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Here we demonstrate that ZnuD is required for efficient systemic infections by the causative agent of bacterial meningitis, <jats:italic>Neisseria meningitidis<\/jats:italic>, in a mouse model. We also combine X-ray crystallography and molecular dynamics simulations to gain insight into the mechanism of zinc recognition and transport across the bacterial outer-membrane by ZnuD. Because ZnuD is also considered a promising vaccine candidate against <jats:italic>N. meningitidis<\/jats:italic>, we use several ZnuD structural intermediates to map potential antigenic epitopes, and propose a mechanism by which ZnuD can maintain high sequence conservation yet avoid immune recognition by altering the conformation of surface-exposed loops.<\/jats:p>","DOI":"10.1038\/ncomms8996","type":"journal-article","created":{"date-parts":[[2015,8,18]],"date-time":"2015-08-18T09:42:18Z","timestamp":1439890938000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":73,"title":["The molecular mechanism of Zinc acquisition by the neisserial outer-membrane transporter ZnuD"],"prefix":"10.1038","volume":"6","author":[{"given":"Charles","family":"Calmettes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher","family":"Ing","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carolyn M.","family":"Buckwalter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Majida","family":"El Bakkouri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Chieh-Lin Lai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anastassia","family":"Pogoutse","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1477-3616","authenticated-orcid":false,"given":"Scott D.","family":"Gray-Owen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R\u00e9gis","family":"Pom\u00e8s","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trevor F.","family":"Moraes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,8,18]]},"reference":[{"key":"BFncomms8996_CR1","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1038\/nrmicro2836","volume":"10","author":"MI Hood","year":"2012","unstructured":"Hood, M. 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