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The mechanism for epicardial gene (re-)activation remains elusive. Here we reveal that BRG1, the essential ATPase subunit of the SWI\/SNF chromatin\u2013remodelling complex, is required for expression of Wilms\u2019 tumour 1 (<jats:italic>Wt1<\/jats:italic>), fetal EPDC activation and subsequent differentiation into coronary smooth muscle, and restores <jats:italic>Wt1<\/jats:italic> activity upon MI. BRG1 physically interacts with T\u03b24 and is recruited by CCAAT\/enhancer-binding protein \u03b2 (C\/EBP\u03b2) to discrete regulatory elements in the <jats:italic>Wt1<\/jats:italic> locus. BRG1-T\u03b24 co-operative binding promotes optimal transcription of <jats:italic>Wt1<\/jats:italic> as the master regulator of embryonic EPDCs. Moreover, chromatin immunoprecipitation-sequencing reveals BRG1 binding at further key loci suggesting SWI\/SNF activity across the fetal epicardial gene programme. These findings reveal essential functions for chromatin\u2013remodelling in the activation of EPDCs during cardiovascular development and repair.<\/jats:p>","DOI":"10.1038\/ncomms16034","type":"journal-article","created":{"date-parts":[[2017,7,24]],"date-time":"2017-07-24T10:20:46Z","timestamp":1500891646000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":85,"title":["BRG1-SWI\/SNF-dependent regulation of the Wt1 transcriptional landscape mediates epicardial activity during heart development and disease"],"prefix":"10.1038","volume":"8","author":[{"given":"Joaquim Miguel","family":"Vieira","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Howard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Villa del Campo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sveva","family":"Bollini","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karina N.","family":"Dub\u00e9","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Megan","family":"Masters","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damien N.","family":"Barnette","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mala","family":"Rohling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura E.","family":"Hankins","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daria","family":"Gavriouchkina","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Metzger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pierre","family":"Chambon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatjana","family":"Sauka-Spengler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3623-600X","authenticated-orcid":false,"given":"Benjamin","family":"Davies","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul R.","family":"Riley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,7,24]]},"reference":[{"key":"BFncomms16034_CR1","doi-asserted-by":"publisher","first-page":"10165","DOI":"10.1073\/pnas.1302209110","volume":"110","author":"MY Tolstorukov","year":"2013","unstructured":"Tolstorukov, M. 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