{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T22:57:39Z","timestamp":1791154659462,"version":"4.1.0"},"reference-count":35,"publisher":"Springer Science and Business Media LLC","issue":"12","license":[{"start":{"date-parts":[[2003,11,9]],"date-time":"2003-11-09T00:00:00Z","timestamp":1068336000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nat Struct Mol Biol"],"published-print":{"date-parts":[[2003,12]]},"DOI":"10.1038\/nsb1011","type":"journal-article","created":{"date-parts":[[2003,11,9]],"date-time":"2003-11-09T19:19:03Z","timestamp":1068405543000},"page":"995-1001","source":"Crossref","is-referenced-by-count":149,"title":["Bistable regulation of integrin adhesiveness by a bipolar metal ion cluster"],"prefix":"10.1038","volume":"10","author":[{"given":"JianFeng","family":"Chen","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Azucena","family":"Salas","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy A","family":"Springer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2003,11,9]]},"reference":[{"key":"BFnsb1011_CR1","doi-asserted-by":"publisher","first-page":"485","DOI":"10.1146\/annurev.biophys.31.101101.140922","volume":"31","author":"M Shimaoka","year":"2002","unstructured":"Shimaoka, M., Takagi, J. & Springer, T.A. Conformational regulation of integrin structure and function. Annu. Rev. Biophys. Biomol. Struct. 31, 485\u2013516 (2002).","journal-title":"Annu. Rev. Biophys. Biomol. Struct."},{"key":"BFnsb1011_CR2","doi-asserted-by":"publisher","first-page":"47","DOI":"10.1016\/S0092-8674(00)80622-4","volume":"101","author":"J Emsley","year":"2000","unstructured":"Emsley, J., Knight, C.G., Farndale, R.W., Barnes, M.J. & Liddington, R.C. Structural basis of collagen recognition by integrin \u03b12\u03b21. Cell 101, 47\u201356 (2000).","journal-title":"Cell"},{"key":"BFnsb1011_CR3","doi-asserted-by":"publisher","first-page":"282","DOI":"10.1038\/nsb779","volume":"9","author":"N Beglova","year":"2002","unstructured":"Beglova, N., Blacklow, S.C., Takagi, J. & Springer, T.A. Cysteine-rich module structure reveals a fulcrum for integrin rearrangement upon activation. Nat. Struct. Biol. 9, 282\u2013287 (2002).","journal-title":"Nat. Struct. Biol."},{"key":"BFnsb1011_CR4","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/S0092-8674(02)01257-6","volume":"112","author":"M Shimaoka","year":"2003","unstructured":"Shimaoka, M. et al. Structures of the \u03b1L I domain and its complex with ICAM-1 reveal a shape-shifting pathway for integrin regulation. Cell 112, 99\u2013111 (2003).","journal-title":"Cell"},{"key":"BFnsb1011_CR5","doi-asserted-by":"publisher","first-page":"599","DOI":"10.1016\/S0092-8674(02)00935-2","volume":"110","author":"J Takagi","year":"2002","unstructured":"Takagi, J., Petre, B.M., Walz, T. & Springer, T.A. Global conformational rearrangements in integrin extracellular domains in outside-in and inside-out signaling. Cell 110, 599\u2013611 (2002).","journal-title":"Cell"},{"key":"BFnsb1011_CR6","doi-asserted-by":"publisher","first-page":"4607","DOI":"10.1093\/emboj\/cdg445","volume":"22","author":"J Takagi","year":"2003","unstructured":"Takagi, J., Strokovich, K., Springer, T.A. & Walz, T. Structure of integrin \u03b15\u03b21 in complex with fibronectin. EMBO J. 22, 4607\u20134615 (2003).","journal-title":"EMBO J."},{"key":"BFnsb1011_CR7","doi-asserted-by":"publisher","first-page":"339","DOI":"10.1126\/science.1064535","volume":"294","author":"J-P Xiong","year":"2001","unstructured":"Xiong, J.-P. et al. Crystal structure of the extracellular segment of integrin \u03b1V\u03b23. Science 294, 339\u2013345 (2001).","journal-title":"Science"},{"key":"BFnsb1011_CR8","doi-asserted-by":"publisher","first-page":"151","DOI":"10.1126\/science.1069040","volume":"296","author":"JP Xiong","year":"2002","unstructured":"Xiong, J.P. et al. Crystal structure of the extracellular segment of integrin \u03b1V\u03b23 in complex with an Arg-Gly-Asp ligand. Science 296, 151\u2013155 (2002).","journal-title":"Science"},{"key":"BFnsb1011_CR9","doi-asserted-by":"publisher","first-page":"813","DOI":"10.1016\/0092-8674(87)90104-8","volume":"51","author":"SD Marlin","year":"1987","unstructured":"Marlin, S.D. & Springer, T.A. Purified intercellular adhesion molecule-1 (ICAM-1) is a ligand for lymphocyte function-associated antigen 1 (LFA-1). Cell 51, 813\u2013819 (1987).","journal-title":"Cell"},{"key":"BFnsb1011_CR10","doi-asserted-by":"crossref","first-page":"12927","DOI":"10.1016\/S0021-9258(18)37650-6","volume":"263","author":"J Gailit","year":"1988","unstructured":"Gailit, J. & Ruoslahti, E. Regulation of the fibronectin receptor affinity by divalent cations. J. Biol. Chem. 263, 12927\u201312932 (1988).","journal-title":"J. Biol. Chem."},{"key":"BFnsb1011_CR11","doi-asserted-by":"publisher","first-page":"219","DOI":"10.1083\/jcb.116.1.219","volume":"116","author":"I Dransfield","year":"1992","unstructured":"Dransfield, I., Caba\u00f1as, C., Craig, A. & Hogg, N. Divalent cation regulation of the function of the leukocyte integrin LFA-1. J. Cell Biol. 116, 219\u2013226 (1992).","journal-title":"J. Cell Biol."},{"key":"BFnsb1011_CR12","doi-asserted-by":"publisher","first-page":"1917","DOI":"10.1083\/jcb.108.5.1917","volume":"108","author":"WD Staatz","year":"1989","unstructured":"Staatz, W.D., Rajpara, S.M., Wayner, E.A., Carter, W.G. & Santoro, S.A. The membrane glycoprotein Ia-IIa (VLA-2) complex mediates the Mg++-dependent adhesion of platelets to collagen. J. Cell Biol. 108, 1917\u20131924 (1989).","journal-title":"J. Cell Biol."},{"key":"BFnsb1011_CR13","doi-asserted-by":"publisher","first-page":"26270","DOI":"10.1074\/jbc.270.44.26270","volume":"270","author":"AP Mould","year":"1995","unstructured":"Mould, A.P., Akiyama, S.K. & Humphries, M.J. Regulation of integrin \u03b15\u03b21-fibronectin interactions by divalent cations. J. Biol. Chem. 270, 26270\u201326277 (1995).","journal-title":"J. Biol. Chem."},{"key":"BFnsb1011_CR14","doi-asserted-by":"publisher","first-page":"9917","DOI":"10.1074\/jbc.270.17.9917","volume":"270","author":"DD Hu","year":"1995","unstructured":"Hu, D.D., Hoyer, J.R. & Smith, J.W. Ca2+ suppresses cell adhesion to osteopontin by attenuating binding affinity for integrin \u03b1v\u03b23. J. Biol. Chem. 270, 9917\u20139925 (1995).","journal-title":"J. Biol. Chem."},{"key":"BFnsb1011_CR15","doi-asserted-by":"publisher","first-page":"91","DOI":"10.1016\/S0167-4889(00)00086-0","volume":"1498","author":"B Leitinger","year":"2000","unstructured":"Leitinger, B., McDowall, A., Stanley, P. & Hogg, N. The regulation of integrin function by Ca2+. Biochim. Biophys. Acta 1498, 91\u201398 (2000).","journal-title":"Biochim. Biophys. Acta"},{"key":"BFnsb1011_CR16","doi-asserted-by":"publisher","first-page":"413","DOI":"10.1016\/0092-8674(95)90491-3","volume":"80","author":"C Berlin","year":"1995","unstructured":"Berlin, C. et al. \u03b14 integrins mediate lymphocyte attachment and rolling under physiologic flow. Cell 80, 413\u2013422 (1995).","journal-title":"Cell"},{"key":"BFnsb1011_CR17","doi-asserted-by":"publisher","first-page":"1243","DOI":"10.1083\/jcb.128.6.1243","volume":"128","author":"R Alon","year":"1995","unstructured":"Alon, R. et al. The integrin VLA-4 supports tethering and rolling in flow on VCAM-1. J. Cell Biol. 128, 1243\u20131253 (1995).","journal-title":"J. Cell Biol."},{"key":"BFnsb1011_CR18","doi-asserted-by":"publisher","first-page":"13972","DOI":"10.1021\/bi011582f","volume":"40","author":"M de Chateau","year":"2001","unstructured":"de Chateau, M., Chen, S., Salas, A. & Springer, T.A. Kinetic and mechanical basis of rolling through an integrin and novel Ca2+-dependent rolling and Mg2+-dependent firm adhesion modalities for the \u03b14\u03b27\u2013MAdCAM-1 interaction. Biochemistry 40, 13972\u201313979 (2001).","journal-title":"Biochemistry"},{"key":"BFnsb1011_CR19","doi-asserted-by":"publisher","first-page":"802","DOI":"10.1016\/S0959-440X(02)00384-6","volume":"12","author":"TA Springer","year":"2002","unstructured":"Springer, T.A. Predicted and experimental structures of integrins and \u03b2-propellers. Curr. Opin. Struct. Biol. 12, 802\u2013813 (2002).","journal-title":"Curr. Opin. Struct. Biol."},{"key":"BFnsb1011_CR20","doi-asserted-by":"publisher","first-page":"2647","DOI":"10.1091\/mbc.8.12.2647","volume":"8","author":"C Pujades","year":"1997","unstructured":"Pujades, C. et al. Defining extracellular integrin \u03b1 chain sites that affect cell adhesion and adhesion strengthening without altering soluble ligand binding. Mol. Biol. Cell 8, 2647\u20132657 (1997).","journal-title":"Mol. Biol. Cell"},{"key":"BFnsb1011_CR21","doi-asserted-by":"publisher","first-page":"719","DOI":"10.1084\/jem.186.5.719","volume":"186","author":"R Knorr","year":"1997","unstructured":"Knorr, R. & Dustin, M.L. The lymphocyte function-associated antigen 1 I domain is a transient binding module for intercellular adhesion molecule (ICAM)-1 and ICAM-1 in hydrodynamic flow. J. Exp. Med. 186, 719\u2013730 (1997).","journal-title":"J. Exp. Med."},{"key":"BFnsb1011_CR22","doi-asserted-by":"publisher","first-page":"2393","DOI":"10.1073\/pnas.041618598","volume":"98","author":"C Lu","year":"2001","unstructured":"Lu, C., Shimaoka, M., Zang, Q., Takagi, J. & Springer, T.A. Locking in alternate conformations of the integrin \u03b1L\u03b22 I domain with disulfide bonds reveals functional relationships among integrin domains. Proc. Natl. Acad. Sci. USA 98, 2393\u20132398 (2001).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnsb1011_CR23","doi-asserted-by":"publisher","first-page":"50255","DOI":"10.1074\/jbc.M209822200","volume":"277","author":"A Salas","year":"2002","unstructured":"Salas, A., Shimaoka, M., Chen, S., Carman, C.V. & Springer, T.A. Transition from rolling to firm adhesion is regulated by the conformation of the I domain of the integrin LFA-1. J. Biol. Chem. 277, 50255\u201350262 (2002).","journal-title":"J. Biol. Chem."},{"key":"BFnsb1011_CR24","doi-asserted-by":"crossref","first-page":"185","DOI":"10.1016\/0092-8674(93)90305-A","volume":"74","author":"C Berlin","year":"1993","unstructured":"Berlin, C. et al. \u03b14\u03b27 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74, 185\u2013195 (1993).","journal-title":"Cell"},{"key":"BFnsb1011_CR25","doi-asserted-by":"publisher","first-page":"99","DOI":"10.1016\/1074-7613(95)90162-0","volume":"3","author":"RF Bargatze","year":"1995","unstructured":"Bargatze, R.F., Jutila, M.A. & Butcher, E.C. Distinct roles of L-selectin and integrins \u03b14\u03b27 and LFA-1 in lymphocyte homing to Peyer's patch-HEV in situ: the multistep model confirmed and refined. Immunity 3, 99\u2013108 (1995).","journal-title":"Immunity"},{"key":"BFnsb1011_CR26","doi-asserted-by":"publisher","first-page":"461","DOI":"10.1038\/363461a0","volume":"363","author":"MJ Briskin","year":"1993","unstructured":"Briskin, M.J., McEvoy, L.M. & Butcher, E.C. MAdCAM-1 has homology to immunoglobulin and mucin-like adhesion receptors and to IgA1. Nature 363, 461\u2013464 (1993).","journal-title":"Nature"},{"key":"BFnsb1011_CR27","doi-asserted-by":"publisher","first-page":"11262","DOI":"10.1073\/pnas.200240897","volume":"97","author":"K-C Chang","year":"2000","unstructured":"Chang, K.-C., Tees, D.F. & Hammer, D.A. The state diagram for cell adhesion under flow: leukocyte rolling and firm adhesion. Proc. Natl. Acad. Sci. USA 97, 11262\u201311267 (2000).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnsb1011_CR28","doi-asserted-by":"publisher","first-page":"401","DOI":"10.1107\/S0907444900019168","volume":"57","author":"MM Harding","year":"2001","unstructured":"Harding, M.M. Geometry of metal-ligand interactions in proteins. Acta Crystallogr. D 57, 401\u2013411 (2001).","journal-title":"Acta Crystallogr. D"},{"key":"BFnsb1011_CR29","doi-asserted-by":"crossref","first-page":"1497","DOI":"10.4049\/jimmunol.159.3.1497","volume":"159","author":"M Tidswell","year":"1997","unstructured":"Tidswell, M. et al. Structure-function analysis of the integrin \u03b27 subunit: Identification of domains involved in adhesion to MAdCAM-1. J. Immunol. 159, 1497\u20131505 (1997).","journal-title":"J. Immunol."},{"key":"BFnsb1011_CR30","doi-asserted-by":"crossref","first-page":"268","DOI":"10.4049\/jimmunol.159.1.268","volume":"159","author":"C Lu","year":"1997","unstructured":"Lu, C. & Springer, T.A. The \u03b1 subunit cytoplasmic domain regulates the assembly and adhesiveness of integrin lymphocyte function-associated antigen-1 (LFA-1). J. Immunol. 159, 268\u2013278 (1997).","journal-title":"J. Immunol."},{"key":"BFnsb1011_CR31","doi-asserted-by":"publisher","first-page":"15138","DOI":"10.1074\/jbc.273.24.15138","volume":"273","author":"C Lu","year":"1998","unstructured":"Lu, C., Oxvig, C. & Springer, T.A. The structure of the \u03b2-propeller domain and C-terminal region of the integrin \u03b1M subunit. J. Biol. Chem. 273, 15138\u201315147 (1998).","journal-title":"J. Biol. Chem."},{"key":"BFnsb1011_CR32","doi-asserted-by":"publisher","first-page":"649","DOI":"10.1084\/jem.178.2.649","volume":"178","author":"PD Kassner","year":"1993","unstructured":"Kassner, P.D. & Hemler, M.E. Interchangeable \u03b1 chain cytoplasmic domains play a positive role in control of cell adhesion mediated by VLA-4, a \u03b21 integrin. J. Exp. Med. 178, 649\u2013660 (1993).","journal-title":"J. Exp. Med."},{"key":"BFnsb1011_CR33","doi-asserted-by":"crossref","first-page":"1857","DOI":"10.4049\/jimmunol.133.4.1857","volume":"133","author":"AI Lazarovits","year":"1984","unstructured":"Lazarovits, A.I. et al. Lymphocyte activation antigens: I. A monoclonal antibody, anti-act I, defines a new late lymphocyte activation antigen. J. Immunol. 133, 1857\u20131862 (1984).","journal-title":"J. Immunol."},{"key":"BFnsb1011_CR34","doi-asserted-by":"crossref","first-page":"717","DOI":"10.4049\/jimmunol.151.2.717","volume":"151","author":"T Schweighoffer","year":"1993","unstructured":"Schweighoffer, T. et al. Selective expression of integrin \u03b14\u03b27 on a subset of human CD4+ memory T cells with hallmarks of gut-trophism. J. Immunol. 151, 717\u2013729 (1993).","journal-title":"J. Immunol."},{"key":"BFnsb1011_CR35","doi-asserted-by":"publisher","first-page":"859","DOI":"10.1016\/0092-8674(91)90393-D","volume":"65","author":"MB Lawrence","year":"1991","unstructured":"Lawrence, M.B. & Springer, T.A. Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins. Cell 65, 859\u2013873 (1991).","journal-title":"Cell"}],"container-title":["Nature Structural & Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nsb1011.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nsb1011","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nsb1011.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T04:21:13Z","timestamp":1684470073000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nsb1011"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,11,9]]},"references-count":35,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2003,12]]}},"alternative-id":["BFnsb1011"],"URL":"https:\/\/doi.org\/10.1038\/nsb1011","relation":{},"ISSN":["1545-9993","1545-9985"],"issn-type":[{"value":"1545-9993","type":"print"},{"value":"1545-9985","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,11,9]]}}}