{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,11]],"date-time":"2026-10-11T21:24:26Z","timestamp":1791753866308,"version":"4.3.4"},"reference-count":54,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T00:00:00Z","timestamp":1401580800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Nat Med"],"published-print":{"date-parts":[[2014,8]]},"DOI":"10.1038\/nm.3600","type":"journal-article","created":{"date-parts":[[2014,6,1]],"date-time":"2014-06-01T17:13:20Z","timestamp":1401642800000},"page":"897-903","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":629,"title":["Tumorigenicity and genetic profiling of circulating tumor cells in small-cell lung cancer"],"prefix":"10.1038","volume":"20","author":[{"given":"Cassandra L","family":"Hodgkinson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J","family":"Morrow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yaoyong","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert L","family":"Metcalf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dominic G","family":"Rothwell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Trapani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Radoslaw","family":"Polanski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah J","family":"Burt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathryn L","family":"Simpson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Morris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stuart D","family":"Pepper","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Nonaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alastair","family":"Greystoke","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Kelly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Becky","family":"Bola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew G","family":"Krebs","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenny","family":"Antonello","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mahmood","family":"Ayub","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Suzanne","family":"Faulkner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynsey","family":"Priest","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Carter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catriona","family":"Tate","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Crispin J","family":"Miller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fiona","family":"Blackhall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ged","family":"Brady","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline","family":"Dive","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2014,6,1]]},"reference":[{"key":"BFnm3600_CR1","doi-asserted-by":"publisher","first-page":"1225","DOI":"10.1001\/jama.1976.03260380019019","volume":"235","author":"LH Einhorn","year":"1976","unstructured":"Einhorn, L.H., Fee, W.H., Farber, M.O., Livingston, R.B. & Gottlieb, J.A. Improved chemotherapy for small-cell undifferentiated lung cancer. J. Am. Med. Assoc. 235, 1225\u20131229 (1976).","journal-title":"J. Am. Med. Assoc."},{"key":"BFnm3600_CR2","doi-asserted-by":"publisher","first-page":"1471","DOI":"10.1200\/JCO.1985.3.11.1471","volume":"3","author":"WK Evans","year":"1985","unstructured":"Evans, W.K. et al. VP-16 and cisplatin as first-line therapy for small-cell lung cancer. J. Clin. Oncol. 3, 1471\u20131477 (1985).","journal-title":"J. Clin. Oncol."},{"key":"BFnm3600_CR3","first-page":"1593","volume":"63","author":"JS Sierocki","year":"1979","unstructured":"Sierocki, J.S. et al. cis-Dichlorodiammineplatinum(II) and VP-16\u2013213: an active induction regimen for small cell carcinoma of the lung. Cancer Treat. Rep. 63, 1593\u20131597 (1979).","journal-title":"Cancer Treat. Rep."},{"key":"BFnm3600_CR4","first-page":"3502","volume":"40","author":"AF Gazdar","year":"1980","unstructured":"Gazdar, A.F. et al. Establishment of continuous, clonable cultures of small-cell carcinoma of lung which have amine precursor uptake and decarboxylation cell properties. Cancer Res. 40, 3502\u20133507 (1980).","journal-title":"Cancer Res."},{"key":"BFnm3600_CR5","first-page":"505","volume":"62","author":"S Oboshi","year":"1971","unstructured":"Oboshi, S., Tsugawa, S., Seido, T., Shimosato, Y. & Koide, T. A new floating cell line derived from human pulmonary carcinoma of oat cell type. Gann 62, 505\u2013514 (1971).","journal-title":"Gann"},{"key":"BFnm3600_CR6","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1038\/nature08629","volume":"463","author":"ED Pleasance","year":"2010","unstructured":"Pleasance, E.D. et al. A small-cell lung cancer genome with complex signatures of tobacco exposure. Nature 463, 184\u2013190 (2010).","journal-title":"Nature"},{"key":"BFnm3600_CR7","doi-asserted-by":"publisher","first-page":"385","DOI":"10.1007\/978-1-4614-6176-0_18","volume":"779","author":"M Joshi","year":"2013","unstructured":"Joshi, M., Ayoola, A. & Belani, C.P. Small-cell lung cancer: an update on targeted therapies. Adv. Exp. Med. Biol. 779, 385\u2013404 (2013).","journal-title":"Adv. Exp. Med. Biol."},{"key":"BFnm3600_CR8","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1038\/nrclinonc.2011.90","volume":"8","author":"WN William Jr.","year":"2011","unstructured":"William, W.N. Jr. & Glisson, B.S. Novel strategies for the treatment of small-cell lung carcinoma. Nat. Rev. Clin. Oncol. 8, 611\u2013619 (2011).","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"BFnm3600_CR9","doi-asserted-by":"publisher","first-page":"181","DOI":"10.1016\/S1535-6108(03)00220-4","volume":"4","author":"R Meuwissen","year":"2003","unstructured":"Meuwissen, R. et al. Induction of small cell lung cancer by somatic inactivation of both Trp53 and Rb1 in a conditional mouse model. Cancer Cell 4, 181\u2013189 (2003).","journal-title":"Cancer Cell"},{"key":"BFnm3600_CR10","doi-asserted-by":"publisher","first-page":"165","DOI":"10.1016\/j.molonc.2013.02.010","volume":"7","author":"MC Kwon","year":"2013","unstructured":"Kwon, M.C. & Berns, A. Mouse models for lung cancer. Mol. Oncol. 7, 165\u2013177 (2013).","journal-title":"Mol. Oncol."},{"key":"BFnm3600_CR11","doi-asserted-by":"publisher","first-page":"3364","DOI":"10.1158\/0008-5472.CAN-08-4210","volume":"69","author":"VC Daniel","year":"2009","unstructured":"Daniel, V.C. et al. A primary xenograft model of small-cell lung cancer reveals irreversible changes in gene expression imposed by culture in vitro. Cancer Res. 69, 3364\u20133373 (2009).","journal-title":"Cancer Res."},{"key":"BFnm3600_CR12","doi-asserted-by":"publisher","first-page":"2023","DOI":"10.1093\/jnci\/85.24.2023","volume":"85","author":"MF Poupon","year":"1993","unstructured":"Poupon, M.F. et al. Response of small-cell lung cancer xenografts to chemotherapy: multidrug resistance and direct clinical correlates. J. Natl. Cancer Inst. 85, 2023\u20132029 (1993).","journal-title":"J. Natl. Cancer Inst."},{"key":"BFnm3600_CR13","first-page":"502","volume":"34","author":"RD Davenport","year":"1990","unstructured":"Davenport, R.D. Diagnostic value of crush artifact in cytologic specimens. Occurrence in small cell carcinoma of the lung. Acta Cytol. 34, 502\u2013504 (1990).","journal-title":"Acta Cytol."},{"key":"BFnm3600_CR14","doi-asserted-by":"publisher","first-page":"1104","DOI":"10.1038\/ng.2396","volume":"44","author":"M Peifer","year":"2012","unstructured":"Peifer, M. et al. Integrative genome analyses identify key somatic driver mutations of small-cell lung cancer. Nat. Genet. 44, 1104\u20131110 (2012).","journal-title":"Nat. Genet."},{"key":"BFnm3600_CR15","doi-asserted-by":"publisher","first-page":"1111","DOI":"10.1038\/ng.2405","volume":"44","author":"CM Rudin","year":"2012","unstructured":"Rudin, C.M. et al. Comprehensive genomic analysis identifies SOX2 as a frequently amplified gene in small-cell lung cancer. Nat. Genet. 44, 1111\u20131116 (2012).","journal-title":"Nat. Genet."},{"key":"BFnm3600_CR16","doi-asserted-by":"publisher","first-page":"781","DOI":"10.1056\/NEJMoa040766","volume":"351","author":"M Cristofanilli","year":"2004","unstructured":"Cristofanilli, M. et al. Circulating tumor cells, disease progression, and survival in metastatic breast cancer. N. Engl. J. Med. 351, 781\u2013791 (2004).","journal-title":"N. Engl. J. Med."},{"key":"BFnm3600_CR17","doi-asserted-by":"publisher","first-page":"6302","DOI":"10.1158\/1078-0432.CCR-08-0872","volume":"14","author":"JS de Bono","year":"2008","unstructured":"de Bono, J.S. et al. Circulating tumor cells predict survival benefit from treatment in metastatic castration-resistant prostate cancer. Clin. Cancer Res. 14, 6302\u20136309 (2008).","journal-title":"Clin. Cancer Res."},{"key":"BFnm3600_CR18","doi-asserted-by":"publisher","first-page":"4218","DOI":"10.1158\/1078-0432.CCR-05-2821","volume":"12","author":"DF Hayes","year":"2006","unstructured":"Hayes, D.F. et al. Circulating tumor cells at each follow-up time point during therapy of metastatic breast cancer patients predict progression-free and overall survival. Clin. Cancer Res. 12, 4218\u20134224 (2006).","journal-title":"Clin. Cancer Res."},{"key":"BFnm3600_CR19","doi-asserted-by":"publisher","first-page":"525","DOI":"10.1200\/JCO.2010.33.3716","volume":"30","author":"JM Hou","year":"2012","unstructured":"Hou, J.M. et al. Clinical significance and molecular characteristics of circulating tumor cells and circulating tumor microemboli in patients with small-cell lung cancer. J. Clin. Oncol. 30, 525\u2013532 (2012).","journal-title":"J. Clin. Oncol."},{"key":"BFnm3600_CR20","doi-asserted-by":"publisher","first-page":"1556","DOI":"10.1200\/JCO.2010.28.7045","volume":"29","author":"MG Krebs","year":"2011","unstructured":"Krebs, M.G. et al. Evaluation and prognostic significance of circulating tumor cells in patients with non-small-cell lung cancer. J. Clin. Oncol. 29, 1556\u20131563 (2011).","journal-title":"J. Clin. Oncol."},{"key":"BFnm3600_CR21","doi-asserted-by":"publisher","first-page":"808","DOI":"10.2353\/ajpath.2009.090078","volume":"175","author":"JM Hou","year":"2009","unstructured":"Hou, J.M. et al. Evaluation of circulating tumor cells and serological cell death biomarkers in small cell lung cancer patients undergoing chemotherapy. Am. J. Pathol. 175, 808\u2013816 (2009).","journal-title":"Am. J. Pathol."},{"key":"BFnm3600_CR22","doi-asserted-by":"publisher","first-page":"1461","DOI":"10.1002\/1097-0142(19840401)53:7<1461::AID-CNCR2820530706>3.0.CO;2-N","volume":"53","author":"WK Evans","year":"1984","unstructured":"Evans, W.K. et al. VP-16 alone and in combination with cisplatin in previously treated patients with small cell lung cancer. Cancer 53, 1461\u20131466 (1984).","journal-title":"Cancer"},{"key":"BFnm3600_CR23","doi-asserted-by":"publisher","first-page":"4875","DOI":"10.1158\/0008-5472.CAN-12-2217","volume":"72","author":"C Swanton","year":"2012","unstructured":"Swanton, C. Intratumor heterogeneity: evolution through space and time. Cancer Res. 72, 4875\u20134882 (2012).","journal-title":"Cancer Res."},{"key":"BFnm3600_CR24","doi-asserted-by":"publisher","first-page":"356","DOI":"10.1002\/path.4214","volume":"230","author":"P Martinez","year":"2013","unstructured":"Martinez, P. et al. Parallel evolution of tumour subclones mimics diversity between tumours. J. Pathol. 230, 356\u2013364 (2013).","journal-title":"J. Pathol."},{"key":"BFnm3600_CR25","doi-asserted-by":"publisher","first-page":"189","DOI":"10.1007\/s12094-008-0181-1","volume":"10","author":"E Arriola","year":"2008","unstructured":"Arriola, E. et al. Genetic changes in small cell lung carcinoma. Clin. Transl. Oncol. 10, 189\u2013197 (2008).","journal-title":"Clin. Transl. Oncol."},{"key":"BFnm3600_CR26","first-page":"1713","volume":"5","author":"N Mori","year":"1990","unstructured":"Mori, N. et al. Variable mutations of the RB gene in small-cell lung carcinoma. Oncogene 5, 1713\u20131717 (1990).","journal-title":"Oncogene"},{"key":"BFnm3600_CR27","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/S0093-7754(01)90072-7","volume":"28","author":"II Wistuba","year":"2001","unstructured":"Wistuba, I.I. & Gazdar, A.F. & Minna, J.D. Molecular genetics of small cell lung carcinoma. Semin. Oncol. 28, 3\u201313 (2001).","journal-title":"Semin. Oncol."},{"key":"BFnm3600_CR28","doi-asserted-by":"publisher","first-page":"539","DOI":"10.1038\/nbt.2576","volume":"31","author":"I Baccelli","year":"2013","unstructured":"Baccelli, I. et al. Identification of a population of blood circulating tumor cells from breast cancer patients that initiates metastasis in a xenograft assay. Nat. Biotechnol. 31, 539\u2013544 (2013).","journal-title":"Nat. Biotechnol."},{"key":"BFnm3600_CR29","doi-asserted-by":"publisher","first-page":"6897","DOI":"10.1158\/1078-0432.CCR-04-0378","volume":"10","author":"WJ Allard","year":"2004","unstructured":"Allard, W.J. et al. Tumor cells circulate in the peripheral blood of all major carcinomas but not in healthy subjects or patients with nonmalignant diseases. Clin. Cancer Res. 10, 6897\u20136904 (2004).","journal-title":"Clin. Cancer Res."},{"key":"BFnm3600_CR30","first-page":"4033","volume":"60","author":"TG Pretlow","year":"2000","unstructured":"Pretlow, T.G. et al. Prostate cancer and other xenografts from cells in peripheral blood of patients. Cancer Res. 60, 4033\u20134036 (2000).","journal-title":"Cancer Res."},{"key":"BFnm3600_CR31","doi-asserted-by":"publisher","first-page":"21083","DOI":"10.1073\/pnas.1320659110","volume":"110","author":"X Ni","year":"2013","unstructured":"Ni, X. et al. Reproducible copy number variation patterns among single circulating tumor cells of lung cancer patients. Proc. Natl. Acad. Sci. USA 110, 21083\u201321088 (2013).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnm3600_CR32","doi-asserted-by":"publisher","first-page":"365","DOI":"10.1038\/nature12628","volume":"501","author":"CA Klein","year":"2013","unstructured":"Klein, C.A. Selection and adaptation during metastatic cancer progression. Nature 501, 365\u2013372 (2013).","journal-title":"Nature"},{"key":"BFnm3600_CR33","doi-asserted-by":"publisher","first-page":"252","DOI":"10.1373\/clinchem.2012.188557","volume":"59","author":"C Gasch","year":"2013","unstructured":"Gasch, C. et al. Heterogeneity of epidermal growth factor receptor status and mutations of KRAS\/PIK3CA in circulating tumor cells of patients with colorectal cancer. Clin. Chem. 59, 252\u2013260 (2013).","journal-title":"Clin. Chem."},{"key":"BFnm3600_CR34","doi-asserted-by":"publisher","first-page":"225","DOI":"10.1016\/j.canlet.2013.02.015","volume":"335","author":"F Fabbri","year":"2013","unstructured":"Fabbri, F. et al. Detection and recovery of circulating colon cancer cells using a dielectrophoresis-based device: KRAS mutation status in pure CTCs. Cancer Lett. 335, 225\u2013231 (2013).","journal-title":"Cancer Lett."},{"key":"BFnm3600_CR35","doi-asserted-by":"publisher","first-page":"2965","DOI":"10.1158\/0008-5472.CAN-12-4140","volume":"73","author":"E Heitzer","year":"2013","unstructured":"Heitzer, E. et al. Complex tumor genomes inferred from single circulating tumor cells by array-CGH and next-generation sequencing. Cancer Res. 73, 2965\u20132975 (2013).","journal-title":"Cancer Res."},{"key":"BFnm3600_CR36","doi-asserted-by":"publisher","first-page":"683","DOI":"10.1016\/S0140-6736(02)09838-0","volume":"360","author":"CA Klein","year":"2002","unstructured":"Klein, C.A. et al. Genetic heterogeneity of single disseminated tumour cells in minimal residual cancer. Lancet 360, 683\u2013689 (2002).","journal-title":"Lancet"},{"key":"BFnm3600_CR37","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1038\/nbt0402-387","volume":"20","author":"CA Klein","year":"2002","unstructured":"Klein, C.A. et al. Combined transcriptome and genome analysis of single micrometastatic cells. Nat. Biotechnol. 20, 387\u2013392 (2002).","journal-title":"Nat. Biotechnol."},{"key":"BFnm3600_CR38","doi-asserted-by":"publisher","first-page":"e45","DOI":"10.1200\/JCO.2011.36.9678","volume":"30","author":"MP Morelli","year":"2012","unstructured":"Morelli, M.P. et al. Prioritizing phase I treatment options through preclinical testing on personalized tumorgraft. J. Clin. Oncol. 30, e45\u2013e48 (2012).","journal-title":"J. Clin. Oncol."},{"key":"BFnm3600_CR39","doi-asserted-by":"publisher","first-page":"98","DOI":"10.1196\/annals.1448.020","volume":"1137","author":"RE Board","year":"2008","unstructured":"Board, R.E. et al. Isolation and extraction of circulating tumor DNA from patients with small cell lung cancer. Ann. NY Acad. Sci. 1137, 98\u2013107 (2008).","journal-title":"Ann. NY Acad. Sci."},{"key":"BFnm3600_CR40","doi-asserted-by":"publisher","first-page":"224ra224","DOI":"10.1126\/scitranslmed.3007094","volume":"6","author":"C Bettegowda","year":"2014","unstructured":"Bettegowda, C. et al. Detection of circulating tumor DNA in early- and late-stage human malignancies. Sci .Transl. Med. 6, 224ra224 (2014).","journal-title":"Sci .Transl. Med."},{"key":"BFnm3600_CR41","doi-asserted-by":"publisher","first-page":"1199","DOI":"10.1056\/NEJMoa1213261","volume":"368","author":"SJ Dawson","year":"2013","unstructured":"Dawson, S.J. et al. Analysis of circulating tumor DNA to monitor metastatic breast cancer. N. Engl. J. Med. 368, 1199\u20131209 (2013).","journal-title":"N. Engl. J. Med."},{"key":"BFnm3600_CR42","doi-asserted-by":"publisher","first-page":"129","DOI":"10.1038\/nrclinonc.2013.253","volume":"11","author":"MG Krebs","year":"2014","unstructured":"Krebs, M.G. et al. Molecular analysis of circulating tumour cells\u2014biology and biomarkers. Nat. Rev. Clin. Oncol. 11, 129\u2013144 (2014).","journal-title":"Nat. Rev. Clin. Oncol."},{"key":"BFnm3600_CR43","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1086\/514845","volume":"61","author":"DR Lohmann","year":"1997","unstructured":"Lohmann, D.R. et al. Constitutional RB1-gene mutations in patients with isolated unilateral retinoblastoma. Am. J. Hum. Genet. 61, 282\u2013294 (1997).","journal-title":"Am. J. Hum. Genet."},{"key":"BFnm3600_CR44","doi-asserted-by":"publisher","first-page":"475","DOI":"10.1136\/jmg.32.6.475","volume":"32","author":"I Szijan","year":"1995","unstructured":"Szijan, I., Lohmann, D.R., Parma, D.L., Brandt, B. & Horsthemke, B. Identification of RB1 germline mutations in Argentinian families with sporadic bilateral retinoblastoma. J. Med. Genet. 32, 475\u2013479 (1995).","journal-title":"J. Med. Genet."},{"key":"BFnm3600_CR45","doi-asserted-by":"publisher","first-page":"a000919","DOI":"10.1101\/cshperspect.a000919","volume":"2","author":"AC Joerger","year":"2010","unstructured":"Joerger, A.C. & Fersht, A.R. The tumor suppressor p53: from structures to drug discovery. Cold Spring Harb. Perspect. Biol. 2, a000919 (2010).","journal-title":"Cold Spring Harb. Perspect. Biol."},{"key":"BFnm3600_CR46","doi-asserted-by":"publisher","first-page":"124","DOI":"10.1186\/1472-6750-11-124","volume":"11","author":"SY Alcoser","year":"2011","unstructured":"Alcoser, S.Y. et al. Real-time PCR-based assay to quantify the relative amount of human and mouse tissue present in tumor xenografts. BMC Biotechnol. 11, 124 (2011).","journal-title":"BMC Biotechnol."},{"key":"BFnm3600_CR47","doi-asserted-by":"publisher","first-page":"6159","DOI":"10.1093\/nar\/gkq421","volume":"38","author":"AR Thierry","year":"2010","unstructured":"Thierry, A.R. et al. Origin and quantification of circulating DNA in mice with human colorectal cancer xenografts. Nucleic Acids Res. 38, 6159\u20136175 (2010).","journal-title":"Nucleic Acids Res."},{"key":"BFnm3600_CR48","doi-asserted-by":"publisher","first-page":"1358","DOI":"10.1038\/bjc.2013.92","volume":"108","author":"DJ Peeters","year":"2013","unstructured":"Peeters, D.J. et al. Semiautomated isolation and molecular characterisation of single or highly purified tumour cells from CellSearch enriched blood samples using dielectrophoretic cell sorting. Br. J. Cancer 108, 1358\u20131367 (2013).","journal-title":"Br. J. Cancer"},{"key":"BFnm3600_CR49","doi-asserted-by":"publisher","first-page":"1297","DOI":"10.1101\/gr.107524.110","volume":"20","author":"A McKenna","year":"2010","unstructured":"McKenna, A. et al. The Genome Analysis Toolkit: a MapReduce framework for analyzing next-generation DNA sequencing data. Genome Res. 20, 1297\u20131303 (2010).","journal-title":"Genome Res."},{"key":"BFnm3600_CR50","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1038\/ng.806","volume":"43","author":"MA DePristo","year":"2011","unstructured":"DePristo, M.A. et al. A framework for variation discovery and genotyping using next-generation DNA sequencing data. Nat. Genet. 43, 491\u2013498 (2011).","journal-title":"Nat. Genet."},{"key":"BFnm3600_CR51","doi-asserted-by":"publisher","first-page":"e164","DOI":"10.1093\/nar\/gkq603","volume":"38","author":"K Wang","year":"2010","unstructured":"Wang, K., Li, M. & Hakonarson, H. ANNOVAR: functional annotation of genetic variants from high-throughput sequencing data. Nucleic Acids Res. 38, e164 (2010).","journal-title":"Nucleic Acids Res."},{"key":"BFnm3600_CR52","doi-asserted-by":"publisher","first-page":"268","DOI":"10.1093\/bioinformatics\/btq635","volume":"27","author":"V Boeva","year":"2011","unstructured":"Boeva, V. et al. Control-free calling of copy number alterations in deep-sequencing data using GC-content normalization. Bioinformatics 27, 268\u2013269 (2011).","journal-title":"Bioinformatics"},{"key":"BFnm3600_CR53","doi-asserted-by":"publisher","first-page":"1639","DOI":"10.1101\/gr.092759.109","volume":"19","author":"M Krzywinski","year":"2009","unstructured":"Krzywinski, M. et al. Circos: an information aesthetic for comparative genomics. Genome Res. 19, 1639\u20131645 (2009).","journal-title":"Genome Res."},{"key":"BFnm3600_CR54","doi-asserted-by":"publisher","first-page":"D780","DOI":"10.1093\/nar\/gkm779","volume":"36","author":"T Yates","year":"2008","unstructured":"Yates, T., Okoniewski, M.J. & Miller, C.J. X:Map: annotation and visualization of genome structure for Affymetrix exon array analysis. Nucleic Acids Res. 36, D780\u2013D786 (2008).","journal-title":"Nucleic Acids Res."}],"container-title":["Nature Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/nm.3600.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/nm.3600","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/nm.3600.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,19]],"date-time":"2022-04-19T08:27:52Z","timestamp":1650356872000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nm.3600"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,6,1]]},"references-count":54,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["BFnm3600"],"URL":"https:\/\/doi.org\/10.1038\/nm.3600","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.718426567.793496778","asserted-by":"object"}]},"ISSN":["1078-8956","1546-170X"],"issn-type":[{"value":"1078-8956","type":"print"},{"value":"1546-170X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,6,1]]},"assertion":[{"value":"17 March 2014","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 May 2014","order":2,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"1 June 2014","order":3,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare no competing financial interests.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Competing interests"}}]}}