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Critical for its pathogenesis is the ability of the phagocytosed bacteria to escape into the cell cytosol. For this, the bacteria use a non-canonical type VI secretion system (T6SS) encoded on the <jats:italic>Francisella<\/jats:italic> pathogenicity island (FPI). Here we show that in <jats:italic>F. novicida<\/jats:italic> T6SS assembly initiates at the bacterial poles both <jats:italic>in vitro<\/jats:italic> and within infected macrophages. T6SS dynamics and function depends on the general purpose ClpB unfoldase, which specifically colocalizes with contracted sheaths and is required for their disassembly. T6SS assembly depends on <jats:italic>iglF<\/jats:italic>, <jats:italic>iglG<\/jats:italic>, <jats:italic>iglI<\/jats:italic> and <jats:italic>iglJ<\/jats:italic>, whereas <jats:italic>pdpC<\/jats:italic>, <jats:italic>pdpD<\/jats:italic>, <jats:italic>pdpE<\/jats:italic> and <jats:italic>anmK<\/jats:italic> are dispensable. Importantly, strains lacking <jats:italic>pdpC<\/jats:italic> and <jats:italic>pdpD<\/jats:italic> are unable to escape from phagosome, activate AIM2 inflammasome or cause disease in mice. This suggests that PdpC and PdpD are T6SS effectors involved in phagosome rupture.<\/jats:p>","DOI":"10.1038\/ncomms15853","type":"journal-article","created":{"date-parts":[[2017,6,16]],"date-time":"2017-06-16T11:21:06Z","timestamp":1497612066000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":89,"title":["Francisella requires dynamic type VI secretion system and ClpB to deliver effectors for phagosomal escape"],"prefix":"10.1038","volume":"8","author":[{"given":"Maj","family":"Brodmann","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roland F.","family":"Dreier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Petr","family":"Broz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5414-2088","authenticated-orcid":false,"given":"Marek","family":"Basler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,6,16]]},"reference":[{"key":"BFncomms15853_CR1","doi-asserted-by":"publisher","first-page":"967","DOI":"10.1038\/nrmicro1045","volume":"2","author":"PCF Oyston","year":"2004","unstructured":"Oyston, P. 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