ca2+ at subcellular resolution using CEPIA. Nat. Commun. 5, 4153 (2014).","journal-title":"Nat. Commun."},{"key":"BFncomms15754_CR38","doi-asserted-by":"publisher","first-page":"e540","DOI":"10.1038\/cddis.2013.7","volume":"4","author":"S Xu","year":"2013","unstructured":"Xu, S. et al. Cadmium induced Drp1-dependent mitochondrial fragmentation by disturbing calcium homeostasis in its hepatotoxicity. Cell Death Dis. 4, e540 (2013).","journal-title":"Cell Death Dis."},{"key":"BFncomms15754_CR39","doi-asserted-by":"publisher","first-page":"1115","DOI":"10.1083\/jcb.200212059","volume":"160","author":"DG Breckenridge","year":"2003","unstructured":"Breckenridge, D. G., Stojanovic, M., Marcellus, R. C. & Shore, G. C. Caspase cleavage product of BAP31 induces mitochondrial fission through endoplasmic reticulum calcium signals, enhancing cytochrome c release to the cytosol. J. Cell Biol. 160, 1115\u20131127 (2003).","journal-title":"J. Cell Biol."},{"key":"BFncomms15754_CR40","doi-asserted-by":"publisher","first-page":"913","DOI":"10.1016\/j.bbabio.2010.03.018","volume":"1797","author":"J Hom","year":"2010","unstructured":"Hom, J., Yu, T., Yoon, Y., Porter, G. & Sheu, S. S. Regulation of mitochondrial fission by intracellular Ca2+ in rat ventricular myocytes. Biochim. Biophys. Acta 1797, 913\u2013921 (2010).","journal-title":"Biochim. Biophys. Acta"},{"key":"BFncomms15754_CR41","doi-asserted-by":"publisher","first-page":"1546","DOI":"10.1038\/sj.emboj.7600592","volume":"24","author":"M Germain","year":"2005","unstructured":"Germain, M., Mathai, J. P., McBride, H. M. & Shore, G. C. Endoplasmic reticulum BIK initiates DRP1-regulated remodelling of mitochondrial cristae during apoptosis. EMBO J. 24, 1546\u20131556 (2005).","journal-title":"EMBO J."},{"key":"BFncomms15754_CR42","doi-asserted-by":"publisher","first-page":"C641","DOI":"10.1152\/ajpcell.00222.2006","volume":"292","author":"O Kann","year":"2007","unstructured":"Kann, O. & Kovacs, R. Mitochondria and neuronal activity. Am. J. Physiol. Cell Physiol. 292, C641\u2013C657 (2007).","journal-title":"Am. J. Physiol. Cell Physiol."},{"key":"BFncomms15754_CR43","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1002\/iub.155","volume":"61","author":"A Szewczyk","year":"2009","unstructured":"Szewczyk, A., Jarmuszkiewicz, W. & Kunz, W. S. Mitochondrial potassium channels. IUBMB Life 61, 134\u2013143 (2009).","journal-title":"IUBMB Life"},{"key":"BFncomms15754_CR44","doi-asserted-by":"publisher","first-page":"1115","DOI":"10.1111\/j.1471-4159.2007.05210.x","volume":"105","author":"T Gaspar","year":"2008","unstructured":"Gaspar, T. et al. Delayed neuronal preconditioning by NS1619 is independent of calcium activated potassium channels. J. Neurochem. 105, 1115\u20131128 (2008).","journal-title":"J. Neurochem."},{"key":"BFncomms15754_CR45","doi-asserted-by":"publisher","first-page":"959","DOI":"10.1083\/jcb.200906083","volume":"187","author":"B Head","year":"2009","unstructured":"Head, B., Griparic, L., Amiri, M., Gandre-Babbe, S. & van der Bliek, A. M. Inducible proteolytic inactivation of OPA1 mediated by the OMA1 protease in mammalian cells. J. Cell Biol. 187, 959\u2013966 (2009).","journal-title":"J. Cell Biol."},{"key":"BFncomms15754_CR46","doi-asserted-by":"publisher","first-page":"919","DOI":"10.1083\/jcb.201308006","volume":"204","author":"R Anand","year":"2014","unstructured":"Anand, R. et al. The i-AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission. J. Cell Biol. 204, 919\u2013929 (2014).","journal-title":"J. Cell Biol."},{"key":"BFncomms15754_CR47","doi-asserted-by":"publisher","first-page":"16064","DOI":"10.1038\/srep16064","volume":"5","author":"C Ding","year":"2015","unstructured":"Ding, C. et al. Mitofilin and CHCHD6 physically interact with Sam50 to sustain cristae structure. Sci. Rep. 5, 16064 (2015).","journal-title":"Sci. Rep."},{"key":"BFncomms15754_CR48","doi-asserted-by":"publisher","first-page":"2918","DOI":"10.1074\/jbc.M110.171975","volume":"286","author":"M Darshi","year":"2011","unstructured":"Darshi, M. et al. ChChd3, an inner mitochondrial membrane protein, is essential for maintaining crista integrity and mitochondrial function. J. Biol. Chem. 286, 2918\u20132932 (2011).","journal-title":"J. Biol. Chem."},{"key":"BFncomms15754_CR49","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1023\/B:MCBI.0000009880.94044.49","volume":"256-257","author":"VP Skulachev","year":"2004","unstructured":"Skulachev, V. P. et al. Thread-grain transition of mitochondrial reticulum as a step of mitoptosis and apoptosis. Mol. Cell Biochem. 256-257, 341\u2013358 (2004).","journal-title":"Mol. Cell Biochem."},{"key":"BFncomms15754_CR50","doi-asserted-by":"publisher","first-page":"18792","DOI":"10.1074\/jbc.M400920200","volume":"279","author":"L Griparic","year":"2004","unstructured":"Griparic, L., van der Wel, N. N., Orozco, I. J., Peters, P. J. & van der Bliek, A. M. Loss of the intermembrane space protein Mgm1\/OPA1 induces swelling and localized constrictions along the lengths of mitochondria. J. Biol. Chem. 279, 18792\u201318798 (2004).","journal-title":"J. Biol. Chem."},{"key":"BFncomms15754_CR51","doi-asserted-by":"publisher","first-page":"16003","DOI":"10.1073\/pnas.1315784110","volume":"110","author":"F Stavru","year":"2013","unstructured":"Stavru, F., Palmer, A. E., Wang, C., Youle, R. J. & Cossart, P. Atypical mitochondrial fission upon bacterial infection. Proc. Natl Acad. Sci. USA 110, 16003\u201316008 (2013).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFncomms15754_CR52","doi-asserted-by":"publisher","first-page":"18","DOI":"10.1016\/j.ceca.2009.03.017","volume":"46","author":"T Brustovetsky","year":"2009","unstructured":"Brustovetsky, T., Li, V. & Brustovetsky, N. Stimulation of glutamate receptors in cultured hippocampal neurons causes Ca2+-dependent mitochondrial contraction. Cell Calcium 46, 18\u201329 (2009).","journal-title":"Cell Calcium"},{"key":"BFncomms15754_CR53","doi-asserted-by":"publisher","first-page":"783","DOI":"10.3389\/fpls.2015.00783","volume":"6","author":"EA Jaipargas","year":"2015","unstructured":"Jaipargas, E. A., Barton, K. A., Mathur, N. & Mathur, J. Mitochondrial pleomorphy in plant cells is driven by contiguous ER dynamics. Front. Plant. Sci. 6, 783 (2015).","journal-title":"Front. Plant. Sci."},{"key":"BFncomms15754_CR54","doi-asserted-by":"publisher","first-page":"3735","DOI":"10.1074\/jbc.M205760200","volume":"278","author":"TK Beuria","year":"2003","unstructured":"Beuria, T. K. et al. Glutamate-induced assembly of bacterial cell division protein FtsZ. J. Biol. Chem. 278, 3735\u20133741 (2003).","journal-title":"J. Biol. Chem."},{"key":"BFncomms15754_CR55","doi-asserted-by":"publisher","first-page":"20728","DOI":"10.1073\/pnas.0808953105","volume":"105","author":"M Saotome","year":"2008","unstructured":"Saotome, M. et al. Bidirectional Ca2+-dependent control of mitochondrial dynamics by the Miro GTPase. Proc. Natl Acad. Sci. USA 105, 20728\u201320733 (2008).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFncomms15754_CR56","doi-asserted-by":"publisher","first-page":"498","DOI":"10.1002\/jcp.21051","volume":"212","author":"JR Hom","year":"2007","unstructured":"Hom, J. R., Gewandter, J. S., Michael, L., Sheu, S. S. & Yoon, Y. Thapsigargin induces biphasic fragmentation of mitochondria through calcium-mediated mitochondrial fission and apoptosis. J. Cell Physiol. 212, 498\u2013508 (2007).","journal-title":"J. Cell Physiol."},{"key":"BFncomms15754_CR57","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1038\/ng.2851","volume":"46","author":"CV Logan","year":"2014","unstructured":"Logan, C. V. et al. Loss-of-function mutations in MICU1 cause a brain and muscle disorder linked to primary alterations in mitochondrial calcium signaling. Nat. Genet. 46, 188\u2013193 (2014).","journal-title":"Nat. Genet."},{"key":"BFncomms15754_CR58","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1083\/jcb.200802164","volume":"182","author":"XJ Han","year":"2008","unstructured":"Han, X. J. et al. CaM kinase I alpha-induced phosphorylation of Drp1 regulates mitochondrial morphology. J. Cell Biol. 182, 573\u2013585 (2008).","journal-title":"J. Cell Biol."},{"key":"BFncomms15754_CR59","doi-asserted-by":"publisher","first-page":"15716","DOI":"10.1523\/JNEUROSCI.3159-11.2011","volume":"31","author":"AS Dickey","year":"2011","unstructured":"Dickey, A. S. & Strack, S. PKA\/AKAP1 and PP2A\/Bbeta2 regulate neuronal morphogenesis via Drp1 phosphorylation and mitochondrial bioenergetics. J. Neurosci. 31, 15716\u201315726 (2011).","journal-title":"J. Neurosci."},{"key":"BFncomms15754_CR60","doi-asserted-by":"publisher","first-page":"15803","DOI":"10.1073\/pnas.0808249105","volume":"105","author":"GM Cereghetti","year":"2008","unstructured":"Cereghetti, G. M. et al. Dephosphorylation by calcineurin regulates translocation of Drp1 to mitochondria. Proc. Natl Acad. Sci. USA 105, 15803\u201315808 (2008).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFncomms15754_CR61","doi-asserted-by":"publisher","first-page":"E2595","DOI":"10.1073\/pnas.1504837112","volume":"112","author":"X Shao","year":"2015","unstructured":"Shao, X., Li, Q., Mogilner, A., Bershadsky, A. D. & Shivashankar, G. V. Mechanical stimulation induces formin-dependent assembly of a perinuclear actin rim. Proc. Natl Acad. Sci. USA 112, E2595\u2013E2601 (2015).","journal-title":"Proc. Natl Acad. Sci. USA"},{"key":"BFncomms15754_CR62","doi-asserted-by":"publisher","first-page":"1561","DOI":"10.1038\/cdd.2011.13","volume":"18","author":"X Liu","year":"2011","unstructured":"Liu, X. & Hajnoczky, G. Altered fusion dynamics underlie unique morphological changes in mitochondria during hypoxia-reoxygenation stress. Cell Death Differ. 18, 1561\u20131572 (2011).","journal-title":"Cell Death Differ."},{"key":"BFncomms15754_CR63","doi-asserted-by":"publisher","first-page":"2588","DOI":"10.1242\/jcs.026625","volume":"121","author":"S Tamai","year":"2008","unstructured":"Tamai, S. et al. Characterization of the mitochondrial protein LETM1, which maintains the mitochondrial tubular shapes and interacts with the AAA-ATPase BCS1L. J. Cell Sci. 121, 2588\u20132600 (2008).","journal-title":"J. Cell Sci."},{"key":"BFncomms15754_CR64","doi-asserted-by":"publisher","first-page":"50","DOI":"10.1016\/j.micron.2013.01.005","volume":"47","author":"JS Lee","year":"2013","unstructured":"Lee, J. S. et al. Aquaporin-assisted and ER-mediated mitochondrial fission: a hypothesis. Micron 47, 50\u201358 (2013).","journal-title":"Micron"},{"key":"BFncomms15754_CR65","doi-asserted-by":"publisher","first-page":"1029","DOI":"10.1126\/science.1074360","volume":"298","author":"W Xu","year":"2002","unstructured":"Xu, W. et al. Cytoprotective role of Ca2+- activated K+ channels in the cardiac inner mitochondrial membrane. Science 298, 1029\u20131033 (2002).","journal-title":"Science"},{"key":"BFncomms15754_CR66","doi-asserted-by":"publisher","first-page":"1927","DOI":"10.1038\/emboj.2013.124","volume":"32","author":"J Santo-Domingo","year":"2013","unstructured":"Santo-Domingo, J., Giacomello, M., Poburko, D., Scorrano, L. & Demaurex, N. OPA1 promotes pH flashes that spread between contiguous mitochondria without matrix protein exchange. EMBO J. 32, 1927\u20131940 (2013).","journal-title":"EMBO J."},{"key":"BFncomms15754_CR67","doi-asserted-by":"publisher","first-page":"749","DOI":"10.1083\/jcb.200704110","volume":"178","author":"Z Song","year":"2007","unstructured":"Song, Z., Chen, H., Fiket, M., Alexander, C. & Chan, D. C. OPA1 processing controls mitochondrial fusion and is regulated by mRNA splicing, membrane potential, and Yme1L. J. Cell Biol. 178, 749\u2013755 (2007).","journal-title":"J. Cell Biol."},{"key":"BFncomms15754_CR68","doi-asserted-by":"publisher","first-page":"630","DOI":"10.1016\/j.cmet.2014.03.011","volume":"19","author":"P Mishra","year":"2014","unstructured":"Mishra, P., Carelli, V., Manfredi, G. & Chan, D. C. Proteolytic cleavage of Opa1 stimulates mitochondrial inner membrane fusion and couples fusion to oxidative phosphorylation. Cell Metab. 19, 630\u2013641 (2014).","journal-title":"Cell Metab."},{"key":"BFncomms15754_CR69","doi-asserted-by":"publisher","first-page":"3309","DOI":"10.1002\/1873-3468.12384","volume":"590","author":"M Barrera","year":"2016","unstructured":"Barrera, M., Koob, S., Dikov, D., Vogel, F. & Reichert, A. S. OPA1 functionally interacts with MIC60 but is dispensable for crista junction formation. FEBS Lett. 590, 3309\u20133322 (2016).","journal-title":"FEBS Lett."},{"key":"BFncomms15754_CR70","doi-asserted-by":"publisher","first-page":"177","DOI":"10.1016\/j.cell.2006.06.025","volume":"126","author":"C Frezza","year":"2006","unstructured":"Frezza, C. et al. OPA1 controls apoptotic cristae remodeling independently from mitochondrial fusion. Cell 126, 177\u2013189 (2006).","journal-title":"Cell"},{"key":"BFncomms15754_CR71","doi-asserted-by":"publisher","first-page":"e25199","DOI":"10.1371\/journal.pone.0025199","volume":"6","author":"L Fulop","year":"2011","unstructured":"Fulop, L., Szanda, G., Enyedi, B., Varnai, P. & Spat, A. The effect of OPA1 on mitochondrial Ca(2)(+) signaling. PLoS ONE 6, e25199 (2011).","journal-title":"PLoS ONE"}],"container-title":["Nature Communications"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/ncomms15754.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/ncomms15754","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/doifinder\/10.1038\/ncomms15754","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/www.nature.com\/articles\/ncomms15754.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,12,22]],"date-time":"2022-12-22T20:14:51Z","timestamp":1671740091000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/ncomms15754"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,9]]},"references-count":71,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2017,8,30]]}},"alternative-id":["BFncomms15754"],"URL":"https:\/\/doi.org\/10.1038\/ncomms15754","relation":{},"ISSN":["2041-1723"],"issn-type":[{"value":"2041-1723","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,9]]},"assertion":[{"value":"14 June 2016","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"26 April 2017","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"9 June 2017","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}],"article-number":"15754"}}