{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T21:38:01Z","timestamp":1787002681868,"version":"build-2736575974"},"reference-count":14,"publisher":"Springer Science and Business Media LLC","issue":"4","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nat Protoc"],"published-print":{"date-parts":[[2007,4]]},"DOI":"10.1038\/nprot.2007.144","type":"journal-article","created":{"date-parts":[[2007,4,19]],"date-time":"2007-04-19T12:15:20Z","timestamp":1176984920000},"page":"972-978","source":"Crossref","is-referenced-by-count":35,"title":["An improved synthesis of NHS-MAG3 for conjugation and radiolabeling of biomolecules with 99mTc at room temperature"],"prefix":"10.1038","volume":"2","author":[{"given":"Yi","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinrong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald J","family":"Hnatowich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2007,4,19]]},"reference":[{"key":"BFnprot2007144_CR1","first-page":"56","volume":"39","author":"DJ Hnatowich","year":"1998","unstructured":"Hnatowich, D.J. et al. Labeling peptides with technetium-99m using a bifunctional chelator of a N-hydroxysuccinimide ester of mercaptoacetyltriglycine. J. Nucl. Med. 39, 56\u201364 (1998).","journal-title":"J. Nucl. Med."},{"key":"BFnprot2007144_CR2","doi-asserted-by":"publisher","first-page":"4958","DOI":"10.1158\/1078-0432.CCR-06-0844","volume":"12","author":"G Liu","year":"2006","unstructured":"Liu, G. et al. Successful radiotherapy of tumor in pretargeted mice by 188Re-radiolabeled phosphorodiamidate morpholino oligomer, a synthetic DNA analogue. Clin. Cancer Res. 12, 4958\u20134964 (2006).","journal-title":"Clin. Cancer Res."},{"key":"BFnprot2007144_CR3","doi-asserted-by":"publisher","first-page":"917","DOI":"10.1016\/S0969-8051(96)00131-X","volume":"23","author":"K Lei","year":"1996","unstructured":"Lei, K. et al. Technetium-99m antibodies labeled with MAG3 and SHNH: an in vitro and animal in vivo comparison. Nucl. Med. Biol. 23, 917\u2013922 (1996).","journal-title":"Nucl. Med. Biol."},{"key":"BFnprot2007144_CR4","doi-asserted-by":"publisher","first-page":"1700","DOI":"10.1007\/s002590000343","volume":"27","author":"Y Zhang","year":"2000","unstructured":"Zhang, Y., Liu, N., Zhu, Z., Rusckowski, M. & Hnatowich, D.J. Influence of different chelators (HYNIC, MAG3 and DTPA) on tumor cell accumulation and mouse biodistribution of technetium-99m labeled to antisense DNA. Eur. J. Nucl. Med. 27, 1700\u20131707 (2000).","journal-title":"Eur. J. Nucl. Med."},{"key":"BFnprot2007144_CR5","doi-asserted-by":"publisher","first-page":"135","DOI":"10.1016\/j.nucmedbio.2005.09.002","volume":"33","author":"JL Vanderheyden","year":"2006","unstructured":"Vanderheyden, J.L. et al. Evaluation of 99mTc-MAG3-annexin V: influence of the chelate on in vitro and in vivo properties in mice. Nucl. Med. Biol. 33, 135\u2013144 (2006).","journal-title":"Nucl. Med. Biol."},{"key":"BFnprot2007144_CR6","doi-asserted-by":"publisher","first-page":"651","DOI":"10.1016\/S0969-8051(98)00040-7","volume":"25","author":"OK Hjelstuen","year":"1998","unstructured":"Hjelstuen, O.K., Tonnesen, H.H., Bremer, P.O. & Verbruggen, A.M. 3\u2032-99mTc-labeling and biodistribution of a CAPL antisense oligodeoxynucleotide. Nucl. Med. Biol. 25, 651\u2013657 (1998).","journal-title":"Nucl. Med. Biol."},{"key":"BFnprot2007144_CR7","first-page":"111","volume":"27","author":"AR Fritzberg","year":"1986","unstructured":"Fritzberg, A.R., Kasina, S., Eshima, D. & Johnson, D.L. Synthesis and biological evaluation of technetium-99m MAG3 as a hippuran replacement. J. Nucl. Med. 27, 111\u2013116 (1986).","journal-title":"J. Nucl. Med."},{"key":"BFnprot2007144_CR8","doi-asserted-by":"publisher","first-page":"611","DOI":"10.1016\/S0969-8051(98)00022-5","volume":"25","author":"FB van Gog","year":"1998","unstructured":"van Gog, F.B., Visser, G.W., Gowrising, R.W., Snow, G.B. & van Dongen, G.A. Synthesis and evaluation of 99mTc\/99Tc-MAG3-Biotin conjugates for antibody pretargeting strategies. Nucl. Med. Biol. 25, 611\u2013619 (1998).","journal-title":"Nucl. Med. Biol."},{"key":"BFnprot2007144_CR9","doi-asserted-by":"publisher","first-page":"49","DOI":"10.1089\/cbr.2006.21.49","volume":"21","author":"SM Okarvi","year":"2006","unstructured":"Okarvi, S.M. & Jammaz, I.A. Preparation and in vitro and in vivo evaluation of technetium-99m-labeled folate and methotrexate conjugates as tumor imaging agents. Cancer Biother. Radiopharm. 21, 49\u201360 (2006).","journal-title":"Cancer Biother. Radiopharm."},{"key":"BFnprot2007144_CR10","first-page":"1201","volume":"45","author":"M Rusckowski","year":"2004","unstructured":"Rusckowski, M., Gupta, S., Liu, G., Dou, S. & Hnatowich, D.J. Investigations of a Tc-99m-labeled bacteriophage as a potential infection-specific imaging agent. J. Nucl. Med. 45, 1201\u20131208 (2004).","journal-title":"J. Nucl. Med."},{"key":"BFnprot2007144_CR11","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1016\/0003-2697(83)90426-8","volume":"132","author":"RJS Duncan","year":"1983","unstructured":"Duncan, R.J.S., Weston, P.D. & Wrigglesworth, R. A new reagent which may be used to introduce sulfhydryl-groups into proteins, and its use in the preparation of conjugates for immunoassay. Anal. Biochem. 132, 68\u201373 (1983).","journal-title":"Anal. Biochem."},{"key":"BFnprot2007144_CR12","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1021\/bc980121c","volume":"10","author":"C Decristoforo","year":"1999","unstructured":"Decristoforo, C. & Mather, S.J. Preparation, 99mTc-labeling, and in vitro characterization of HYNIC and N3S modified RC-160 and [Tyr3]octreotide. Bioconjugate Chem. 10, 431\u2013438 (1999).","journal-title":"Bioconjugate Chem."},{"key":"BFnprot2007144_CR13","doi-asserted-by":"publisher","first-page":"1477","DOI":"10.1038\/nprot.2006.262","volume":"1","author":"Y Wang","year":"2006","unstructured":"Wang, Y., Liu, G. & Hnatowich, D.J. Methods for MAG3 conjugation and 99mTc radiolabeling of biomolecules. Nat. Protocols 1, 1477\u20131480 (2006).","journal-title":"Nat. Protocols"},{"key":"BFnprot2007144_CR14","doi-asserted-by":"publisher","first-page":"425","DOI":"10.1016\/S0969-8051(97)80010-8","volume":"24","author":"P Winnard Jr.","year":"1997","unstructured":"Winnard, P. Jr., Chang, F., Rusckowski, M., Mardirossian, G. & Hnatowich, D.J. Preparation and use of NHS-MAG3 for technetium-99m labeling of DNA. Nucl. Med. Biol. 24, 425\u2013432 (1997).","journal-title":"Nucl. Med. Biol."}],"container-title":["Nature Protocols"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nprot.2007.144.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nprot.2007.144","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nprot.2007.144.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T20:05:17Z","timestamp":1684440317000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nprot.2007.144"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4]]},"references-count":14,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,4]]}},"alternative-id":["BFnprot2007144"],"URL":"https:\/\/doi.org\/10.1038\/nprot.2007.144","relation":{},"ISSN":["1754-2189","1750-2799"],"issn-type":[{"value":"1754-2189","type":"print"},{"value":"1750-2799","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4]]}}}