{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:50:40Z","timestamp":1791341440140,"version":"4.3.1"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"9","license":[{"start":{"date-parts":[[2007,8,23]],"date-time":"2007-08-23T00:00:00Z","timestamp":1187827200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nat Protoc"],"published-print":{"date-parts":[[2007,9]]},"DOI":"10.1038\/nprot.2007.296","type":"journal-article","created":{"date-parts":[[2007,8,23]],"date-time":"2007-08-23T13:26:28Z","timestamp":1187875588000},"page":"2049-2056","source":"Crossref","is-referenced-by-count":529,"title":["Monitoring lymphocyte proliferation in vitro and in vivo with the intracellular fluorescent dye carboxyfluorescein diacetate succinimidyl ester"],"prefix":"10.1038","volume":"2","author":[{"given":"Ben J C","family":"Quah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hilary S","family":"Warren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher R","family":"Parish","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2007,8,23]]},"reference":[{"key":"BFnprot2007296_CR1","doi-asserted-by":"publisher","first-page":"499","DOI":"10.1046\/j.1440-1711.1999.00877.x","volume":"77","author":"CR Parish","year":"1999","unstructured":"Parish, C.R. Fluorescent dyes for lymphocyte migration and proliferation studies. Immunol. Cell Biol. 77, 499\u2013508 (1999).","journal-title":"Immunol. Cell Biol."},{"key":"BFnprot2007296_CR2","doi-asserted-by":"publisher","first-page":"516","DOI":"10.1046\/j.1440-1711.1999.00874.x","volume":"77","author":"J Hasbold","year":"1999","unstructured":"Hasbold, J. et al. Quantitative analysis of lymphocyte differentiation and proliferation in vitro using carboxyfluorescein diacetate succinimidyl ester. Immunol. Cell Biol. 77, 516\u2013522 (1999).","journal-title":"Immunol. Cell Biol."},{"key":"BFnprot2007296_CR3","doi-asserted-by":"publisher","first-page":"544","DOI":"10.1046\/j.1440-1711.1999.00865.x","volume":"77","author":"HS Warren","year":"1999","unstructured":"Warren, H.S. Using carboxyfluorescein diacetate succinimidyl ester to monitor human NK cell division: analysis of the effect of activating and inhibitory class I MHC receptors. Immunol. Cell Biol. 77, 544\u2013551 (1999).","journal-title":"Immunol. Cell Biol."},{"key":"BFnprot2007296_CR4","doi-asserted-by":"publisher","first-page":"147","DOI":"10.1016\/S0022-1759(00)00231-3","volume":"243","author":"AB Lyons","year":"2000","unstructured":"Lyons, A.B. Analysing cell division in vivo and in vitro using flow cytometric measurement of CFSE dye dilution. J. Immunol. Methods 243, 147\u2013154 (2000).","journal-title":"J. Immunol. Methods"},{"key":"BFnprot2007296_CR5","first-page":"1","volume":"4.9","author":"CR Parish","year":"2001","unstructured":"Parish, C.R. & Warren, H.S. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Curr. Protoc. Immunol. 4.9, 1\u201310 (2001).","journal-title":"Curr. Protoc. Immunol."},{"key":"BFnprot2007296_CR6","doi-asserted-by":"publisher","first-page":"131","DOI":"10.1016\/0022-1759(94)90236-4","volume":"171","author":"AB Lyons","year":"1994","unstructured":"Lyons, A.B. & Parish, C.R. Determination of lymphocyte division by flow cytometry. J. Immunol. Methods 171, 131\u2013137 (1994).","journal-title":"J. Immunol. Methods"},{"key":"BFnprot2007296_CR7","doi-asserted-by":"publisher","first-page":"87","DOI":"10.1016\/0022-1759(90)90322-M","volume":"133","author":"SA Weston","year":"1990","unstructured":"Weston, S.A. & Parish, C.R. New fluorescent dyes for lymphocyte migration studies. Analysis by flow cytometry and fluorescence microscopy. J. Immunol. Methods 133, 87\u201397 (1990).","journal-title":"J. Immunol. Methods"},{"key":"BFnprot2007296_CR8","doi-asserted-by":"publisher","first-page":"6047","DOI":"10.4049\/jimmunol.165.11.6047","volume":"165","author":"AL Marzo","year":"2000","unstructured":"Marzo, A.L. et al. Tumor-specific CD4+ T cells have a major 'post-licensing' role in CTL mediated anti-tumor immunity. J. Immunol. 165, 6047\u20136055 (2000).","journal-title":"J. Immunol."},{"key":"BFnprot2007296_CR9","doi-asserted-by":"publisher","first-page":"2677","DOI":"10.1182\/blood-2003-06-2070","volume":"103","author":"I Jedema","year":"2004","unstructured":"Jedema, I., van der Werff, N.M., Barge, R.M., Willemze, R. & Falkenburg, J.H. New CFSE-based assay to determine susceptibility to lysis by cytotoxic T cells of leukemic precursor cells within a heterogeneous target cell population. Blood 103, 2677\u20132682 (2004).","journal-title":"Blood"},{"key":"BFnprot2007296_CR10","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.jim.2003.10.017","volume":"285","author":"IF Hermans","year":"2004","unstructured":"Hermans, I.F. et al. The VITAL assay: a versatile fluorometric technique for assessing CTL- and NKT-mediated cytotoxicity against multiple targets in vitro and in vivo. J. Immunol. Methods 285, 25\u201340 (2004).","journal-title":"J. Immunol. Methods"},{"key":"BFnprot2007296_CR11","doi-asserted-by":"publisher","first-page":"1285","DOI":"10.4049\/jimmunol.178.3.1285","volume":"178","author":"J Stambas","year":"2007","unstructured":"Stambas, J., Doherty, P.C. & Turner, S.J. An in vivo cytotoxicity threshold for influenza A virus-specific effector and memory CD8(+) T cells. J. Immunol. 178, 1285\u20131292 (2007).","journal-title":"J. Immunol."},{"key":"BFnprot2007296_CR12","doi-asserted-by":"publisher","first-page":"277","DOI":"10.1084\/jem.184.1.277","volume":"184","author":"PD Hodgkin","year":"1996","unstructured":"Hodgkin, P.D., Lee, J.H. & Lyons, A.B. B cell differentiation and isotype switching is related to division cycle number. J. Exp. Med. 184, 277\u2013281 (1996).","journal-title":"J. Exp. Med."},{"key":"BFnprot2007296_CR13","doi-asserted-by":"publisher","first-page":"9488","DOI":"10.1073\/pnas.95.16.9488","volume":"95","author":"AV Gett","year":"1998","unstructured":"Gett, A.V. & Hodgkin, P.D. Cell division regulates the T cell cytokine repertoire, revealing a mechanism underlying immune class regulation. Proc. Natl. Acad. Sci. USA 95, 9488\u20139493 (1998).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnprot2007296_CR14","doi-asserted-by":"publisher","first-page":"229","DOI":"10.1016\/S1074-7613(00)80605-6","volume":"9","author":"JJ Bird","year":"1998","unstructured":"Bird, J.J. et al. Helper T cell differentiation is controlled by the cell cycle. Immunity 9, 229\u2013237 (1998).","journal-title":"Immunity"},{"key":"BFnprot2007296_CR15","doi-asserted-by":"publisher","first-page":"530","DOI":"10.1046\/j.1440-1711.1999.00871.x","volume":"77","author":"B Fazekas de St Groth","year":"1999","unstructured":"Fazekas de St Groth, B. Carboxyfluorescein diacetate succinimidyl ester and the virgin lymphocyte: a marriage made in heaven. Immunol. Cell Biol. 77, 530\u2013538 (1999).","journal-title":"Immunol. Cell Biol."},{"key":"BFnprot2007296_CR16","doi-asserted-by":"publisher","first-page":"523","DOI":"10.1046\/j.1440-1711.1999.00869.x","volume":"77","author":"RE Nordon","year":"1999","unstructured":"Nordon, R.E., Nakamura, M., Ramirez, C. & Odell, R. Analysis of growth kinetics by division tracking. Immunol. Cell Biol. 77, 523\u2013529 (1999).","journal-title":"Immunol. Cell Biol."},{"key":"BFnprot2007296_CR17","doi-asserted-by":"publisher","first-page":"239","DOI":"10.1038\/79782","volume":"1","author":"AV Gett","year":"2000","unstructured":"Gett, A.V. & Hodgkin, P.D. A cellular calculus for signal integration by T cells. Nat. Immunol. 1, 239\u2013244 (2000).","journal-title":"Nat. Immunol."},{"key":"BFnprot2007296_CR18","doi-asserted-by":"publisher","first-page":"1011","DOI":"10.1007\/s11538-006-9094-8","volume":"68","author":"RJ De Boer","year":"2006","unstructured":"De Boer, R.J., Ganusov, V.V., Milutinovic, D., Hodgkin, P.D. & Perelson, A.S. Estimating lymphocyte division and death rates from CFSE data. Bull. Math. Biol. 68, 1011\u20131031 (2006).","journal-title":"Bull. Math. Biol."},{"key":"BFnprot2007296_CR19","doi-asserted-by":"publisher","first-page":"119","DOI":"10.1111\/j.1600-065X.2006.00498.x","volume":"216","author":"R Callard","year":"2007","unstructured":"Callard, R. & Hodgkin, P. Modeling T- and B-cell growth and differentiation. Immunol. Rev. 216, 119\u2013129 (2007).","journal-title":"Immunol. Rev."},{"key":"BFnprot2007296_CR20","doi-asserted-by":"publisher","first-page":"2057","DOI":"10.1038\/nprot.2007.297","volume":"2","author":"E Hawkins","year":"2007","unstructured":"Hawkins, E. et al. Measuring lymphocyte proliferation, survival and differentiation using CFSE time series data. Nat. Protoc. 2, 2057\u20132067 (2007).","journal-title":"Nat. Protoc."},{"key":"BFnprot2007296_CR21","doi-asserted-by":"publisher","first-page":"295","DOI":"10.1046\/j.1472-765X.1997.00225.x","volume":"25","author":"JE Ueckert","year":"1997","unstructured":"Ueckert, J.E., Nebe von-Caron, G., Bos, A.P. & ter Steeg, P.F. Flow cytometric analysis of Lactobacillus plantarum to monitor lag times, cell division and injury. Lett. Appl. Microbiol. 25, 295\u2013299 (1997).","journal-title":"Lett. Appl. Microbiol."},{"key":"BFnprot2007296_CR22","doi-asserted-by":"crossref","first-page":"742","DOI":"10.1016\/S1016-8478(23)13373-5","volume":"7","author":"LY Khil","year":"1997","unstructured":"Khil, L.Y. et al. Insulin has a limited effect on the cell cycle progression in 3T3 L1 fibroblasts. Mol. Cells 7, 742\u2013748 (1997).","journal-title":"Mol. Cells"},{"key":"BFnprot2007296_CR23","doi-asserted-by":"crossref","first-page":"855","DOI":"10.1182\/blood.V95.3.855.003k41_855_862","volume":"95","author":"RA Oostendorp","year":"2000","unstructured":"Oostendorp, R.A., Audet, J. & Eaves, C.J. High-resolution tracking of cell division suggests similar cell cycle kinetics of hematopoietic stem cells stimulated in vitro and in vivo. Blood 95, 855\u2013862 (2000).","journal-title":"Blood"},{"key":"BFnprot2007296_CR24","doi-asserted-by":"publisher","first-page":"471","DOI":"10.1111\/j.0818-9641.2004.01275.x","volume":"82","author":"MB Sukkar","year":"2004","unstructured":"Sukkar, M.B. et al. 'Proliferative' and 'synthetic' airway smooth muscle cells are overlapping populations. Immunol. Cell Biol. 82, 471\u2013478 (2004).","journal-title":"Immunol. Cell Biol."},{"key":"BFnprot2007296_CR25","doi-asserted-by":"crossref","first-page":"4171","DOI":"10.4049\/jimmunol.158.9.4171","volume":"158","author":"AE Macaulay","year":"1997","unstructured":"Macaulay, A.E., DeKruyff, R.H., Goodnow, C.C. & Umetsu, D.T. Antigen-specific B cells preferentially induce CD4+ T cells to produce IL-4. J. Immunol. 158, 4171\u20134179 (1997).","journal-title":"J. Immunol."}],"container-title":["Nature Protocols"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nprot.2007.296.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nprot.2007.296","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nprot.2007.296.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,17]],"date-time":"2024-02-17T10:20:04Z","timestamp":1708165204000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nprot.2007.296"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8,23]]},"references-count":25,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["BFnprot2007296"],"URL":"https:\/\/doi.org\/10.1038\/nprot.2007.296","relation":{},"ISSN":["1754-2189","1750-2799"],"issn-type":[{"value":"1754-2189","type":"print"},{"value":"1750-2799","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,8,23]]}}}