{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T10:32:17Z","timestamp":1792665137464,"version":"4.5.0"},"reference-count":56,"publisher":"Springer Science and Business Media LLC","issue":"6","license":[{"start":{"date-parts":[[2008,5,15]],"date-time":"2008-05-15T00:00:00Z","timestamp":1210809600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nat Protoc"],"published-print":{"date-parts":[[2008,6]]},"DOI":"10.1038\/nprot.2008.61","type":"journal-article","created":{"date-parts":[[2008,5,15]],"date-time":"2008-05-15T12:01:21Z","timestamp":1210852881000},"page":"965-976","source":"Crossref","is-referenced-by-count":769,"title":["Analysis of mitochondrial function in situ in permeabilized muscle fibers, tissues and cells"],"prefix":"10.1038","volume":"3","author":[{"given":"Andrey V","family":"Kuznetsov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Veksler","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank N","family":"Gellerich","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Valdur","family":"Saks","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raimund","family":"Margreiter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfram S","family":"Kunz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2008,5,15]]},"reference":[{"key":"BFnprot200861_CR1","doi-asserted-by":"publisher","first-page":"481","DOI":"10.1016\/S0092-8674(03)00116-8","volume":"112","author":"DD Newmeyer","year":"2003","unstructured":"Newmeyer, D.D. & Ferguson-Miller, S. Mitochondria: releasing power for life and unleashing the machineries of death. Cell 112, 481\u2013490 (2003).","journal-title":"Cell"},{"key":"BFnprot200861_CR2","doi-asserted-by":"publisher","first-page":"513","DOI":"10.1038\/74994","volume":"6","author":"G Kroemer","year":"2000","unstructured":"Kroemer, G. & Reed, J.C. Mitochondrial control of cell death. Nat. Med. 6, 513\u2013519 (2000).","journal-title":"Nat. Med."},{"key":"BFnprot200861_CR3","doi-asserted-by":"publisher","first-page":"1175","DOI":"10.1146\/annurev.bi.61.070192.005523","volume":"61","author":"DC Wallace","year":"1992","unstructured":"Wallace, D.C. Diseases of the mitochondrial DNA. Annu. Rev. Biochem. 61, 1175\u20131212 (1992).","journal-title":"Annu. Rev. Biochem."},{"key":"BFnprot200861_CR4","doi-asserted-by":"publisher","first-page":"1482","DOI":"10.1126\/science.283.5407.1482","volume":"283","author":"DC Wallace","year":"1999","unstructured":"Wallace, D.C. Mitochondrial diseases in man and mouse. Science 283, 1482\u20131488 (1999).","journal-title":"Science"},{"key":"BFnprot200861_CR5","doi-asserted-by":"publisher","first-page":"787","DOI":"10.1038\/nature05292","volume":"443","author":"MT Lin","year":"2006","unstructured":"Lin, M.T. & Beal, M.F. Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. Nature 443, 787\u2013795 (2006).","journal-title":"Nature"},{"key":"BFnprot200861_CR6","doi-asserted-by":"publisher","first-page":"1261","DOI":"10.1038\/sj.cdd.4402160","volume":"14","author":"AH Schapira","year":"2007","unstructured":"Schapira, A.H. Mitochondrial dysfunction in Parkinson's disease. Cell Death Differ. 14, 1261\u20131266 (2007).","journal-title":"Cell Death Differ."},{"key":"BFnprot200861_CR7","doi-asserted-by":"publisher","first-page":"215","DOI":"10.1038\/ng1097-215","volume":"17","author":"A R\u00f6tig","year":"1997","unstructured":"R\u00f6tig, A. et al. Aconitase and mitochondrial iron-sulphur protein deficiency in Friedreich ataxia. Nat. Genet. 17, 215\u2013217 (1997).","journal-title":"Nat. Genet."},{"key":"BFnprot200861_CR8","doi-asserted-by":"publisher","first-page":"1339","DOI":"10.1093\/brain\/123.7.1339","volume":"123","author":"S Vielhaber","year":"2000","unstructured":"Vielhaber, S. et al. Mitochondrial DNA abnormalities in skeletal muscle of patients with sporadic amyotrophic lateral sclerosis. Brain 123, 1339\u20131348 (2000).","journal-title":"Brain"},{"key":"BFnprot200861_CR9","doi-asserted-by":"publisher","first-page":"193","DOI":"10.1038\/nrc1013","volume":"3","author":"C Eng","year":"2003","unstructured":"Eng, C., Kiuru, M., Fernandez, M.J. & Aaltonen, L.A. A role for mitochondrial enzymes in inherited neoplasia and beyond. Nat. Rev. Cancer 3, 193\u2013202 (2003).","journal-title":"Nat. Rev. Cancer"},{"key":"BFnprot200861_CR10","doi-asserted-by":"publisher","first-page":"483","DOI":"10.1016\/j.cell.2005.02.001","volume":"120","author":"RS Balaban","year":"2005","unstructured":"Balaban, R.S., Nemoto, S. & Finkel, T. Mitochondria, oxidants, and aging. Cell 120, 483\u201395 (2005).","journal-title":"Cell"},{"key":"BFnprot200861_CR11","doi-asserted-by":"publisher","first-page":"287","DOI":"10.1038\/nprot.2006.478","volume":"2","author":"C Frezza","year":"2007","unstructured":"Frezza, C., Cipolat, S. & Scorrano, L. Organelle isolation: functional mitochondria from mouse liver, muscle and cultured fibroblasts. Nat. Protoc. 2, 287\u2013295 (2007).","journal-title":"Nat. Protoc."},{"key":"BFnprot200861_CR12","doi-asserted-by":"publisher","first-page":"81","DOI":"10.1023\/A:1006834912257","volume":"184","author":"VA Saks","year":"1998","unstructured":"Saks, V.A. et al. Permeabilized cell and skinned fiber techniques in studies of mitochondrial function in vivo. Mol. Cell. Biochem. 184, 81\u2013100 (1998).","journal-title":"Mol. Cell. Biochem."},{"key":"BFnprot200861_CR13","doi-asserted-by":"publisher","first-page":"1283","DOI":"10.1083\/jcb.150.6.1283","volume":"150","author":"DJ Milner","year":"2000","unstructured":"Milner, D.J., Mavroidis, M., Weisleder, N. & Capetanaki, Y. Desmin cytoskeleton linked to muscle mitochondrial distribution and respiratory function. J. Cell. Biol. 150, 1283\u20131298 (2000).","journal-title":"J. Cell. Biol."},{"key":"BFnprot200861_CR14","doi-asserted-by":"publisher","first-page":"27741","DOI":"10.1074\/jbc.M007296200","volume":"275","author":"WS Kunz","year":"2000","unstructured":"Kunz, W.S. et al. Flux control of cytochrome c oxidase in human skeletal muscle. J. Biol. Chem. 275, 27741\u201327745 (2000).","journal-title":"J. Biol. Chem."},{"key":"BFnprot200861_CR15","doi-asserted-by":"publisher","first-page":"31829","DOI":"10.1074\/jbc.273.48.31829","volume":"273","author":"G Villani","year":"1998","unstructured":"Villani, G., Greco, M., Papa, S. & Attardi, G. Low reserve of cytochrome c oxidase capacity in vivo in the respiratory chain of a variety of human cell types. J. Biol. Chem. 273, 31829\u201331836 (1998).","journal-title":"J. Biol. Chem."},{"key":"BFnprot200861_CR16","doi-asserted-by":"publisher","first-page":"885","DOI":"10.1016\/S0022-2828(85)80102-4","volume":"17","author":"HM Piper","year":"1985","unstructured":"Piper, H.M. et al. Development of ischemia-induced damage in defined mitochondrial subpopulations. J. Mol. Cell. Cardiol. 17, 885\u2013896 (1985).","journal-title":"J. Mol. Cell. Cardiol."},{"key":"BFnprot200861_CR17","doi-asserted-by":"publisher","first-page":"450","DOI":"10.1016\/S0168-9525(97)01266-3","volume":"13","author":"RN Lightowlers","year":"1997","unstructured":"Lightowlers, R.N., Chinnery, P.F., Turnbull, D.M. & Howell, N. Mammalian mitochondrial genetics: heredity, heteroplasmy and disease. Trends Genet. 13, 450\u2013455 (1997).","journal-title":"Trends Genet."},{"key":"BFnprot200861_CR18","doi-asserted-by":"publisher","first-page":"6937","DOI":"10.1074\/jbc.275.10.6937","volume":"275","author":"L Kay","year":"2000","unstructured":"Kay, L., Nicolay, K., Wieringa, B., Saks, V. & Wallimann, T. Direct evidence for the control of mitochondrial respiration by mitochondrial creatine kinase in oxidative muscle cells in situ. J. Biol. Chem. 275, 6937\u20136944 (2000).","journal-title":"J. Biol. Chem."},{"key":"BFnprot200861_CR19","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1016\/0304-4165(91)90168-G","volume":"1074","author":"VA Saks","year":"1991","unstructured":"Saks, V.A., Belikova, Y.O. & Kuznetsov, A.V. In vivo regulation of mitochondrial respiration in cardiomyocytes: specific restrictions for intracellular diffusion of ADP. Biochim. Biophys. Acta 1074, 302\u2013311 (1991).","journal-title":"Biochim. Biophys. Acta"},{"key":"BFnprot200861_CR20","doi-asserted-by":"publisher","first-page":"1091","DOI":"10.1083\/jcb.140.5.1091","volume":"140","author":"AV Kuznetsov","year":"1998","unstructured":"Kuznetsov, A.V. et al. Functional imaging of mitochondria in saponin-permeabilized mice muscle fibers. J. Cell Biol. 140, 1091\u20131099 (1998).","journal-title":"J. Cell Biol."},{"key":"BFnprot200861_CR21","doi-asserted-by":"publisher","first-page":"686","DOI":"10.1016\/j.bbabio.2006.03.014","volume":"1757","author":"AV Kuznetsov","year":"2006","unstructured":"Kuznetsov, A.V. et al. Mitochondrial subpopulations and heterogeneity revealed by confocal imaging: possible physiological role? Biochim. Biophys. Acta 1757, 686\u2013691 (2006).","journal-title":"Biochim. Biophys. Acta"},{"key":"BFnprot200861_CR22","doi-asserted-by":"publisher","first-page":"1241","DOI":"10.1016\/j.cell.2006.06.010","volume":"125","author":"DC Chan","year":"2006","unstructured":"Chan, D.C. Mitochondria: dynamic organelles in disease, aging, and development. Cell 125, 1241\u20131252 (2006).","journal-title":"Cell"},{"key":"BFnprot200861_CR23","doi-asserted-by":"crossref","first-page":"14431","DOI":"10.1016\/S0021-9258(18)98703-X","volume":"266","author":"AE Vercesi","year":"1991","unstructured":"Vercesi, A.E., Bernardes, C.F., Hoffmann, M.E., Gadelha, F.R. & Docampo, R. Digitonin permeabilization does not affect mitochondrial function and allows the determination of the mitochondrial membrane potential of Trypanosoma cruzi in situ. J. Biol. Chem. 266, 14431\u201314434 (1991).","journal-title":"J. Biol. Chem."},{"key":"BFnprot200861_CR24","doi-asserted-by":"publisher","first-page":"191","DOI":"10.1016\/0005-2728(87)90174-5","volume":"892","author":"VI Veksler","year":"1987","unstructured":"Veksler, V.I., Kuznetsov, A.V., Sharov, V.G., Kapelko, V.I. & Saks, V.A. Mitochondrial respiratory parameters in cardiac tissue: a novel method of assessment by using saponin-skinned fibers. Biochim. Biophys. Acta 892, 191\u2013196 (1987).","journal-title":"Biochim. Biophys. Acta"},{"key":"BFnprot200861_CR25","doi-asserted-by":"publisher","first-page":"46","DOI":"10.1016\/0005-2728(93)90029-F","volume":"1144","author":"WS Kunz","year":"1993","unstructured":"Kunz, W.S. et al. Functional characterization of mitochondrial oxidative phosphorylation in saponin-skinned human muscle fibers. Biochim. Biophys. Acta 1144, 46\u201353 (1993).","journal-title":"Biochim. Biophys. Acta"},{"key":"BFnprot200861_CR26","doi-asserted-by":"publisher","first-page":"17","DOI":"10.1203\/00006450-199207000-00004","volume":"32","author":"T Letellier","year":"1992","unstructured":"Letellier, T. et al. Mitochondrial myopathy studies on permeabilized muscle fibers. Pediatr. Res. 32, 17\u201322 (1992).","journal-title":"Pediatr. Res."},{"key":"BFnprot200861_CR27","doi-asserted-by":"publisher","first-page":"1580","DOI":"10.1046\/j.1471-4159.1999.721580.x","volume":"72","author":"WS Kunz","year":"1999","unstructured":"Kunz, W.S., Kuznetsov, A.V., Clark, J.F., Tracey, I. & Elger, C.E. Metabolic consequences of the cytochrome c oxidase deficiency in brain of copper-deficient Mo(vbr) mice. J. Neurochem. 72, 1580\u20131585 (1999).","journal-title":"J. Neurochem."},{"key":"BFnprot200861_CR28","doi-asserted-by":"publisher","first-page":"186","DOI":"10.1006\/abio.2002.5658","volume":"305","author":"AV Kuznetsov","year":"2002","unstructured":"Kuznetsov, A.V. et al. Evaluation of mitochondrial respiratory function in small biopsies of liver. Anal. Biochem. 305, 186\u2013194 (2002).","journal-title":"Anal. Biochem."},{"key":"BFnprot200861_CR29","doi-asserted-by":"publisher","first-page":"263","DOI":"10.1146\/annurev.bi.38.070169.001403","volume":"38","author":"E Korn","year":"1969","unstructured":"Korn, E. Cell membranes: structure and synthesis. Annu. Rev. Biochem. 38, 263\u2013288 (1969).","journal-title":"Annu. Rev. Biochem."},{"key":"BFnprot200861_CR30","doi-asserted-by":"publisher","first-page":"271","DOI":"10.1016\/0005-2736(76)90353-9","volume":"419","author":"J Comte","year":"1976","unstructured":"Comte, J., Maisterrena, B. & Gautheron, D.C . Lipid composition and protein profiles of outer and inner membranes from pig heart mitochondria comparison with microsomes. Biochim. Biophys. Acta 419, 271\u2013284 (1976).","journal-title":"Biochim. Biophys. Acta"},{"key":"BFnprot200861_CR31","doi-asserted-by":"publisher","first-page":"373","DOI":"10.1016\/0005-2728(94)90058-2","volume":"1188","author":"Z Khuchua","year":"1994","unstructured":"Khuchua, Z. et al. Caffeine and Ca2+ stimulate mitochondrial oxidative phosphorylation in saponin-skinned human skeletal muscle fibers due to activation of actomyosin ATPase. Biochim. Biophys. Acta 1188, 373\u2013379 (1994).","journal-title":"Biochim. Biophys. Acta"},{"key":"BFnprot200861_CR32","doi-asserted-by":"publisher","first-page":"188","DOI":"10.1016\/0014-5793(93)81477-H","volume":"323","author":"WS Kunz","year":"1993","unstructured":"Kunz, W.S., Kuznetsov, A.V. & Gellerich, F.N. Mitochondrial oxidative phosphorylation in saponin-skinned human muscle fibers is stimulated by caffeine. FEBS Lett. 323, 188\u2013190 (1993).","journal-title":"FEBS Lett."},{"key":"BFnprot200861_CR33","doi-asserted-by":"publisher","first-page":"142","DOI":"10.1016\/S0925-4439(96)00072-5","volume":"1360","author":"AV Kuznetsov","year":"1997","unstructured":"Kuznetsov, A.V. et al. Application of inhibitor titrations for the detection of oxidative phosphorylation defects in saponin-skinned muscle fibers of patients with mitochondrial diseases. Biochim. Biophys. Acta 1360, 142\u2013150 (1997).","journal-title":"Biochim. Biophys. Acta"},{"key":"BFnprot200861_CR34","doi-asserted-by":"publisher","first-page":"221","DOI":"10.1002\/biof.5520070312","volume":"7","author":"AV Kuznetsov","year":"1998","unstructured":"Kuznetsov, A.V., Wiedemann, F.R., Winkler, K. & Kunz, W.S. Use of saponin-permeabilized muscle fibers for the diagnosis of mitochondrial diseases. Biofactors 7, 221\u2013223 (1998).","journal-title":"Biofactors"},{"key":"BFnprot200861_CR35","first-page":"H1633","volume":"286","author":"AV Kuznetsov","year":"2004","unstructured":"Kuznetsov, A.V. et al. Mitochondrial defects and heterogeneous cytochrome c release after cardiac cold ischemia and reperfusion. Am. J. Physiol. 286, H1633\u2013H1641 (2004).","journal-title":"Am. J. Physiol."},{"key":"BFnprot200861_CR36","doi-asserted-by":"crossref","first-page":"14325","DOI":"10.1016\/S0021-9258(17)38721-5","volume":"260","author":"RA Altschuld","year":"1985","unstructured":"Altschuld, R.A. et al. Structural and functional properties of adult rat heart myocytes lysed with digitonin. J. Biol. Chem. 260, 14325\u201314334 (1985).","journal-title":"J. Biol. Chem."},{"key":"BFnprot200861_CR37","doi-asserted-by":"publisher","first-page":"10156","DOI":"10.1073\/pnas.152259999","volume":"99","author":"PP Dzeja","year":"2002","unstructured":"Dzeja, P.P., Bortolon, R., Perez-Terzic, C., Holmuhamedov, E.L. & Terzic, A. Energetic communication between mitochondria and nucleus directed by catalyzed phosphotransfer. Proc. Natl. Acad. Sci. USA 99, 10156\u201310161 (2002).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnprot200861_CR38","doi-asserted-by":"publisher","first-page":"1763","DOI":"10.1126\/science.280.5370.1763","volume":"280","author":"R Rizzuto","year":"1998","unstructured":"Rizzuto, R. et al. Close contacts with the endoplasmic reticulum as determinants of mitochondrial Ca2+ responses. Science 280, 1763\u20131766 (1998).","journal-title":"Science"},{"key":"BFnprot200861_CR39","doi-asserted-by":"publisher","first-page":"915","DOI":"10.1083\/jcb.200604016","volume":"174","author":"G Csord\u00e1s","year":"2006","unstructured":"Csord\u00e1s, G. et al. Structural and functional features and significance of the physical linkage between ER and mitochondria. J. Cell Biol. 174, 915\u2013921 (2006).","journal-title":"J. Cell Biol."},{"key":"BFnprot200861_CR40","doi-asserted-by":"publisher","first-page":"776","DOI":"10.1126\/science.1138870","volume":"315","author":"RJ Youle","year":"2007","unstructured":"Youle, R.J. et al. Cellular demolition and the rules. Science 315, 776 (2007).","journal-title":"Science"},{"key":"BFnprot200861_CR41","doi-asserted-by":"publisher","first-page":"405","DOI":"10.1038\/nrm2153","volume":"8","author":"SJ Riedl","year":"2007","unstructured":"Riedl, S.J. & Salvesen, G.S. The apoptosome: signalling platform of cell death. Nat. Rev. Mol. Cell. Biol. 8, 405\u2013413 (2007).","journal-title":"Nat. Rev. Mol. Cell. Biol."},{"key":"BFnprot200861_CR42","doi-asserted-by":"publisher","first-page":"658","DOI":"10.1038\/nature05111","volume":"443","author":"M Karbowski","year":"2006","unstructured":"Karbowski, M., Norris, K.L., Cleland, M.M., Jeong, S.Y. & Youle, R.J. Role of Bax and Bak in mitochondrial morphogenesis. Nature 443, 658\u2013662 (2006).","journal-title":"Nature"},{"key":"BFnprot200861_CR43","doi-asserted-by":"publisher","first-page":"302","DOI":"10.1016\/0304-4165(91)90168-G","volume":"1074","author":"VA Saks","year":"1991","unstructured":"Saks, V.A., Belikova, Y.O. & Kuznetsov, A.V. In vivo regulation of mitochondrial respiration in cardiomyocytes: specific restrictions for intracellular diffusion of ADP. Biochim. Biophys. Acta 1074, 302\u2013311 (1991).","journal-title":"Biochim. Biophys. Acta"},{"key":"BFnprot200861_CR44","doi-asserted-by":"publisher","first-page":"134","DOI":"10.1016\/0005-2728(93)90166-D","volume":"1144","author":"VA Saks","year":"1993","unstructured":"Saks, V.A. et al. Retarded diffusion of ADP in cardiomyocytes: possible role of mitochondrial outer membrane and creatine kinase in cellular regulation of oxidative phosphorylation. Biochim. Biophys. Acta 1144, 134\u2013148 (1993).","journal-title":"Biochim. Biophys. Acta"},{"key":"BFnprot200861_CR45","doi-asserted-by":"publisher","first-page":"909","DOI":"10.1111\/j.1432-1033.1996.00909.x","volume":"241","author":"AV Kuznetsov","year":"1996","unstructured":"Kuznetsov, A.V. et al. Striking differences between the kinetics of regulation of respiration by ADP in slow-twitch and fast-twitch muscles in vivo. Eur. J. Biochem. 241, 909\u2013915 (1996).","journal-title":"Eur. J. Biochem."},{"key":"BFnprot200861_CR46","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1161\/01.RES.69.3.665","volume":"69","author":"JA Hoerter","year":"1991","unstructured":"Hoerter, J.A., Kuznetsov, A.V. & Ventura-Clapier, R. Functional development of creatine kinase system in perinatal rabbit heart. Circ. Res. 69, 665\u2013676 (1991).","journal-title":"Circ. Res."},{"key":"BFnprot200861_CR47","doi-asserted-by":"publisher","first-page":"1422","DOI":"10.1046\/j.1432-1327.2001.02012.x","volume":"268","author":"S Trumbeckaite","year":"2001","unstructured":"Trumbeckaite, S., Opalka, J.R., Neuhof, C., Zierz, S. & Gellerich, F.N. Different sensitivity of rabbit heart and skeletal muscle to endotoxin-induced impairment of mitochondrial function. Eur. J. Biochem. 268, 1422\u20131429 (2001).","journal-title":"Eur. J. Biochem."},{"key":"BFnprot200861_CR48","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1074\/jbc.271.1.283","volume":"271","author":"AV Kuznetsov","year":"1996","unstructured":"Kuznetsov, A.V., Clark, J.F., Winkler, K. & Kunz, W.S. Increase of flux control of cytochrome c oxidase in copper-deficient mottled brindled mice. J. Biol. Chem. 271, 283\u2013288 (1996).","journal-title":"J. Biol. Chem."},{"key":"BFnprot200861_CR49","doi-asserted-by":"publisher","first-page":"19921","DOI":"10.1074\/jbc.270.34.19921","volume":"270","author":"VI Veksler","year":"1995","unstructured":"Veksler, V.I. et al. Muscle creatine kinase deficient mice: cardiac and skeletal muscle tissue-specificity of adaptation of the mitochondrial function. J. Biol. Chem. 270, 19921\u201319929 (1995).","journal-title":"J. Biol. Chem."},{"key":"BFnprot200861_CR50","doi-asserted-by":"publisher","first-page":"14785","DOI":"10.1074\/jbc.271.25.14785","volume":"271","author":"P Rustin","year":"1996","unstructured":"Rustin, P. et al. Fluxes of nicotinamide adenine dinucleotides through mitochondrial membranes in human cultured cells. J. Biol. Chem. 271, 14785\u201314790 (1996).","journal-title":"J. Biol. Chem."},{"key":"BFnprot200861_CR51","doi-asserted-by":"publisher","first-page":"431","DOI":"10.1007\/978-3-662-04162-8_45","volume-title":"Life in the Cold","author":"E Gnaiger","year":"2000","unstructured":"Gnaiger, E. et al. Mitochondria in the cold. In Life in the Cold (eds. G. Heldmaier & M. Klingenspor) Springer, Berlin, Heidelberg, New York, 431\u2013442 (2000)."},{"key":"BFnprot200861_CR52","doi-asserted-by":"publisher","first-page":"296","DOI":"10.1016\/S0003-2697(03)00326-9","volume":"319","author":"AV Kuznetsov","year":"2003","unstructured":"Kuznetsov, A.V. et al. Cryopreservation of mitochondria and mitochondrial function in cardiac and skeletal muscle fibers. Anal. Biochem. 319, 296\u2013303 (2003).","journal-title":"Anal. Biochem."},{"key":"BFnprot200861_CR53","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1113\/jphysiol.2003.055095","volume":"555","author":"R Ventura-Clapier","year":"2004","unstructured":"Ventura-Clapier, R., Garnier, A. & Veksler, V. Energy metabolism in heart failure. J. Physiol. 555, 1\u201313 (2004).","journal-title":"J. Physiol."},{"key":"BFnprot200861_CR54","first-page":"223","volume":"4","author":"ZA Khuchua","year":"1992","unstructured":"Khuchua, Z.A. et al. The creatine kinase system and cardiomyopathy. Am. J. Cardiovasc. Pathol. 4, 223\u2013234 (1992).","journal-title":"Am. J. Cardiovasc. Pathol."},{"key":"BFnprot200861_CR55","doi-asserted-by":"publisher","first-page":"68","DOI":"10.1161\/01.RES.85.1.68","volume":"85","author":"E De Sousa","year":"1999","unstructured":"De Sousa, E. et al. Subcellular creatine kinase alterations. Implications in heart failure. Circ. Res. 85, 68\u201376 (1999).","journal-title":"Circ. Res."},{"key":"BFnprot200861_CR56","doi-asserted-by":"publisher","first-page":"75","DOI":"10.1016\/S0014-5793(99)00033-2","volume":"444","author":"A Kaasik","year":"1999","unstructured":"Kaasik, A. et al. Nitric oxide inhibits cardiac energy production via inhibition of mitochondrial creatine kinase. FEBS Lett. 444, 75\u201377 (1999).","journal-title":"FEBS Lett."}],"container-title":["Nature Protocols"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nprot.2008.61.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nprot.2008.61","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nprot.2008.61.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,18]],"date-time":"2023-05-18T20:10:16Z","timestamp":1684440616000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nprot.2008.61"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,5,15]]},"references-count":56,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2008,6]]}},"alternative-id":["BFnprot200861"],"URL":"https:\/\/doi.org\/10.1038\/nprot.2008.61","relation":{},"ISSN":["1754-2189","1750-2799"],"issn-type":[{"value":"1754-2189","type":"print"},{"value":"1750-2799","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,5,15]]}}}