{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T21:07:04Z","timestamp":1789765624687,"version":"4.0.1"},"reference-count":9,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2006,12,1]],"date-time":"2006-12-01T00:00:00Z","timestamp":1164931200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nat Protoc"],"published-print":{"date-parts":[[2006,12]]},"DOI":"10.1038\/nprot.2006.434","type":"journal-article","created":{"date-parts":[[2007,1,26]],"date-time":"2007-01-26T20:11:34Z","timestamp":1169842294000},"page":"2933-2939","source":"Crossref","is-referenced-by-count":45,"title":["Assays for in vitro monitoring of human airway smooth muscle (ASM) and human pulmonary arterial vascular smooth muscle (VSM) cell migration"],"prefix":"10.1038","volume":"1","author":[{"given":"Elena A","family":"Goncharova","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry A","family":"Goncharov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera P","family":"Krymskaya","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2007,1,25]]},"reference":[{"key":"BFnprot2006434_CR1","doi-asserted-by":"publisher","first-page":"453","DOI":"10.1084\/jem.115.3.453","volume":"115","author":"S Boyden","year":"1962","unstructured":"Boyden, S. Chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leukocytes. J. Exp. Med. 115, 453\u2013466 (1962).","journal-title":"J. Exp. Med."},{"key":"BFnprot2006434_CR2","doi-asserted-by":"publisher","first-page":"606","DOI":"10.1083\/jcb.75.2.606","volume":"75","author":"SH Zigmond","year":"1977","unstructured":"Zigmond, S.H. Ability of polymorphonuclear leukocytes to orient in gradients of chemotactic factors. J.Cell Biol. 75, 606\u2013616 (1977).","journal-title":"J.Cell Biol."},{"key":"BFnprot2006434_CR3","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1165\/rcmb.2002-0254OC","volume":"29","author":"EA Goncharova","year":"2003","unstructured":"Goncharova, E.A. et al. Cyclic AMP-mobilizing agents and glucocorticoids modulate human smooth muscle cell migration. Am. J. Respir. Cell Mol. Biol. 29, 19\u201327 (2003).","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"BFnprot2006434_CR4","doi-asserted-by":"publisher","first-page":"115","DOI":"10.1515\/BC.2002.012","volume":"383","author":"EA Goncharova","year":"2002","unstructured":"Goncharova, E.A. et al. Activation of p38 MAP-kinase and caldesmon phosphorylation are essential for urokinase-induced human smooth muscle cell migration. Biol. Chem. 383, 115\u2013126 (2002).","journal-title":"Biol. Chem."},{"key":"BFnprot2006434_CR5","first-page":"L354","volume":"283","author":"EA Goncharova","year":"2002","unstructured":"Goncharova, E.A. et al. PI3K is required for proliferation and migration of human pulmonary vascular smooth muscle cells. Am. J. Physiol. 283, L354\u2013L363 (2002).","journal-title":"Am. J. Physiol."},{"key":"BFnprot2006434_CR6","doi-asserted-by":"publisher","first-page":"1171","DOI":"10.1083\/jcb.200405130","volume":"167","author":"E Goncharova","year":"2004","unstructured":"Goncharova, E., Goncharov, D., Noonan, D. & Krymskaya, V.P. TSC2 modulates actin cytoskeleton and focal adhesion through TSC1-binding domain and the Rac1 GTPase. J. Cell Biol. 167, 1171\u20131182 (2004).","journal-title":"J. Cell Biol."},{"key":"BFnprot2006434_CR7","doi-asserted-by":"publisher","first-page":"473","DOI":"10.1165\/rcmb.2005-0374OC","volume":"34","author":"EA Goncharova","year":"2006","unstructured":"Goncharova, E.A., Goncharov, D.A., Lim, P.N., Noonan, D. & Krymskaya, V.P. Modulation of cell migration and invasiveness by tumor suppressor TSC2 in lymphangioleiomyomatosis. Am. J. Respir. Cell Mol. Biol. 34, 473\u2013480 (2006).","journal-title":"Am. J. Respir. Cell Mol. Biol."},{"key":"BFnprot2006434_CR8","doi-asserted-by":"publisher","first-page":"729","DOI":"10.1016\/S0898-6568(03)00040-8","volume":"15","author":"VP Krymskaya","year":"2003","unstructured":"Krymskaya, V.P. Tumour suppressors hamartin and tuberin: intracellular signalling. Cell. Signal. 15, 729\u2013739 (2003).","journal-title":"Cell. Signal."},{"key":"BFnprot2006434_CR9","doi-asserted-by":"publisher","first-page":"19","DOI":"10.1038\/ng1494","volume":"37","author":"K Inoki","year":"2005","unstructured":"Inoki, K., Corradetti, M.N. & Guan, K.-L. Dysregulation of the TSC-mTOR pathway in human disease. Nat. Genet. 37, 19\u201324 (2005).","journal-title":"Nat. Genet."}],"container-title":["Nature Protocols"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nprot.2006.434.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nprot.2006.434","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nprot.2006.434.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T00:03:33Z","timestamp":1684454613000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nprot.2006.434"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,12]]},"references-count":9,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2006,12]]}},"alternative-id":["BFnprot2006434"],"URL":"https:\/\/doi.org\/10.1038\/nprot.2006.434","relation":{},"ISSN":["1754-2189","1750-2799"],"issn-type":[{"value":"1754-2189","type":"print"},{"value":"1750-2799","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,12]]}}}