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Here we focus on a set of intergenic lncRNAs in fission yeast. Deleting one of these lncRNAs exhibited a clear phenotype: drug sensitivity. Detailed analyses of the affected locus revealed that transcription of the <jats:italic>nc-tgp1<\/jats:italic> lncRNA regulates drug tolerance by repressing the adjacent phosphate-responsive permease gene <jats:italic>transporter for glycerophosphodiester 1<\/jats:italic> (<jats:italic>tgp1<\/jats:italic><jats:sup>+<\/jats:sup>). We demonstrate that the act of transcribing <jats:italic>nc-tgp1<\/jats:italic> over the <jats:italic>tgp1<\/jats:italic><jats:sup>+<\/jats:sup> promoter increases nucleosome density, prevents transcription factor access and thus represses <jats:italic>tgp1<\/jats:italic><jats:sup>+<\/jats:sup> without the need for RNA interference or heterochromatin components. We therefore conclude that <jats:italic>tgp1<\/jats:italic><jats:sup>+<\/jats:sup> is regulated by transcriptional interference. Accordingly, decreased <jats:italic>nc-tgp1<\/jats:italic> transcription permits <jats:italic>tgp1<\/jats:italic><jats:sup>+<\/jats:sup> expression upon phosphate starvation. Furthermore, <jats:italic>nc-tgp1<\/jats:italic> loss induces <jats:italic>tgp1<\/jats:italic><jats:sup>+<\/jats:sup> even in repressive conditions. Notably, drug sensitivity results directly from <jats:italic>tgp1<\/jats:italic><jats:sup>+<\/jats:sup> expression in the absence of the <jats:italic>nc-tgp1<\/jats:italic> RNA. Thus, transcription of an lncRNA governs drug tolerance in fission yeast.<\/jats:p>","DOI":"10.1038\/ncomms6576","type":"journal-article","created":{"date-parts":[[2014,11,27]],"date-time":"2014-11-27T13:26:08Z","timestamp":1417094768000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":99,"title":["Long non-coding RNA-mediated transcriptional interference of a permease gene confers drug tolerance in fission yeast"],"prefix":"10.1038","volume":"5","author":[{"given":"Ryan","family":"Ard","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pin","family":"Tong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robin C.","family":"Allshire","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2014,11,27]]},"reference":[{"key":"BFncomms6576_CR1","doi-asserted-by":"publisher","first-page":"223","DOI":"10.1038\/nature07672","volume":"458","author":"Guttman","year":"2009","unstructured":"Guttman, et al. 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