{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T05:59:39Z","timestamp":1792735179015,"version":"4.5.0"},"reference-count":25,"publisher":"Springer Science and Business Media LLC","issue":"10","license":[{"start":{"date-parts":[[2007,10,1]],"date-time":"2007-10-01T00:00:00Z","timestamp":1191196800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nat Protoc"],"published-print":{"date-parts":[[2007,10]]},"DOI":"10.1038\/nprot.2007.368","type":"journal-article","created":{"date-parts":[[2007,10,11]],"date-time":"2007-10-11T16:57:03Z","timestamp":1192121823000},"page":"2545-2550","source":"Crossref","is-referenced-by-count":96,"title":["The mouse cornea micropocket angiogenesis assay"],"prefix":"10.1038","volume":"2","author":[{"given":"Michael S","family":"Rogers","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy E","family":"Birsner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert J","family":"D'Amato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2007,10,11]]},"reference":[{"key":"BFnprot2007368_CR1","doi-asserted-by":"publisher","first-page":"4120","DOI":"10.1073\/pnas.73.11.4120","volume":"73","author":"D Gospodarowicz","year":"1976","unstructured":"Gospodarowicz, D., Moran, J., Braun, D. & Birdwell, C. Clonal growth of bovine vascular endothelial cells: fibroblast growth factor as a survival agent. Proc. Natl. Acad. Sci. USA 73, 4120\u20134124 (1976).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnprot2007368_CR2","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1038\/288483a0","volume":"288","author":"BM Glaser","year":"1980","unstructured":"Glaser, B.M., D'Amore, P.A., Seppa, H., Seppa, S. & Schiffmann, E. Adult tissues contain chemoattractants for vascular endothelial cells. Nature 288, 483\u2013484 (1980).","journal-title":"Nature"},{"key":"BFnprot2007368_CR3","doi-asserted-by":"publisher","first-page":"1589","DOI":"10.1083\/jcb.107.4.1589","volume":"107","author":"Y Kubota","year":"1988","unstructured":"Kubota, Y., Kleinman, H.K., Martin, G.R. & Lawley, T.J. Role of laminin and basement membrane in the morphological differentiation of human endothelial cells into capillary-like structures. J. Cell Biol. 107, 1589\u20131598 (1988).","journal-title":"J. Cell Biol."},{"key":"BFnprot2007368_CR4","doi-asserted-by":"publisher","first-page":"469","DOI":"10.1016\/0092-8674(85)90104-7","volume":"42","author":"R Montesano","year":"1985","unstructured":"Montesano, R. & Orci, L. Tumor-promoting phorbol esters induce angiogenesis in vitro. Cell 42, 469\u2013477 (1985).","journal-title":"Cell"},{"key":"BFnprot2007368_CR5","doi-asserted-by":"publisher","first-page":"1341","DOI":"10.1083\/jcb.143.5.1341","volume":"143","author":"T Korff","year":"1998","unstructured":"Korff, T. & Augustin, H.G. Integration of endothelial cells in multicellular spheroids prevents apoptosis and induces differentiation. J. Cell Biol. 143, 1341\u20131352 (1998).","journal-title":"J. Cell Biol."},{"key":"BFnprot2007368_CR6","doi-asserted-by":"publisher","first-page":"538","DOI":"10.1007\/BF02810077","volume":"18","author":"RF Nicosia","year":"1982","unstructured":"Nicosia, R.F., Tchao, R. & Leighton, J. Histotypic angiogenesis in vitro: light microscopic, ultrastructural, and radioautographic studies. In Vitro 18, 538\u2013549 (1982).","journal-title":"In Vitro"},{"key":"BFnprot2007368_CR7","doi-asserted-by":"publisher","first-page":"281","DOI":"10.1002\/ar.1090280403","volume":"28","author":"JC Sandison","year":"1924","unstructured":"Sandison, J.C. A new method for the microscopic study of living growing tissues by the introduction of a transparent chamber in the rabbit's ear. Anat. Rec. 28, 281\u2013287 (1924).","journal-title":"Anat. Rec."},{"key":"BFnprot2007368_CR8","doi-asserted-by":"publisher","first-page":"1203","DOI":"10.1038\/nm1197-1203","volume":"3","author":"RK Jain","year":"1997","unstructured":"Jain, R.K., Schlenger, K., Hockel, M. & Yuan, F. Quantitative angiogenesis assays: progress and problems. Nat. Med. 3, 1203\u20131208 (1997).","journal-title":"Nat. Med."},{"key":"BFnprot2007368_CR9","doi-asserted-by":"publisher","first-page":"261","DOI":"10.1084\/jem.136.2.261","volume":"136","author":"MA Gimbrone","year":"1972","unstructured":"Gimbrone, M.A., Leapman, S.B., Cotran, R.S. & Folkman, J. Tumor dormancy in vivo by prevention of neovascularization. J. Exp. Med. 136, 261\u2013276 (1972).","journal-title":"J. Exp. Med."},{"key":"BFnprot2007368_CR10","first-page":"4409","volume":"38","author":"A Maiorana","year":"1978","unstructured":"Maiorana, A. & Gullino, P.M. Acquisition of angiogenic capacity and neoplastic transformation in the rat mammary gland. Cancer Res. 38, 4409\u20134414 (1978).","journal-title":"Cancer Res."},{"key":"BFnprot2007368_CR11","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1093\/jnci\/52.2.413","volume":"52","author":"MA Gimbrone","year":"1974","unstructured":"Gimbrone, M.A., Cotran, R.S., Leapman, S.B. & Folkman, J. Tumor growth and neovascularization: an experimental model using the rabbit cornea. J. Natl. Cancer Inst. 52, 413\u2013427 (1974).","journal-title":"J. Natl. Cancer Inst."},{"key":"BFnprot2007368_CR12","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1093\/jnci\/50.1.219","volume":"50","author":"MA Gimbrone","year":"1973","unstructured":"Gimbrone, M.A., Leapman, S.B., Cotran, R.S. & Folkman, J. Tumor angiogenesis: iris neovascularization at a distance from experimental intraocular tumors. J. Natl. Cancer Inst. 50, 219\u2013228 (1973).","journal-title":"J. Natl. Cancer Inst."},{"key":"BFnprot2007368_CR13","doi-asserted-by":"publisher","first-page":"1416","DOI":"10.1126\/science.472760","volume":"205","author":"V Muthukkaruppan","year":"1979","unstructured":"Muthukkaruppan, V. & Auerbach, R. Angiogenesis in the mouse cornea. Science 205, 1416\u20131418 (1979).","journal-title":"Science"},{"key":"BFnprot2007368_CR14","doi-asserted-by":"publisher","first-page":"4082","DOI":"10.1073\/pnas.91.9.4082","volume":"91","author":"RJ D'Amato","year":"1994","unstructured":"D'Amato, R.J., Loughnan, M.S., Flynn, E. & Folkman, J. Thalidomide is an inhibitor of angiogenesis. Proc. Natl. Acad. Sci. USA 91, 4082\u20134085 (1994).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnprot2007368_CR15","first-page":"2906","volume":"32","author":"WW Li","year":"1991","unstructured":"Li, W.W., Grayson, G., Folkman, J. & D'Amore, P.A. Sustained-release endotoxin. A model for inducing corneal neovascularization. Invest. Ophthalmol. Vis. Sci. 32, 2906\u20132911 (1991).","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"BFnprot2007368_CR16","first-page":"961","volume":"8","author":"GD Phillips","year":"1994","unstructured":"Phillips, G.D. et al. Vascular endothelial growth factor (rhVEGF165) stimulates direct angiogenesis in the rabbit cornea. In Vivo 8, 961\u2013965 (1994).","journal-title":"In Vivo"},{"key":"BFnprot2007368_CR17","doi-asserted-by":"publisher","first-page":"573","DOI":"10.1001\/archopht.1990.01070060121061","volume":"108","author":"D BenEzra","year":"1990","unstructured":"BenEzra, D., Hemo, I. & Maftzir, G. In vivo angiogenic activity of interleukins. Arch. Ophthalmol. 108, 573\u2013576 (1990).","journal-title":"Arch. Ophthalmol."},{"key":"BFnprot2007368_CR18","doi-asserted-by":"publisher","first-page":"S134","DOI":"10.1097\/00005373-199012001-00027","volume":"30","author":"DR Knighton","year":"1990","unstructured":"Knighton, D.R., Phillips, G.D. & Fiegel, V.D. Wound healing angiogenesis: indirect stimulation by basic fibroblast growth factor. J. Trauma 30, S134\u2013S144 (1990).","journal-title":"J. Trauma"},{"key":"BFnprot2007368_CR19","first-page":"1625","volume":"37","author":"BM Kenyon","year":"1996","unstructured":"Kenyon, B.M. et al. A model of angiogenesis in the mouse cornea. Invest. Ophthalmol. Vis. Sci. 37, 1625\u20131632 (1996).","journal-title":"Invest. Ophthalmol. Vis. Sci."},{"key":"BFnprot2007368_CR20","doi-asserted-by":"publisher","first-page":"11658","DOI":"10.1073\/pnas.0404272101","volume":"101","author":"LK Chang","year":"2004","unstructured":"Chang, L.K. et al. Dose-dependent response of FGF-2 for lymphangiogenesis. Proc. Natl. Acad. Sci. USA 101, 11658\u201311663 (2004).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnprot2007368_CR21","doi-asserted-by":"publisher","first-page":"1874","DOI":"10.1073\/pnas.0308525100","volume":"101","author":"MV Hoang","year":"2004","unstructured":"Hoang, M.V., Whelan, M.C. & Senger, D.R. Rho activity critically and selectively regulates endothelial cell organization during angiogenesis. Proc. Natl. Acad. Sci. USA 101, 1874\u20131879 (2004).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnprot2007368_CR22","doi-asserted-by":"publisher","first-page":"2112","DOI":"10.1096\/fj.03-0246fje","volume":"17","author":"MS Rogers","year":"2003","unstructured":"Rogers, M.S., Rohan, R.M., Birsner, A.E. & D'Amato, R.J. Genetic loci that control vascular endothelial growth factor-induced angiogenesis. FASEB J. 17, 2112\u20132114 (2003).","journal-title":"FASEB J."},{"key":"BFnprot2007368_CR23","doi-asserted-by":"publisher","first-page":"561","DOI":"10.1016\/j.yexcr.2005.10.021","volume":"312","author":"MS Rogers","year":"2006","unstructured":"Rogers, M.S. & D'Amato, R.J. The effect of genetic diversity on angiogenesis. Exp. Cell Res. 312, 561\u2013574 (2006).","journal-title":"Exp. Cell Res."},{"key":"BFnprot2007368_CR24","doi-asserted-by":"publisher","first-page":"1050","DOI":"10.1096\/fj.03-1241com","volume":"18","author":"MS Rogers","year":"2004","unstructured":"Rogers, M.S., Rohan, R.M., Birsner, A.E. & D'Amato, R.J. Genetic loci that control the angiogenic response to basic fibroblast growth factor. FASEB J. 18, 1050\u20131059 (2004).","journal-title":"FASEB J."},{"key":"BFnprot2007368_CR25","doi-asserted-by":"publisher","first-page":"871","DOI":"10.1096\/fasebj.14.7.871","volume":"14","author":"RM Rohan","year":"2000","unstructured":"Rohan, R.M., Fernandez, A., Udagawa, T., Yuan, J. & D'Amato, R.J. Genetic heterogeneity of angiogenesis in mice. FASEB J. 14, 871\u2013876 (2000).","journal-title":"FASEB J."}],"container-title":["Nature Protocols"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nprot.2007.368.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nprot.2007.368","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nprot.2007.368.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T00:07:01Z","timestamp":1684454821000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nprot.2007.368"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,10]]},"references-count":25,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2007,10]]}},"alternative-id":["BFnprot2007368"],"URL":"https:\/\/doi.org\/10.1038\/nprot.2007.368","relation":{},"ISSN":["1754-2189","1750-2799"],"issn-type":[{"value":"1754-2189","type":"print"},{"value":"1750-2799","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,10]]}}}