{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:25:06Z","timestamp":1790857506983,"version":"4.1.0"},"reference-count":66,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,9,6]],"date-time":"2012-09-06T00:00:00Z","timestamp":1346889600000},"content-version":"vor","delay-in-days":1528,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2008,7]]},"abstract":"<jats:p>\n                    The aim of this study was to determine whether amyloid precursor protein (\n                    <jats:italic>APP<\/jats:italic>\n                    ) is expressed in human adipose tissue, dysregulated in obesity, and related to insulin resistance and inflammation.\n                    <jats:italic>APP<\/jats:italic>\n                    expression was examined by microarray expression profiling of subcutaneous abdominal adipocytes (SAC) and cultured preadipocytes from obese and nonobese subjects. Quantitative real\u2010time PCR (QPCR) was performed to confirm differences in\n                    <jats:italic>APP<\/jats:italic>\n                    expression in SAC and to compare\n                    <jats:italic>APP<\/jats:italic>\n                    expression levels in adipose tissue, adipocytes, and stromal vascular cells (SVCs) from subcutaneous adipose tissue (SAT) and visceral adipose tissue (VAT) specimens. Adipose tissue samples were also examined by western blot and immunofluorescence confocal microscopy. Microarray studies demonstrated that\n                    <jats:italic>APP<\/jats:italic>\n                    mRNA expression levels were higher in SAC (\u223c2.5\u2010fold) and preadipocytes (\u223c1.4) from obese subjects. Real\u2010time PCR confirmed increased\n                    <jats:italic>APP<\/jats:italic>\n                    expression in SAC in a separate group of obese compared with nonobese subjects (\n                    <jats:italic>P<\/jats:italic>\n                    = 0.02).\n                    <jats:italic>APP<\/jats:italic>\n                    expression correlated to\n                    <jats:italic>in vivo<\/jats:italic>\n                    indices of insulin resistance independently of BMI and with the expression of proinflammatory genes, such as monocyte chemoattractant protein\u20101 (MCP\u20101) (\n                    <jats:italic>R<\/jats:italic>\n                    = 0.62,\n                    <jats:italic>P<\/jats:italic>\n                    = 0.004), macrophage inflammatory protein\u20101\u03b1 (MIP\u20101\u03b1) (\n                    <jats:italic>R<\/jats:italic>\n                    = 0.60,\n                    <jats:italic>P<\/jats:italic>\n                    = 0.005), and interleukin\u20106 (IL\u20106) (\n                    <jats:italic>R<\/jats:italic>\n                    = 0.71,\n                    <jats:italic>P<\/jats:italic>\n                    = 0.0005). Full\u2010length\n                    <jats:italic>APP<\/jats:italic>\n                    protein was detected in adipocytes by western blotting and APP and its cleavage peptides, A\u03b240 and A\u03b242, were observed in SAT and VAT by immunofluorescence confocal microscopy. In summary,\n                    <jats:italic>APP<\/jats:italic>\n                    is highly expressed in adipose tissue, upregulated in obesity, and expression levels correlate with insulin resistance and adipocyte cytokine expression levels. These data suggest a possible role for APP and\/or A\u03b2 in the development of obesity\u2010related insulin resistance and adipose tissue inflammation.\n                  <\/jats:p>","DOI":"10.1038\/oby.2008.267","type":"journal-article","created":{"date-parts":[[2008,5,15]],"date-time":"2008-05-15T10:47:03Z","timestamp":1210848423000},"page":"1493-1500","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":84,"title":["Amyloid Precursor Protein Expression Is Upregulated in Adipocytes in Obesity"],"prefix":"10.1002","volume":"16","author":[{"given":"Yong\u2010Ho","family":"Lee","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William G.","family":"Tharp","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rhonda 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