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Conflicting models propose that stereocilia F-actin cores are either continually renewed every 24\u201348\u2009h via a treadmill or are stable, exceptionally long-lived structures. Here to distinguish between these models, we perform an unbiased survey of stereocilia actin dynamics in more than 500 utricle hair cells. Live-imaging EGFP-\u03b2-actin or dendra2-\u03b2-actin reveal stable F-actin cores with turnover and elongation restricted to stereocilia tips. Fixed-cell microscopy of wild-type and mutant \u03b2-actin demonstrates that incorporation of actin monomers into filaments is required for localization to stereocilia tips. Multi-isotope imaging mass spectrometry and live imaging of single differentiating hair cells capture stereociliogenesis and explain uniform incorporation of\n                    <jats:sup>15<\/jats:sup>\n                    N-labelled protein and EGFP-\u03b2-actin into nascent stereocilia. Collectively, our analyses support a model in which stereocilia actin cores are stable structures that incorporate new F-actin only at the distal tips.\n                  <\/jats:p>","DOI":"10.1038\/ncomms7873","type":"journal-article","created":{"date-parts":[[2015,4,21]],"date-time":"2015-04-21T11:10:27Z","timestamp":1429614627000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":83,"title":["Live-cell imaging of actin dynamics reveals mechanisms of stereocilia length regulation in the inner ear"],"prefix":"10.1038","volume":"6","author":[{"given":"Meghan C.","family":"Drummond","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melanie","family":"Barzik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan E.","family":"Bird","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duan-Sun","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claude P.","family":"Lechene","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David P.","family":"Corey","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa L.","family":"Cunningham","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas B.","family":"Friedman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,4,21]]},"reference":[{"key":"BFncomms7873_CR1","doi-asserted-by":"publisher","first-page":"597","DOI":"10.1387\/ijdb.072392gn","volume":"51","author":"GD Nayak","year":"2007","unstructured":"Nayak, G. 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