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The hepatointestinal form of this debilitating disease results from a chronic infection with <jats:italic>Schistosoma mansoni<\/jats:italic> or <jats:italic>Schistosoma japonicum<\/jats:italic>. No vaccine is available to prevent schistosomiasis, and treatment relies predominantly on the use of a single drug, praziquantel. In spite of considerable research effort over the years, very little is known about the complex <jats:italic>in vivo<\/jats:italic> events that lead to granuloma formation and other pathological changes during infection. Here we use, for the first time, a lentivirus-based transduction system to deliver microRNA-adapted short hairpin RNAs (shRNAmirs) into the parasite to silence and explore selected protein-encoding genes of <jats:italic>S. mansoni<\/jats:italic> implicated in the disease process. This gene-silencing system has potential to be used for functional genomic\u2013phenomic studies of a range of socioeconomically important pathogens.<\/jats:p>","DOI":"10.1038\/ncomms6375","type":"journal-article","created":{"date-parts":[[2014,11,17]],"date-time":"2014-11-17T11:37:52Z","timestamp":1416224272000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":68,"title":["Omega-1 knockdown in Schistosoma mansoni eggs by lentivirus transduction reduces granuloma size in vivo"],"prefix":"10.1038","volume":"5","author":[{"given":"Jana","family":"Hagen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil D.","family":"Young","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison L.","family":"Every","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles N.","family":"Pagel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Corinna","family":"Schnoeller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Pierre Y.","family":"Scheerlinck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin B.","family":"Gasser","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernd H.","family":"Kalinna","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2014,11,17]]},"reference":[{"key":"BFncomms6375_CR1","doi-asserted-by":"publisher","first-page":"1593","DOI":"10.1017\/S0031182009990552","volume":"136","author":"D Rollinson","year":"2009","unstructured":"Rollinson, D. 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