{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T08:36:31Z","timestamp":1791189391791,"version":"4.1.0"},"reference-count":70,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,9,6]],"date-time":"2012-09-06T00:00:00Z","timestamp":1346889600000},"content-version":"vor","delay-in-days":1955,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Objective:<\/jats:italic> High\u2010fat and marginally copper\u2010deficient diets impair heart function, leading to cardiac hypertrophy, increased lipid droplet volume, and compromised contractile function, resembling lipotoxic cardiac dysfunction. However, the combined effect of the two on cardiac function is unknown. This study was designed to examine the interaction between high\u2010fat and marginally copper\u2010deficient diets on cardiomyocyte contractile function.<\/jats:p><jats:p><jats:italic>Research Methods and Procedures:<\/jats:italic> Weanling male rats were fed diets incorporating a low\u2010 or high\u2010fat diet (10% or 45% of kcal from fat, respectively) with adequate (6 mg\/kg) or marginally deficient (1.5 mg\/kg) copper content for 12 weeks. Contractile function was determined with an IonOptix system including peak shortening (PS), time\u2010to\u2010PS, time\u2010to\u201090% relengthening, maximal velocity of shortening\/relengthening, and intracellular Ca<jats:sup>2+<\/jats:sup> ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>I<\/jats:sub>) rise and decay.<\/jats:p><jats:p><jats:italic>Results:<\/jats:italic> Neither dietary treatment affected blood pressure or glucose levels, although the high\u2010fat diet elicited obesity and glucose intolerance. Both diets depressed PS, maximal velocity of shortening\/relengthening, and intracellular Ca<jats:sup>2+<\/jats:sup> ([Ca<jats:sup>2+<\/jats:sup>]<jats:sub>I<\/jats:sub>) rise and prolonged time\u2010to\u201090% relengthening and Ca<jats:sup>2+<\/jats:sup> decay without an additive effect between the two. Ca<jats:sup>2+<\/jats:sup> sensitivity, apoptosis, lipid peroxidation, nitrosative damage, tissue ceramide, and triglyceride levels were unaffected by either diet or in combination. Phospholamban (PLB) but not sarco(endo)plasmic reticulum Ca<jats:sup>2+<\/jats:sup>\u2010ATPase was increased by both diets. Endothelial NO synthase was depressed with concurrent treatments. The electron transport chain was unaffected, although mitochondrial aconitase activity was inhibited by the high\u2010fat diet.<\/jats:p><jats:p><jats:italic>Discussion:<\/jats:italic> These data suggest that high\u2010fat and marginally copper deficient diets impaired cardiomyocyte contractile function and [Ca<jats:sup>2+<\/jats:sup>]<jats:sub>i<\/jats:sub> homeostasis, possibly through a similar mechanism, without obvious lipotoxicity, nitrosative damage, and apoptosis.<\/jats:p>","DOI":"10.1038\/oby.2007.146","type":"journal-article","created":{"date-parts":[[2008,7,16]],"date-time":"2008-07-16T08:06:11Z","timestamp":1216195571000},"page":"1242-1257","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Dietary Interaction of High Fat and Marginal Copper Deficiency on Cardiac Contractile Function"],"prefix":"10.1002","volume":"15","author":[{"given":"David P.","family":"Relling","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucy B.","family":"Esberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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