{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T08:24:37Z","timestamp":1792571077630,"version":"4.5.0"},"reference-count":64,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2006,8,1]],"date-time":"2006-08-01T00:00:00Z","timestamp":1154390400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nat Protoc"],"published-print":{"date-parts":[[2006,8]]},"DOI":"10.1038\/nprot.2006.228","type":"journal-article","created":{"date-parts":[[2006,11,2]],"date-time":"2006-11-02T19:59:29Z","timestamp":1162497569000},"page":"1439-1445","source":"Crossref","is-referenced-by-count":159,"title":["Preparation of organotypic hippocampal slice cultures: interface method"],"prefix":"10.1038","volume":"1","author":[{"given":"Anna","family":"De Simoni","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lily","family":"MY Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2006,11,2]]},"reference":[{"key":"BFnprot2006228_CR1","doi-asserted-by":"publisher","first-page":"484","DOI":"10.1016\/0166-2236(88)90007-0","volume":"11","author":"BH Gahwiler","year":"1988","unstructured":"Gahwiler, B.H. Organotypic cultures of neural tissue. Trends. Neurosci. 11, 484\u2013489 (1988).","journal-title":"Trends. Neurosci."},{"key":"BFnprot2006228_CR2","doi-asserted-by":"publisher","first-page":"343","DOI":"10.1016\/S0361-9230(99)00097-0","volume":"50","author":"BH Gahwiler","year":"1999","unstructured":"Gahwiler, B.H. Nerve cells in culture: the extraordinary discovery by Ross Granville Harrison. Brain. Res. Bull. 50, 343\u2013344 (1999).","journal-title":"Brain. Res. Bull."},{"key":"BFnprot2006228_CR3","doi-asserted-by":"publisher","first-page":"140","DOI":"10.3181\/00379727-4-98","volume":"4","author":"RG Harrison","year":"1907","unstructured":"Harrison, R.G. Observations on the living developing nerve fiber. Proc. Soc. Exp. Biol. Med. 4, 140\u2013143 (1907).","journal-title":"Proc. Soc. Exp. Biol. Med."},{"key":"BFnprot2006228_CR4","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1002\/jez.1401420103","volume":"142","author":"RG Harrison","year":"1959","unstructured":"Harrison, R.G. The outgrowth of the nerve fiber as a mode of protoplasmic movement. J. Exp. Zoo. 142, 5\u201373 (1959).","journal-title":"J. Exp. Zoo."},{"key":"BFnprot2006228_CR5","doi-asserted-by":"publisher","first-page":"201","DOI":"10.1002\/jez.a.20032","volume":"301","author":"H Keshishian","year":"2004","unstructured":"Keshishian, H. Ross Harrison's \u201cThe outgrowth of the nerve fiber as a mode of protoplasmic movement\u201d. J. Exp. Zool A Comp. Exp. Biol. 301, 201\u2013203 (2004).","journal-title":"J. Exp. Zool A Comp. Exp. Biol."},{"key":"BFnprot2006228_CR6","doi-asserted-by":"publisher","first-page":"329","DOI":"10.1016\/0165-0270(81)90003-0","volume":"4","author":"BH Gahwiler","year":"1981","unstructured":"Gahwiler, B.H. Organotypic monolayer cultures of nervous tissue. J. Neurosci. Methods 4, 329\u2013342 (1981).","journal-title":"J. Neurosci. Methods"},{"key":"BFnprot2006228_CR7","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1002\/jez.1401060104","volume":"106","author":"MJ Hogue","year":"2006","unstructured":"Hogue, M.J. Human fetal brain cells in tissue cultures: their identification and motility. J. Exp. Zool. 106, 85\u2013107 (2006).","journal-title":"J. Exp. Zool."},{"key":"BFnprot2006228_CR8","doi-asserted-by":"publisher","first-page":"173","DOI":"10.1016\/0165-0270(91)90128-M","volume":"37","author":"L Stoppini","year":"1991","unstructured":"Stoppini, L., Buchs, P.A. & Muller, D. A simple method for organotypic cultures of nervous tissue. J. Neurosci. Methods 37, 173\u2013182 (1991).","journal-title":"J. Neurosci. Methods"},{"key":"BFnprot2006228_CR9","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1016\/S0166-2236(97)01122-3","volume":"20","author":"BH Gahwiler","year":"1997","unstructured":"Gahwiler, B.H., Capogna, M., Debanne, D., McKinney, R.A. & Thompson, S.M. Organotypic slice cultures: a technique has come of age. Trends Neurosci. 20, 471\u2013477 (1997).","journal-title":"Trends Neurosci."},{"key":"BFnprot2006228_CR10","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1113\/jphysiol.2003.039099","volume":"550","author":"A De Simoni","year":"2003","unstructured":"De Simoni, A., Griesinger, C.B. & Edwards, F.A. Development of rat CA1 neurones in acute versus organotypic slices: role of experience in synaptic morphology and activity. J. Physiol. 550, 135\u2013147 (2003).","journal-title":"J. Physiol."},{"key":"BFnprot2006228_CR11","doi-asserted-by":"publisher","first-page":"8040","DOI":"10.1073\/pnas.93.15.8040","volume":"93","author":"PA Buchs","year":"1996","unstructured":"Buchs, P.A. & Muller, D. Induction of long-term potentiation is associated with major ultrastructural changes of activated synapses. Proc. Natl. Acad. Sci. USA 93, 8040\u20138045 (1996).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnprot2006228_CR12","doi-asserted-by":"publisher","first-page":"11225","DOI":"10.1073\/pnas.93.20.11225","volume":"93","author":"D Debanne","year":"1996","unstructured":"Debanne, D., Gahwiler, B.H. & Thompson, S.M. Cooperative interactions in the induction of long-term potentiation and depression of synaptic excitation between hippocampal CA3\u2013CA1 cell pairs in vitro. Proc. Natl. Acad. Sci. USA 93, 11225\u201311230 (1996).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnprot2006228_CR13","doi-asserted-by":"publisher","first-page":"1923","DOI":"10.1126\/science.283.5409.1923","volume":"283","author":"M Maletic-Savatic","year":"1999","unstructured":"Maletic-Savatic, M., Malinow, R. & Svoboda, K. Rapid dendritic morphogenesis in CA1 hippocampal dendrites induced by synaptic activity. Science 283, 1923\u20131927 (1999).","journal-title":"Science"},{"key":"BFnprot2006228_CR14","doi-asserted-by":"publisher","first-page":"1614","DOI":"10.1152\/jn.1997.77.3.1614","volume":"77","author":"C Collin","year":"1997","unstructured":"Collin, C., Miyaguchi, K. & Segal, M. Dendritic spine density and LTP induction in cultured hippocampal slices. J. Neurophysiol 77, 1614\u20131623 (1997).","journal-title":"J. Neurophysiol"},{"key":"BFnprot2006228_CR15","doi-asserted-by":"publisher","first-page":"6131","DOI":"10.1073\/pnas.0501145102","volume":"102","author":"Z Li","year":"2005","unstructured":"Li, Z. et al. Synaptic vesicle recycling studied in transgenic mice expressing synaptopHluorin. Proc. Natl. Acad. Sci. USA 102, 6131\u20136136 (2005).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnprot2006228_CR16","doi-asserted-by":"publisher","first-page":"66","DOI":"10.1038\/19978","volume":"399","author":"F Engert","year":"1999","unstructured":"Engert, F. & Bonhoeffer, T. Dendritic spine changes associated with hippocampal long-term synaptic plasticity. Nature 399, 66\u201370 (1999).","journal-title":"Nature"},{"key":"BFnprot2006228_CR17","doi-asserted-by":"publisher","first-page":"289","DOI":"10.1016\/j.neuron.2005.08.034","volume":"48","author":"A Barria","year":"2005","unstructured":"Barria, A. & Malinow, R. NMDA receptor subunit composition controls synaptic plasticity by regulating binding to CaMKII. Neuron 48, 289\u2013301 (2005).","journal-title":"Neuron"},{"key":"BFnprot2006228_CR18","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1016\/S0165-0270(03)00228-0","volume":"130","author":"JK Leutgeb","year":"2003","unstructured":"Leutgeb, J.K., Frey, J.U. & Behnisch, T. LTP in cultured hippocampal-entorhinal cortex slices from young adult (P25\u201330) rats. J. Neurosci. Methods 130, 19\u201332 (2003).","journal-title":"J. Neurosci. Methods"},{"key":"BFnprot2006228_CR19","doi-asserted-by":"publisher","first-page":"509","DOI":"10.1006\/mthe.2002.0588","volume":"5","author":"CP Glover","year":"2002","unstructured":"Glover, C.P., Bienemann, A.S., Heywood, D.J., Cosgrave, A.S. & Uney, J.B. Adenoviral-mediated, high-level, cell-specific transgene expression: a SYN1-WPRE cassette mediates increased transgene expression with no loss of neuron specificity. Mol. Ther. 5, 509\u2013516 (2002).","journal-title":"Mol. Ther."},{"key":"BFnprot2006228_CR20","doi-asserted-by":"publisher","first-page":"195","DOI":"10.1016\/S0361-9230(99)00217-8","volume":"51","author":"K Miyaguchi","year":"2000","unstructured":"Miyaguchi, K., Maeda, Y., Collin, C. & Sihag, R.K. Gene transfer into hippocampal slice cultures with an adenovirus vector driven by cytomegalovirus promoter: stable co-expression of green fluorescent protein and lacZ genes. Brain Res. Bull. 51, 195\u2013202 (2000).","journal-title":"Brain Res. Bull."},{"key":"BFnprot2006228_CR21","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1038\/nn1046","volume":"6","author":"V De Paola","year":"2003","unstructured":"De Paola, V., Arber, S. & Caroni, P. AMPA receptors regulate dynamic equilibrium of presynaptic terminals in mature hippocampal networks. Nat. Neurosci. 6, 491\u2013500 (2003).","journal-title":"Nat. Neurosci."},{"key":"BFnprot2006228_CR22","unstructured":"Stricker, C. in Neuroscience Methods (ed. Martin, R.) (Harwood Academic Publishers, Amsterdam, 1997)."},{"key":"BFnprot2006228_CR23","doi-asserted-by":"publisher","first-page":"759","DOI":"10.1016\/j.neuron.2004.11.016","volume":"44","author":"UV Nagerl","year":"2004","unstructured":"Nagerl, U.V., Eberhorn, N., Cambridge, S.B. & Bonhoeffer, T. Bidirectional activity-dependent morphological plasticity in hippocampal neurons. Neuron 44, 759\u2013767 (2004).","journal-title":"Neuron"},{"key":"BFnprot2006228_CR24","doi-asserted-by":"publisher","first-page":"749","DOI":"10.1016\/j.neuron.2006.04.026","volume":"50","author":"I Galimberti","year":"2006","unstructured":"Galimberti, I. et al. Long-term rearrangements of hippocampal mossy fiber terminal connectivity in the adult regulated by experience. Neuron 50, 749\u2013763 (2006).","journal-title":"Neuron"},{"key":"BFnprot2006228_CR25","doi-asserted-by":"publisher","first-page":"241","DOI":"10.1016\/j.mcn.2004.01.003","volume":"26","author":"O Raineteau","year":"2004","unstructured":"Raineteau, O., Rietschin, L., Gradwohl, G., Guillemot, F. & Gahwiler, B.H. Neurogenesis in hippocampal slice cultures. Mol. Cell. Neurosci. 26, 241\u2013250 (2004).","journal-title":"Mol. Cell. Neurosci."},{"key":"BFnprot2006228_CR26","doi-asserted-by":"publisher","first-page":"185","DOI":"10.1016\/S0165-3806(02)00590-4","volume":"140","author":"I Nikonenko","year":"2003","unstructured":"Nikonenko, I. et al. Integrins are involved in synaptogenesis, cell spreading, and adhesion in the postnatal brain. Brain Res. Dev. Brain Res. 140, 185\u2013194 (2003).","journal-title":"Brain Res. Dev. Brain Res."},{"key":"BFnprot2006228_CR27","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/0306-4522(95)00174-H","volume":"68","author":"R Linke","year":"1995","unstructured":"Linke, R., Heimrich, B. & Frotscher, M. Axonal regeneration of identified septohippocampal projection neurons in vitro. Neuroscience 68, 1\u20134 (1995).","journal-title":"Neuroscience"},{"key":"BFnprot2006228_CR28","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/S0014-5793(01)02707-7","volume":"504","author":"K Lundstrom","year":"2001","unstructured":"Lundstrom, K. et al. Semliki Forest virus vectors: efficient vehicles for in vitro and in vivo gene delivery. FEBS Lett. 504, 99\u2013103 (2001).","journal-title":"FEBS Lett."},{"key":"BFnprot2006228_CR29","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1016\/S1525-0016(02)00056-4","volume":"7","author":"K Lundstrom","year":"2003","unstructured":"Lundstrom, K., Abenavoli, A., Malgaroli, A. & Ehrengruber, M.U. Novel Semliki Forest virus vectors with reduced cytotoxicity and temperature sensitivity for long-term enhancement of transgene expression. Mol. Ther. 7, 202\u2013209 (2003).","journal-title":"Mol. Ther."},{"key":"BFnprot2006228_CR30","doi-asserted-by":"publisher","first-page":"101","DOI":"10.1111\/j.1460-9568.2004.03461.x","volume":"20","author":"W Kakegawa","year":"2004","unstructured":"Kakegawa, W., Tsuzuki, K., Yoshida, Y., Kameyama, K. & Ozawa, S. Input- and subunit-specific AMPA receptor trafficking underlying long-term potentiation at hippocampal CA3 synapses. Eur. J. Neurosci. 20, 101\u2013110 (2004).","journal-title":"Eur. J. Neurosci."},{"key":"BFnprot2006228_CR31","doi-asserted-by":"publisher","first-page":"7041","DOI":"10.1073\/pnas.96.12.7041","volume":"96","author":"MU Ehrengruber","year":"1999","unstructured":"Ehrengruber, M.U. et al. Recombinant Semliki Forest virus and Sindbis virus efficiently infect neurons in hippocampal slice cultures. Proc. Natl. Acad. Sci. USA 96, 7041\u20137046 (1999).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnprot2006228_CR32","doi-asserted-by":"publisher","first-page":"9085","DOI":"10.1523\/JNEUROSCI.17-23-09085.1997","volume":"17","author":"JL Perez Velazquez","year":"1997","unstructured":"Perez Velazquez, J.L., Frantseva, M.V. & Carlen, P.L. In vitro ischemia promotes glutamate-mediated free radical generation and intracellular calcium accumulation in hippocampal pyramidal neurons. J. Neurosci. 17, 9085\u20139094 (1997).","journal-title":"J. Neurosci."},{"key":"BFnprot2006228_CR33","doi-asserted-by":"publisher","first-page":"7702","DOI":"10.1523\/JNEUROSCI.15-11-07702.1995","volume":"15","author":"DW Newell","year":"1995","unstructured":"Newell, D.W., Barth, A., Papermaster, V. & Malouf, A.T. Glutamate and non-glutamate receptor mediated toxicity caused by oxygen and glucose deprivation in organotypic hippocampal cultures. J. Neurosci. 15, 7702\u20137711 (1995).","journal-title":"J. Neurosci."},{"key":"BFnprot2006228_CR34","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/S0165-5728(00)00402-1","volume":"113","author":"H Schmidt","year":"2001","unstructured":"Schmidt, H. et al. Organotypic hippocampal cultures. A model of brain tissue damage in Streptococcus pneumoniae meningitis. J. Neuroimmunol. 113, 30\u201339 (2001).","journal-title":"J. Neuroimmunol."},{"key":"BFnprot2006228_CR35","doi-asserted-by":"publisher","first-page":"610","DOI":"10.1093\/jnen\/63.6.610","volume":"63","author":"C Gianinazzi","year":"2004","unstructured":"Gianinazzi, C. et al. Apoptosis of hippocampal neurons in organotypic slice culture models: direct effect of bacteria revisited. J. Neuropathol. Exp. Neurol. 63, 610\u2013617 (2004).","journal-title":"J. Neuropathol. Exp. Neurol."},{"key":"BFnprot2006228_CR36","doi-asserted-by":"publisher","first-page":"207","DOI":"10.1002\/cne.903250206","volume":"325","author":"T Naumann","year":"1992","unstructured":"Naumann, T., Linke, R. & Frotscher, M. Fine structure of rat septohippocampal neurons: I. Identification of septohippocampal projection neurons by retrograde tracing combined with electron microscopic immunocytochemistry and intracellular staining. J. Comp. Neurol. 325, 207\u2013218 (1992).","journal-title":"J. Comp. Neurol."},{"key":"BFnprot2006228_CR37","doi-asserted-by":"publisher","first-page":"209","DOI":"10.1002\/cne.903620205","volume":"362","author":"BW Coltman","year":"1995","unstructured":"Coltman, B.W., Earley, E.M., Shahar, A., Dudek, F.E. & Ide, C.F. Factors influencing mossy fiber collateral sprouting in organotypic slice cultures of neonatal mouse hippocampus. J. Comp. Neurol. 362, 209\u2013222 (1995).","journal-title":"J. Comp. Neurol."},{"key":"BFnprot2006228_CR38","doi-asserted-by":"publisher","first-page":"7953","DOI":"10.1073\/pnas.1332667100","volume":"100","author":"AM Ghoumari","year":"2003","unstructured":"Ghoumari, A.M. et al. Mifepristone (RU486) protects Purkinje cells from cell death in organotypic slice cultures of postnatal rat and mouse cerebellum. Proc. Natl. Acad. Sci. USA 100, 7953\u20137958 (2003).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnprot2006228_CR39","doi-asserted-by":"publisher","first-page":"553","DOI":"10.1002\/(SICI)1097-4547(19991115)58:4<553::AID-JNR8>3.0.CO;2-8","volume":"58","author":"H Becq","year":"1999","unstructured":"Becq, H., Bosler, O., Geffard, M., Enjalbert, A. & Herman, J.P. Anatomical and functional reconstruction of the nigrostriatal system in vitro: selective innervation of the striatum by dopaminergic neurons. J. Neurosci. Res. 58, 553\u2013566 (1999).","journal-title":"J. Neurosci. Res."},{"key":"BFnprot2006228_CR40","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1089\/089771504322972121","volume":"21","author":"Y Oishi","year":"2004","unstructured":"Oishi, Y., Baratta, J., Robertson, R.T. & Steward, O. Assessment of factors regulating axon growth between the cortex and spinal cord in organotypic co-cultures: effects of age and neurotrophic factors. J. Neurotrauma 21, 339\u2013356 (2004).","journal-title":"J. Neurotrauma"},{"key":"BFnprot2006228_CR41","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/j.brainresrev.2004.07.016","volume":"46","author":"KJ Hilton","year":"2004","unstructured":"Hilton, K.J., Bateson, A.N. & King, A.E. A model of organotypic rat spinal slice culture and biolistic transfection to elucidate factors that drive the preprotachykinin-A promoter. Brain Res. Brain. Res. Rev. 46, 191\u2013203 (2004).","journal-title":"Brain Res. Brain. Res. Rev."},{"key":"BFnprot2006228_CR42","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1016\/S0306-4522(01)00472-9","volume":"109","author":"H Takuma","year":"2002","unstructured":"Takuma, H., Sakurai, M. & Kanazawa, I. In vitro formation of corticospinal synapses in an organotypic slice co-culture. Neuroscience 109, 359\u2013370 (2002).","journal-title":"Neuroscience"},{"key":"BFnprot2006228_CR43","doi-asserted-by":"publisher","first-page":"841","DOI":"10.1016\/S0736-5748(96)00056-1","volume":"14","author":"Q Gong","year":"1996","unstructured":"Gong, Q., Liu, W.L., Srodon, M., Foster, T.D. & Shipley, M.T. Olfactory epithelial organotypic slice cultures: a useful tool for investigating olfactory neural development. Int. J. Dev. Neurosci. 14, 841\u2013852 (1996).","journal-title":"Int. J. Dev. Neurosci."},{"key":"BFnprot2006228_CR44","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1006\/exnr.1999.7032","volume":"156","author":"Z Molnar","year":"1999","unstructured":"Molnar, Z. & Blakemore, C. Development of signals influencing the growth and termination of thalamocortical axons in organotypic culture. Exp. Neurol. 156, 363\u2013393 (1999).","journal-title":"Exp. Neurol."},{"key":"BFnprot2006228_CR45","doi-asserted-by":"publisher","first-page":"645","DOI":"10.1038\/nature00779","volume":"417","author":"K Letinic","year":"2002","unstructured":"Letinic, K., Zoncu, R. & Rakic, P. Origin of GABAergic neurons in the human neocortex. Nature 417, 645\u2013649 (2002).","journal-title":"Nature"},{"key":"BFnprot2006228_CR46","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1016\/j.jneumeth.2005.01.017","volume":"146","author":"HW Dong","year":"2005","unstructured":"Dong, H.W. & Buonomano, D.V. A technique for repeated recordings in cortical organotypic slices. J. Neurosci. Methods 146, 69\u201375 (2005).","journal-title":"J. Neurosci. Methods"},{"key":"BFnprot2006228_CR47","doi-asserted-by":"publisher","first-page":"60","DOI":"10.1016\/S1385-299X(02)00180-0","volume":"10","author":"AV Krassioukov","year":"2002","unstructured":"Krassioukov, A.V. et al. An in vitro model of neurotrauma in organotypic spinal cord cultures from adult mice. Brain Res. Brain Res. Protoc. 10, 60\u201368 (2002).","journal-title":"Brain Res. Brain Res. Protoc."},{"key":"BFnprot2006228_CR48","doi-asserted-by":"publisher","first-page":"9282","DOI":"10.1523\/JNEUROSCI.2120-04.2004","volume":"24","author":"VJ Tom","year":"2004","unstructured":"Tom, V.J., Doller, C.M., Malouf, A.T. & Silver, J. Astrocyte-associated fibronectin is critical for axonal regeneration in adult white matter. J. Neurosci. 24, 9282\u20139290 (2004).","journal-title":"J. Neurosci."},{"key":"BFnprot2006228_CR49","doi-asserted-by":"publisher","first-page":"564","DOI":"10.1124\/mol.105.016683","volume":"69","author":"Z Mtchedlishvili","year":"2006","unstructured":"Mtchedlishvili, Z. & Kapur, J. High-affinity, slowly desensitizing GABAA receptors mediate tonic inhibition in hippocampal dentate granule cells. Mol. Pharmacol. 69, 564\u2013575 (2006).","journal-title":"Mol. Pharmacol."},{"key":"BFnprot2006228_CR50","doi-asserted-by":"publisher","first-page":"1263","DOI":"10.1016\/0896-6273(94)90412-X","volume":"13","author":"DC Lo","year":"1994","unstructured":"Lo, D.C., McAllister, A.K. & Katz, L.C. Neuronal transfection in brain slices using particle-mediated gene transfer. Neuron 13, 1263\u20131268 (1994).","journal-title":"Neuron"},{"key":"BFnprot2006228_CR51","doi-asserted-by":"publisher","first-page":"791","DOI":"10.1016\/0896-6273(95)90171-X","volume":"15","author":"AK McAllister","year":"1995","unstructured":"McAllister, A.K., Lo, D.C. & Katz, L.C. Neurotrophins regulate dendritic growth in developing visual cortex. Neuron 15, 791\u2013803 (1995).","journal-title":"Neuron"},{"key":"BFnprot2006228_CR52","first-page":"197","volume":"245","author":"AK McAllister","year":"2004","unstructured":"McAllister, A.K. Biolistic transfection of cultured organotypic brain slices. Methods Mol. Biol. 245, 197\u2013206 (2004).","journal-title":"Methods Mol. Biol."},{"key":"BFnprot2006228_CR53","doi-asserted-by":"publisher","first-page":"57","DOI":"10.1016\/S0165-0270(01)00457-5","volume":"112","author":"JA O'Brien","year":"2001","unstructured":"O'Brien, J.A., Holt, M., Whiteside, G., Lummis, S.C. & Hastings, M.H. Modifications to the hand-held gene gun: improvements for in vitro biolistic transfection of organotypic neuronal tissue. J. Neurosci. Methods 112, 57\u201364 (2001).","journal-title":"J. Neurosci. Methods"},{"key":"BFnprot2006228_CR54","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/S0165-0270(03)00069-4","volume":"126","author":"J Rathenberg","year":"2003","unstructured":"Rathenberg, J., Nevian, T. & Witzemann, V. High-efficiency transfection of individual neurons using modified electrophysiology techniques. J. Neurosci. Methods 126, 91\u201398 (2003).","journal-title":"J. Neurosci. Methods"},{"key":"BFnprot2006228_CR55","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1016\/0165-3806(82)90071-2","volume":"255","author":"RL Beach","year":"1982","unstructured":"Beach, R.L., Bathgate, S.L. & Cotman, C.W. Identification of cell types in rat hippocampal slices maintained in organotypic cultures. Brain Res. 255, 3\u201320 (1982).","journal-title":"Brain Res."},{"key":"BFnprot2006228_CR56","doi-asserted-by":"publisher","first-page":"41","DOI":"10.1016\/j.jneumeth.2004.05.013","volume":"141","author":"AM Benediktsson","year":"2005","unstructured":"Benediktsson, A.M., Schachtele, S.J., Green, S.H. & Dailey, M.E. Ballistic labeling and dynamic imaging of astrocytes in organotypic hippocampal slice cultures. J. Neurosci. Methods 141, 41\u201353 (2005).","journal-title":"J. Neurosci. Methods"},{"key":"BFnprot2006228_CR57","doi-asserted-by":"publisher","first-page":"4147","DOI":"10.1073\/pnas.93.9.4147","volume":"93","author":"JA London","year":"1996","unstructured":"London, J.A., Biegel, D. & Pachter, J.S. Neurocytopathic effects of beta-amyloid-stimulated monocytes: a potential mechanism for central nervous system damage in Alzheimer disease. Proc. Natl. Acad. Sci. USA 93, 4147\u20134152 (1996).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnprot2006228_CR58","doi-asserted-by":"publisher","first-page":"435","DOI":"10.2174\/1568007054546108","volume":"4","author":"J Noraberg","year":"2005","unstructured":"Noraberg, J. et al. Organotypic hippocampal slice cultures for studies of brain damage, neuroprotection and neurorepair. Curr. Drug Targets CNS Neurol. Disord. 4, 435\u2013452 (2005).","journal-title":"Curr. Drug Targets CNS Neurol. Disord."},{"key":"BFnprot2006228_CR59","doi-asserted-by":"publisher","first-page":"278","DOI":"10.1016\/S1385-299X(98)00050-6","volume":"3","author":"J Noraberg","year":"1999","unstructured":"Noraberg, J., Kristensen, B.W. & Zimmer, J. Markers for neuronal degeneration in organotypic slice cultures. Brain Res. Brain Res. Protoc. 3, 278\u2013290 (1999).","journal-title":"Brain Res. Brain Res. Protoc."},{"key":"BFnprot2006228_CR60","doi-asserted-by":"publisher","first-page":"1109","DOI":"10.1016\/S0028-3908(02)00052-7","volume":"42","author":"JA Kim","year":"2002","unstructured":"Kim, J.A. et al. Cytoskeleton disruption causes apoptotic degeneration of dentate granule cells in hippocampal slice cultures. Neuropharmacology 42, 1109\u20131118 (2002).","journal-title":"Neuropharmacology"},{"key":"BFnprot2006228_CR61","doi-asserted-by":"publisher","first-page":"921","DOI":"10.1038\/sj.cdd.4400884","volume":"8","author":"F Moroni","year":"2001","unstructured":"Moroni, F. et al. Poly(ADP-ribose) polymerase inhibitors attenuate necrotic but not apoptotic neuronal death in experimental models of cerebral ischemia. Cell Death Differ. 8, 921\u2013932 (2001).","journal-title":"Cell Death Differ."},{"key":"BFnprot2006228_CR62","doi-asserted-by":"publisher","first-page":"197","DOI":"10.1016\/S0079-6123(02)35019-2","volume":"135","author":"U Heinemann","year":"2002","unstructured":"Heinemann, U. et al. Cell death and metabolic activity during epileptiform discharges and status epilepticus in the hippocampus. Prog. Brain Res. 135, 197\u2013210 (2002).","journal-title":"Prog. Brain Res."},{"issue":"Suppl. 5","key":"BFnprot2006228_CR63","doi-asserted-by":"publisher","first-page":"168","DOI":"10.1046\/j.1528-1157.43.s.5.15.x","volume":"43","author":"U Heinemann","year":"2002","unstructured":"Heinemann, U. et al. Coupling of electrical and metabolic activity during epileptiform discharges. Epilepsia 43 (Suppl. 5): 168\u2013173 (2002).","journal-title":"Epilepsia"},{"key":"BFnprot2006228_CR64","doi-asserted-by":"publisher","first-page":"2909","DOI":"10.1152\/jn.00149.2002","volume":"88","author":"R Kovacs","year":"2002","unstructured":"Kovacs, R. et al. Free radical-mediated cell damage after experimental status epilepticus in hippocampal slice cultures. J. Neurophysiol 88, 2909\u20132918 (2002).","journal-title":"J. Neurophysiol"}],"container-title":["Nature Protocols"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nprot.2006.228.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nprot.2006.228","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nprot.2006.228.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T00:01:29Z","timestamp":1684454489000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nprot.2006.228"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,8]]},"references-count":64,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2006,8]]}},"alternative-id":["BFnprot2006228"],"URL":"https:\/\/doi.org\/10.1038\/nprot.2006.228","relation":{},"ISSN":["1754-2189","1750-2799"],"issn-type":[{"value":"1754-2189","type":"print"},{"value":"1750-2799","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,8]]}}}