{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T07:36:10Z","timestamp":1789803370085,"version":"4.0.1"},"reference-count":45,"publisher":"Springer Science and Business Media LLC","issue":"10","license":[{"start":{"date-parts":[[2009,9,27]],"date-time":"2009-09-27T00:00:00Z","timestamp":1254009600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Nat Struct Mol Biol"],"published-print":{"date-parts":[[2009,10]]},"DOI":"10.1038\/nsmb.1679","type":"journal-article","created":{"date-parts":[[2009,9,27]],"date-time":"2009-09-27T17:52:02Z","timestamp":1254073922000},"page":"1080-1085","source":"Crossref","is-referenced-by-count":136,"title":["Interactions between lipids and voltage sensor paddles detected with tarantula toxins"],"prefix":"10.1038","volume":"16","author":[{"given":"Mirela","family":"Milescu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Bosmans","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Seungkyu","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"AbdulRasheed A","family":"Alabi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jae Il","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenton J","family":"Swartz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2009,9,27]]},"reference":[{"key":"BFnsmb1679_CR1","doi-asserted-by":"publisher","first-page":"376","DOI":"10.1038\/nature06265","volume":"450","author":"SB Long","year":"2007","unstructured":"Long, S.B., Tao, X., Campbell, E.B. & MacKinnon, R. Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment. Nature 450, 376\u2013382 (2007).","journal-title":"Nature"},{"key":"BFnsmb1679_CR2","doi-asserted-by":"publisher","first-page":"69","DOI":"10.1126\/science.280.5360.69","volume":"280","author":"DA Doyle","year":"1998","unstructured":"Doyle, D.A. et al. The structure of the potassium channel: molecular basis of K+ conduction and selectivity. Science 280, 69\u201377 (1998).","journal-title":"Science"},{"key":"BFnsmb1679_CR3","doi-asserted-by":"publisher","first-page":"891","DOI":"10.1038\/nature07620","volume":"456","author":"KJ Swartz","year":"2008","unstructured":"Swartz, K.J. Sensing voltage across lipid membranes. Nature 456, 891\u2013897 (2008).","journal-title":"Nature"},{"key":"BFnsmb1679_CR4","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1038\/nature01580","volume":"423","author":"Y Jiang","year":"2003","unstructured":"Jiang, Y. et al. X-ray structure of a voltage-dependent K+ channel. Nature 423, 33\u201341 (2003).","journal-title":"Nature"},{"key":"BFnsmb1679_CR5","doi-asserted-by":"publisher","first-page":"775","DOI":"10.1038\/nature05416","volume":"444","author":"D Schmidt","year":"2006","unstructured":"Schmidt, D., Jiang, Q.X. & MacKinnon, R. Phospholipids and the origin of cationic gating charges in voltage sensors. Nature 444, 775\u2013779 (2006).","journal-title":"Nature"},{"key":"BFnsmb1679_CR6","doi-asserted-by":"publisher","first-page":"696","DOI":"10.1038\/nature04880","volume":"442","author":"Y Ramu","year":"2006","unstructured":"Ramu, Y., Xu, Y. & Lu, Z. Enzymatic activation of voltage-gated potassium channels. Nature 442, 696\u2013699 (2006).","journal-title":"Nature"},{"key":"BFnsmb1679_CR7","doi-asserted-by":"publisher","first-page":"826","DOI":"10.1038\/nature06618","volume":"451","author":"Y Xu","year":"2008","unstructured":"Xu, Y., Ramu, Y. & Lu, Z. Removal of phospho-head groups of membrane lipids immobilizes voltage sensors of K+ channels. Nature 451, 826\u2013829 (2008).","journal-title":"Nature"},{"key":"BFnsmb1679_CR8","doi-asserted-by":"publisher","first-page":"370","DOI":"10.1038\/nature06266","volume":"450","author":"AA Alabi","year":"2007","unstructured":"Alabi, A.A., Bahamonde, M.I., Jung, H.J., Kim, J.I. & Swartz, K.J. Portability of paddle motif function and pharmacology in voltage sensors. Nature 450, 370\u2013375 (2007).","journal-title":"Nature"},{"key":"BFnsmb1679_CR9","doi-asserted-by":"publisher","first-page":"497","DOI":"10.1085\/jgp.200709869","volume":"130","author":"M Milescu","year":"2007","unstructured":"Milescu, M. et al. Tarantula toxins interact with voltage sensors within lipid membranes. J. Gen. Physiol. 130, 497\u2013511 (2007).","journal-title":"J. Gen. Physiol."},{"key":"BFnsmb1679_CR10","doi-asserted-by":"publisher","first-page":"19276","DOI":"10.1073\/pnas.0810187105","volume":"105","author":"D Schmidt","year":"2008","unstructured":"Schmidt, D. & Mackinnon, R. Voltage-dependent K+ channel gating and voltage sensor toxin sensitivity depend on the mechanical state of the lipid membrane. Proc. Natl. Acad. Sci. USA 105, 19276\u201319281 (2008).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnsmb1679_CR11","doi-asserted-by":"publisher","first-page":"421","DOI":"10.1016\/0896-6273(88)90192-4","volume":"1","author":"A Kamb","year":"1988","unstructured":"Kamb, A., Tseng-Crank, J. & Tanouye, M.A. Multiple products of the Drosophila Shaker gene may contribute to potassium channel diversity. Neuron 1, 421\u2013430 (1988).","journal-title":"Neuron"},{"key":"BFnsmb1679_CR12","doi-asserted-by":"publisher","first-page":"642","DOI":"10.1038\/340642a0","volume":"340","author":"GC Frech","year":"1989","unstructured":"Frech, G.C., VanDongen, A.M., Schuster, G., Brown, A.M. & Joho, R.H. A novel potassium channel with delayed rectifier properties isolated from rat brain by expression cloning. Nature 340, 642\u2013645 (1989).","journal-title":"Nature"},{"key":"BFnsmb1679_CR13","doi-asserted-by":"publisher","first-page":"2","DOI":"10.1016\/j.bbamem.2004.06.012","volume":"1666","author":"H Palsdottir","year":"2004","unstructured":"Palsdottir, H. & Hunte, C. Lipids in membrane protein structures. Biochim. Biophys. Acta 1666, 2\u201318 (2004).","journal-title":"Biochim. Biophys. Acta"},{"key":"BFnsmb1679_CR14","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1016\/S0005-2736(03)00056-7","volume":"1612","author":"AG Lee","year":"2003","unstructured":"Lee, A.G. Lipid-protein interactions in biological membranes: a structural perspective. Biochim. Biophys. Acta 1612, 1\u201340 (2003).","journal-title":"Biochim. Biophys. Acta"},{"key":"BFnsmb1679_CR15","doi-asserted-by":"publisher","first-page":"1239","DOI":"10.1038\/nature03650","volume":"435","author":"Y Murata","year":"2005","unstructured":"Murata, Y., Iwasaki, H., Sasaki, M., Inaba, K. & Okamura, Y. Phosphoinositide phosphatase activity coupled to an intrinsic voltage sensor. Nature 435, 1239\u20131243 (2005).","journal-title":"Nature"},{"key":"BFnsmb1679_CR16","doi-asserted-by":"publisher","first-page":"17600","DOI":"10.1073\/pnas.0807387105","volume":"105","author":"CA Villalba-Galea","year":"2008","unstructured":"Villalba-Galea, C.A., Sandtner, W., Starace, D.M. & Bezanilla, F. S4-based voltage sensors have three major conformations. Proc. Natl. Acad. Sci. USA 105, 17600\u201317607 (2008).","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"BFnsmb1679_CR17","doi-asserted-by":"publisher","first-page":"5","DOI":"10.1085\/jgp.200910215","volume":"134","author":"CA Villalba-Galea","year":"2009","unstructured":"Villalba-Galea, C.A., Miceli, F., Taglialatela, M. & Bezanilla, F. Coupling between the voltage-sensing and phosphatase domains of Ci-VSP. J. Gen. Physiol. 134, 5\u201314 (2009).","journal-title":"J. Gen. Physiol."},{"key":"BFnsmb1679_CR18","doi-asserted-by":"publisher","first-page":"491","DOI":"10.1126\/science.1101373","volume":"306","author":"LG Cuello","year":"2004","unstructured":"Cuello, L.G., Cortes, D.M. & Perozo, E. Molecular architecture of the KvAP voltage-dependent K+ channel in a lipid bilayer. Science 306, 491\u2013495 (2004).","journal-title":"Science"},{"key":"BFnsmb1679_CR19","doi-asserted-by":"publisher","first-page":"463","DOI":"10.1016\/j.cell.2005.08.041","volume":"123","author":"V Ruta","year":"2005","unstructured":"Ruta, V., Chen, J. & MacKinnon, R. Calibrated measurement of gating-charge arginine displacement in the KvAP voltage-dependent K+ channel. Cell 123, 463\u2013475 (2005).","journal-title":"Cell"},{"key":"BFnsmb1679_CR20","doi-asserted-by":"publisher","first-page":"569","DOI":"10.1016\/j.jmb.2008.06.010","volume":"381","author":"A Banerjee","year":"2008","unstructured":"Banerjee, A. & MacKinnon, R. Inferred motions of the S3a helix during voltage-dependent K+ channel gating. J. Mol. Biol. 381, 569\u2013580 (2008).","journal-title":"J. Mol. Biol."},{"key":"BFnsmb1679_CR21","doi-asserted-by":"publisher","first-page":"202","DOI":"10.1038\/nature07473","volume":"456","author":"F Bosmans","year":"2008","unstructured":"Bosmans, F., Martin-Eauclaire, M.F. & Swartz, K.J. Deconstructing voltage sensor function and pharmacology in sodium channels. Nature 456, 202\u2013208 (2008).","journal-title":"Nature"},{"key":"BFnsmb1679_CR22","doi-asserted-by":"publisher","first-page":"398","DOI":"10.1016\/j.str.2007.12.015","volume":"16","author":"S Chakrapani","year":"2008","unstructured":"Chakrapani, S., Cuello, L.G., Cortes, D.M. & Perozo, E. Structural dynamics of an isolated voltage-sensor domain in a lipid bilayer. Structure 16, 398\u2013409 (2008).","journal-title":"Structure"},{"key":"BFnsmb1679_CR23","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1016\/0896-6273(89)90113-X","volume":"3","author":"JS Trimmer","year":"1989","unstructured":"Trimmer, J.S. et al. Primary structure and functional expression of a mammalian skeletal muscle sodium channel. Neuron 3, 33\u201349 (1989).","journal-title":"Neuron"},{"key":"BFnsmb1679_CR24","doi-asserted-by":"publisher","first-page":"232","DOI":"10.1038\/nature02632","volume":"430","author":"SY Lee","year":"2004","unstructured":"Lee, S.Y. & MacKinnon, R. A membrane-access mechanism of ion channel inhibition by voltage sensor toxins from spider venom. Nature 430, 232\u2013235 (2004).","journal-title":"Nature"},{"key":"BFnsmb1679_CR25","doi-asserted-by":"publisher","first-page":"6015","DOI":"10.1021\/bi0477034","volume":"44","author":"HJ Jung","year":"2005","unstructured":"Jung, H.J. et al. Solution structure and lipid membrane partitioning of VSTx1, an inhibitor of the KvAP potassium channel. Biochemistry 44, 6015\u20136023 (2005).","journal-title":"Biochemistry"},{"key":"BFnsmb1679_CR26","doi-asserted-by":"publisher","first-page":"857","DOI":"10.1038\/nature03873","volume":"436","author":"LR Phillips","year":"2005","unstructured":"Phillips, L.R. et al. Voltage-sensor activation with a tarantula toxin as cargo. Nature 436, 857\u2013860 (2005).","journal-title":"Nature"},{"key":"BFnsmb1679_CR27","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1016\/S0896-6273(00)80307-4","volume":"18","author":"KJ Swartz","year":"1997","unstructured":"Swartz, K.J. & MacKinnon, R. Mapping the receptor site for hanatoxin, a gating modifier of voltage-dependent K+ channels. Neuron 18, 675\u2013682 (1997b).","journal-title":"Neuron"},{"key":"BFnsmb1679_CR28","doi-asserted-by":"publisher","first-page":"33","DOI":"10.1085\/jgp.115.1.33","volume":"115","author":"Y Li-Smerin","year":"2000","unstructured":"Li-Smerin, Y., Hackos, D.H. & Swartz, K.J. \u03b1-Helical structural elements within the voltage-sensing domains of a K+ channel. J. Gen. Physiol. 115, 33\u201350 (2000a).","journal-title":"J. Gen. Physiol."},{"key":"BFnsmb1679_CR29","doi-asserted-by":"publisher","first-page":"205","DOI":"10.1085\/jgp.117.3.205","volume":"117","author":"Y Li-Smerin","year":"2001","unstructured":"Li-Smerin, Y. & Swartz, K.J. Helical structure of the COOH terminus of S3 and its contribution to the gating modifier toxin receptor in voltage-gated ion channels. J. Gen. Physiol. 117, 205\u2013218 (2001).","journal-title":"J. Gen. Physiol."},{"key":"BFnsmb1679_CR30","doi-asserted-by":"publisher","first-page":"1034","DOI":"10.2337\/diabetes.55.04.06.db05-0788","volume":"55","author":"J Herrington","year":"2006","unstructured":"Herrington, J. et al. Blockers of the delayed-rectifier potassium current in pancreatic \u03b2-cells enhance glucose-dependent insulin secretion. Diabetes 55, 1034\u20131042 (2006).","journal-title":"Diabetes"},{"key":"BFnsmb1679_CR31","doi-asserted-by":"publisher","first-page":"180","DOI":"10.1038\/nature01473","volume":"422","author":"V Ruta","year":"2003","unstructured":"Ruta, V., Jiang, Y., Lee, A., Chen, J. & MacKinnon, R. Functional analysis of an archaebacterial voltage-dependent K+ channel. Nature 422, 180\u2013185 (2003).","journal-title":"Nature"},{"key":"BFnsmb1679_CR32","doi-asserted-by":"publisher","first-page":"14734","DOI":"10.1021\/bi026546a","volume":"41","author":"RE Middleton","year":"2002","unstructured":"Middleton, R.E. et al. Two tarantula peptides inhibit activation of multiple sodium channels. Biochemistry 41, 14734\u201314747 (2002).","journal-title":"Biochemistry"},{"key":"BFnsmb1679_CR33","doi-asserted-by":"publisher","first-page":"673","DOI":"10.1085\/jgp.115.6.673","volume":"115","author":"Y Li-Smerin","year":"2000","unstructured":"Li-Smerin, Y. & Swartz, K.J. Localization and molecular determinants of the hanatoxin receptors on the voltage-sensing domain of a K+ channel. J. Gen. Physiol. 115, 673\u2013684 (2000).","journal-title":"J. Gen. Physiol."},{"key":"BFnsmb1679_CR34","doi-asserted-by":"publisher","first-page":"307","DOI":"10.1126\/science.7716527","volume":"268","author":"P Hidalgo","year":"1995","unstructured":"Hidalgo, P. & MacKinnon, R. Revealing the architecture of a K+ channel pore through mutant cycles with a peptide inhibitor. Science 268, 307\u2013310 (1995).","journal-title":"Science"},{"key":"BFnsmb1679_CR35","first-page":"478","volume":"248","author":"G Schreiber","year":"1995","unstructured":"Schreiber, G. & Fersht, A.R. Energetics of protein-protein interactions: analysis of the barnase-barstar interface by single mutations and double mutant cycles. J. Mol. Biol. 248, 478\u2013486 (1995).","journal-title":"J. Mol. Biol."},{"key":"BFnsmb1679_CR36","doi-asserted-by":"publisher","first-page":"7443","DOI":"10.1074\/jbc.275.11.7443","volume":"275","author":"JR Martens","year":"2000","unstructured":"Martens, J.R. et al. Differential targeting of Shaker-like potassium channels to lipid rafts. J. Biol. Chem. 275, 7443\u20137446 (2000).","journal-title":"J. Biol. Chem."},{"key":"BFnsmb1679_CR37","doi-asserted-by":"publisher","first-page":"419","DOI":"10.1085\/jgp.20028636","volume":"120","author":"TP Nguyen","year":"2002","unstructured":"Nguyen, T.P. & Horn, R. Movement and crevices around a sodium channel S3 segment. J. Gen. Physiol. 120, 419\u2013436 (2002).","journal-title":"J. Gen. Physiol."},{"key":"BFnsmb1679_CR38","doi-asserted-by":"publisher","first-page":"515","DOI":"10.1016\/S0896-6273(03)00646-9","volume":"40","author":"CS Gandhi","year":"2003","unstructured":"Gandhi, C.S., Clark, E., Loots, E., Pralle, A. & Isacoff, E.Y. The orientation and molecular movement of a K+ channel voltage-sensing domain. Neuron 40, 515\u2013525 (2003).","journal-title":"Neuron"},{"key":"BFnsmb1679_CR39","doi-asserted-by":"publisher","first-page":"687","DOI":"10.1085\/jgp.200609624","volume":"128","author":"RB Darman","year":"2006","unstructured":"Darman, R.B., Ivy, A.A., Ketty, V. & Blaustein, R.O. Constraints on voltage sensor movement in the Shaker K+ channel. J. Gen. Physiol. 128, 687\u2013699 (2006).","journal-title":"J. Gen. Physiol."},{"key":"BFnsmb1679_CR40","doi-asserted-by":"publisher","first-page":"623","DOI":"10.1016\/j.neuron.2006.10.005","volume":"52","author":"GJ Soler-Llavina","year":"2006","unstructured":"Soler-Llavina, G.J., Chang, T.H. & Swartz, K.J. Functional interactions at the interface between voltage-sensing and pore domains in the Shaker Kv channel. Neuron 52, 623\u2013634 (2006).","journal-title":"Neuron"},{"key":"BFnsmb1679_CR41","doi-asserted-by":"publisher","first-page":"897","DOI":"10.1126\/science.1116269","volume":"309","author":"SB Long","year":"2005","unstructured":"Long, S.B., Campbell, E.B. & Mackinnon, R. Crystal structure of a mammalian voltage-dependent Shaker family K+ channel. Science 309, 897\u2013903 (2005).","journal-title":"Science"},{"key":"BFnsmb1679_CR42","doi-asserted-by":"publisher","first-page":"11292","DOI":"10.1074\/jbc.M111679200","volume":"277","author":"R Mahfoud","year":"2002","unstructured":"Mahfoud, R. et al. Identification of a common sphingolipid-binding domain in Alzheimer, prion, and HIV-1 proteins. J. Biol. Chem. 277, 11292\u201311296 (2002).","journal-title":"J. Biol. Chem."},{"key":"BFnsmb1679_CR43","doi-asserted-by":"publisher","first-page":"927","DOI":"10.1016\/j.bbalip.2006.06.004","volume":"1761","author":"CF Snook","year":"2006","unstructured":"Snook, C.F., Jones, J.A. & Hannun, Y.A. Sphingolipid-binding proteins. Biochim. Biophys. Acta 1761, 927\u2013946 (2006).","journal-title":"Biochim. Biophys. Acta"},{"key":"BFnsmb1679_CR44","doi-asserted-by":"publisher","first-page":"665","DOI":"10.1016\/S0896-6273(00)80306-2","volume":"18","author":"KJ Swartz","year":"1997","unstructured":"Swartz, K.J. & MacKinnon, R. Hanatoxin modifies the gating of a voltage-dependent K+ channel through multiple binding sites. Neuron 18, 665\u2013673 (1997a).","journal-title":"Neuron"},{"key":"BFnsmb1679_CR45","doi-asserted-by":"publisher","first-page":"527","DOI":"10.1016\/S0896-6273(03)00636-6","volume":"40","author":"HC Lee","year":"2003","unstructured":"Lee, H.C., Wang, J.M. & Swartz, K.J. Interaction between extracellular Hanatoxin and the resting conformation of the voltage-sensor paddle in Kv channels. Neuron 40, 527\u2013536 (2003).","journal-title":"Neuron"}],"container-title":["Nature Structural &amp; Molecular Biology"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/nsmb.1679.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nsmb.1679","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/nsmb.1679.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T04:25:44Z","timestamp":1684470344000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/nsmb.1679"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,27]]},"references-count":45,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2009,10]]}},"alternative-id":["BFnsmb1679"],"URL":"https:\/\/doi.org\/10.1038\/nsmb.1679","relation":{},"ISSN":["1545-9993","1545-9985"],"issn-type":[{"value":"1545-9993","type":"print"},{"value":"1545-9985","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9,27]]}}}