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Alternatively, where these methods are lacking, the construction of pre-characterized libraries of mutants can be extremely valuable. However, this can be complex, expensive and time consuming. Here, we describe a robust, easy to implement parallel sequencing-based method (Cartesian Pooling-Coordinate Sequencing or CP-CSeq) that reports both on the identity as well as on the location of sequence-tagged biological entities in well-plate archived clone collections. We demonstrate this approach using a transposon insertion mutant library of the <jats:italic>Mycobacterium bovis<\/jats:italic> BCG vaccine strain, providing the largest resource of mutants in any strain of the <jats:italic>M. tuberculosis<\/jats:italic> complex. The method is applicable to any entity for which sequence-tagged identification is possible.<\/jats:p>","DOI":"10.1038\/ncomms8106","type":"journal-article","created":{"date-parts":[[2015,5,18]],"date-time":"2015-05-18T09:51:00Z","timestamp":1431942660000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Characterization of genome-wide ordered sequence-tagged Mycobacterium mutant libraries by Cartesian Pooling-Coordinate Sequencing"],"prefix":"10.1038","volume":"6","author":[{"given":"Kristof","family":"Vandewalle","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nele","family":"Festjens","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evelyn","family":"Plets","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marnik","family":"Vuylsteke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvan","family":"Saeys","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nico","family":"Callewaert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,5,11]]},"reference":[{"key":"BFncomms8106_CR1","doi-asserted-by":"publisher","first-page":"143","DOI":"10.1016\/j.cell.2010.11.052","volume":"144","author":"RJ Nichols","year":"2011","unstructured":"Nichols, R. 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