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Based on studies with the V-ATPase inhibitor BafilomycinA1, lysosomal acidification is also thought to be required for fusion with incoming vesicles from the autophagic and endocytic pathways. Here we show that loss of V-ATPase subunits in the\n                    <jats:italic>Drosophila<\/jats:italic>\n                    fat body causes an accumulation of non-functional lysosomes, leading to a block in autophagic flux. However, V-ATPase-deficient lysosomes remain competent to fuse with autophagosomes and endosomes, resulting in a time-dependent formation of giant autolysosomes. In contrast, BafilomycinA1 prevents autophagosome\u2013lysosome fusion in these cells, and this defect is phenocopied by depletion of the Ca\n                    <jats:sup>2+<\/jats:sup>\n                    pump SERCA, a secondary target of this drug. Moreover, activation of SERCA promotes fusion in a BafilomycinA1-sensitive manner. Collectively, our results indicate that lysosomal acidification is not a prerequisite for fusion, and that BafilomycinA1 inhibits fusion independent of its effect on lysosomal pH.\n                  <\/jats:p>","DOI":"10.1038\/ncomms8007","type":"journal-article","created":{"date-parts":[[2015,5,13]],"date-time":"2015-05-13T06:02:48Z","timestamp":1431496968000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":422,"title":["Autophagosome\u2013lysosome fusion is independent of V-ATPase-mediated acidification"],"prefix":"10.1038","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4220-7272","authenticated-orcid":false,"given":"Caroline","family":"Mauvezin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e9ter","family":"Nagy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G\u00e1bor","family":"Juh\u00e1sz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas P.","family":"Neufeld","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,5,11]]},"reference":[{"key":"BFncomms8007_CR1","doi-asserted-by":"publisher","first-page":"460","DOI":"10.1016\/j.biocel.2011.01.006","volume":"43","author":"I Beau","year":"2011","unstructured":"Beau, I., Mehrpour, M. & Codogno, P. 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