{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T10:24:16Z","timestamp":1790850256185,"version":"4.1.0"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2012,9,6]],"date-time":"2012-09-06T00:00:00Z","timestamp":1346889600000},"content-version":"vor","delay-in-days":3505,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Obesity Research"],"published-print":{"date-parts":[[2003,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective<\/jats:italic>: To better understand the molecular basis of dietary obesity, we examined adipose tissue genes differentially expressed in an obesity model using DNA microarray analysis.<\/jats:p><jats:p><jats:italic>Research Methods and Procedures<\/jats:italic>: We assessed the expression level of over 12, 500 transcripts in epididymal fat pads from (cafeteria) obese and control rats with the aid of the array technology.<\/jats:p><jats:p><jats:italic>Results<\/jats:italic>: Cafeteria (obese) rats weighed 50% more and had 2.5\u2010fold higher levels of epididymal fat and elevated levels of circulating leptin. Adipose genes differentially expressed in obese and control rats were categorized into five groups: macronutrient metabolism, transcription factors, hormone receptor and signal transduction, redox and stress proteins, and cellular cytoskeleton. Interestingly, the expression levels of a number of genes involved in lipid metabolism such as glycerol\u20103\u2010phosphate dehydrogenase, stearoyl coenzyme A desaturase, together with the transcription factors implicated in adipocyte differentiation (CAAT\/enhancer binding protein\u2010\u03b1 and peroxisome proliferator\u2010activated receptor\u2010\u03b3), were significantly increased in obese animals compared with control. The most up\u2010regulated transcripts were the <jats:italic>ob<\/jats:italic> (49.2\u2010fold change) and the fatty acid\u2010binding protein genes (15.7\u2010 fold change). In contrast, genes related to redox and stress protein were generally down\u2010regulated in obese animals compared with the control.<\/jats:p><jats:p><jats:italic>Discussion<\/jats:italic>: Our study showed that in diet\u2010induced obesity, the expression levels of some important genes implicated in lipid metabolism were up\u2010regulated, whereas those related to redox and stress protein were down\u2010regulated in obese animals compared with control. This pattern of gene expression may occur in human obesity cases after high\u2010fat intake.<\/jats:p>","DOI":"10.1038\/oby.2003.30","type":"journal-article","created":{"date-parts":[[2008,4,18]],"date-time":"2008-04-18T13:36:28Z","timestamp":1208525788000},"page":"188-194","source":"Crossref","is-referenced-by-count":133,"title":["DNA Microarray Analysis of Genes Differentially Expressed in Diet\u2010Induced (Cafeteria) Obese Rats"],"prefix":"10.1002","volume":"11","author":[{"given":"Iciar P.","family":"L\u00f3pez","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amelia","family":"Marti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fermin I.","family":"Milagro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria de los Angeles","family":"Zulet","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Jesus","family":"Moreno\u2010Aliaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. Alfredo","family":"Martinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"De Miguel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9,6]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1079\/BJN2001410"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2000.41"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0029665100000458"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijo.0801097"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2000.30"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/4462"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/4434"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/4469"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.97.21.11371"},{"key":"e_1_2_7_11_2","doi-asserted-by":"crossref","first-page":"963","DOI":"10.1101\/gad.14.8.963","article-title":"Leptin\u2010specific patterns of gene expression in white adipose tissue","volume":"4","author":"Soukas A.","year":"2000","journal-title":"Genes Dev."},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M104421200"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2001.4733"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/oby.2001.65"},{"key":"e_1_2_7_15_2","first-page":"89","article-title":"The human obesity gene map: the 2001 update","volume":"8","author":"Rankinen T.","year":"2002","journal-title":"Obes Res."},{"key":"e_1_2_7_16_2","doi-asserted-by":"crossref","first-page":"2078","DOI":"10.1093\/jn\/131.8.2078","article-title":"Please pass the chips: genomic insights into obesity and diabetes","volume":"131","author":"Nadler S. 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