{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T11:40:17Z","timestamp":1790941217006,"version":"4.1.0"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,9,6]],"date-time":"2012-09-06T00:00:00Z","timestamp":1346889600000},"content-version":"vor","delay-in-days":1344,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:p>Roux\u2010en\u2010Y gastric bypass surgery (RYGBP) leads to improvements in satiety and obesity\u2010related comorbidities. The mechanism(s) underlying these improvements are not known but may be revealed in part by discovery proteomics. Therefore, fasting plasma was collected from 12 subjects (mean BMI &gt;45) during RYGBP and during a second procedure \u223c17 months later. Body weight, obesity\u2010related comorbidities, and medication use were decreased after RYGBP. Mass spectrometry\u2010based proteomic analysis was performed on a subset of seven samples using isobaric isotope\u2010coded affinity tags (four plex iTRAQ). Initial proteomic analysis (<jats:italic>n<\/jats:italic> = 7) quantified and identified hundreds of plasma proteins. Manual inspection of the data revealed a 2.6 \u00b1 0.5\u2010fold increase in apolipoprotein A\u2010IV (apo A\u2010IV, gene designation: APOA4), a \u223c46\u2010kDa glycoprotein synthesized mainly in the bypassed small bowel and liver after RYGBP. The change in apo A\u2010IV was significantly greater than other apolipoproteins. Immunoblot analysis of the full longitudinal sample set (<jats:italic>n<\/jats:italic> = 12) indicated even higher increases (8.3 \u00b1 0.2 fold) in apo A\u2010IV. Thus iTRAQ may underestimate the changes in protein concentrations compared to western blotting of apo A\u2010IV. Apo A\u2010IV inhibits gastric emptying and serves as a satiety factor whose synthesis and secretion are increased by the ingestion of dietary fat. It also possesses anti\u2010inflammatory and antiatherogenic properties. Based on these functions, we speculate changes in apo A\u2010IV may contribute to weight loss as well as the improvements in inflammation and cardiovascular disease after RYGBP. In addition, the findings provide evidence validating the use of iTRAQ proteomics in discovery\u2010based studies of post\u2010RYGBP improvements in obesity\u2010related medical comorbidities.<\/jats:p>","DOI":"10.1038\/oby.2008.428","type":"journal-article","created":{"date-parts":[[2008,10,23]],"date-time":"2008-10-23T14:24:28Z","timestamp":1224771868000},"page":"46-52","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["Apolipoprotein A\u2010IV, a Putative Satiety\/Antiatherogenic Factor, Rises After Gastric Bypass"],"prefix":"10.1002","volume":"17","author":[{"given":"Derek M.","family":"Culnan","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert N.","family":"Cooney","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruce","family":"Stanley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher J.","family":"Lynch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9,6]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1521-6918(04)00064-2"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa066603"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12013-007-0020-y"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.soard.2007.02.013"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.soard.2006.12.004"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1381\/096089204772787220"},{"key":"e_1_2_6_8_2","first-page":"24","article-title":"[The \u201cSwedish Obese Subjects\u201d (SOS) Study. 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