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Numerous roles for PICH have been proposed from protein depletion experiments, but a consensus has failed to emerge. Here, we report that deletion of\n                    <jats:italic>PICH<\/jats:italic>\n                    in avian cells causes chromosome structural abnormalities, and hypersensitivity to an inhibitor of Topoisomerase II (Topo II), ICRF-193. ICRF-193-treated\n                    <jats:italic>PICH<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    cells undergo sister chromatid non-disjunction in anaphase, and frequently abort cytokinesis. PICH co-localizes with Topo II\u03b1 on UFBs and at the ribosomal DNA locus, and the timely resolution of both structures depends on the ATPase activity of PICH. Purified PICH protein strongly stimulates the catalytic activity of Topo II\n                    <jats:italic>in vitro<\/jats:italic>\n                    . Consistent with this, a human\n                    <jats:italic>PICH<\/jats:italic>\n                    <jats:sup>\u2212\/\u2212<\/jats:sup>\n                    cell line exhibits chromosome instability and chromosome condensation and decatenation defects similar to those of ICRF-193-treated cells. We propose that PICH and Topo II cooperate to prevent chromosome missegregation events in mitosis.\n                  <\/jats:p>","DOI":"10.1038\/ncomms9962","type":"journal-article","created":{"date-parts":[[2015,12,8]],"date-time":"2015-12-08T05:11:10Z","timestamp":1449551470000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":119,"title":["PICH promotes sister chromatid disjunction and co-operates with topoisomerase II in mitosis"],"prefix":"10.1038","volume":"6","author":[{"given":"Christian F.","family":"Nielsen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana","family":"Huttner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1858-3999","authenticated-orcid":false,"given":"Anna H.","family":"Bizard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seiki","family":"Hirano","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian-Neng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7402-2144","authenticated-orcid":false,"given":"Timea","family":"Palmai-Pallag","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Victoria A.","family":"Bjerregaard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erich A.","family":"Nigg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lily Hui-Ching","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian D.","family":"Hickson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2015,12,8]]},"reference":[{"key":"BFncomms9962_CR1","doi-asserted-by":"publisher","first-page":"492","DOI":"10.1038\/35019529","volume":"2","author":"T Tanaka","year":"2000","unstructured":"Tanaka, T., Fuchs, J., Loidl, J. & Nasmyth, K. 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