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Here we present the results of an unbiased, comprehensive genome-wide survey of the range of germline mutations induced in laboratory mice after parental exposure to ionizing radiation and show irradiation markedly alters the frequency and spectrum of <jats:italic>de novo<\/jats:italic> mutations. Here we show that the frequency of <jats:italic>de novo<\/jats:italic> copy number variants (CNVs) and insertion\/deletion events (indels) is significantly elevated in offspring of exposed fathers. We also show that the spectrum of induced <jats:italic>de novo<\/jats:italic> single-nucleotide variants (SNVs) is strikingly different; with clustered mutations being significantly over-represented in the offspring of irradiated males. Our study highlights the specific classes of radiation-induced DNA lesions that evade repair and result in germline mutation and paves the way for similarly comprehensive characterizations of other germline mutagens.<\/jats:p>","DOI":"10.1038\/ncomms7684","type":"journal-article","created":{"date-parts":[[2015,3,26]],"date-time":"2015-03-26T10:55:01Z","timestamp":1427367301000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":148,"title":["The genome-wide effects of ionizing radiation on mutation induction in the mammalian germline"],"prefix":"10.1038","volume":"6","author":[{"given":"Adeolu B.","family":"Adewoye","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah J.","family":"Lindsay","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuri E.","family":"Dubrova","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew E.","family":"Hurles","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,3,26]]},"reference":[{"key":"BFncomms7684_CR1","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1007\/BF01210408","volume":"29","author":"M Frankenberg-Schwager","year":"1990","unstructured":"Frankenberg-Schwager, M. 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