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To identify pituitary mediators for ADX\u2010induced physiological alterations, such as weight loss and hypotension, we investigated the effect of ADX on the pituitary transcriptome using serial analysis of gene expression (SAGE). SAGE method is based on isolation of short sequence tags, which usually correspond to unique mRNA species. The SAGE libraries were constructed from pituitary gland of intact (<jats:italic>n<\/jats:italic> = 51) and ADX (<jats:italic>n<\/jats:italic> = 12) mice. Thirty\u2010one transcripts were differentially expressed between intact and ADX. Three transcripts encoding for proopiomelanocortin and three other transcripts involved in regulation of hormone secretion (neuromedin B, proprotein convertase subtilisin\/kexin type 2, and IA\u20102) were induced by ADX. In addition, ADX increased the expression levels of genes encoding for cation extracellular matrix (matrix \u03b3\u2010carboxyglutamate protein) and transport (solute carrier family 22 member 17). Conversely, ADX downregulated two transcripts involved in mitochondrial oxidative phosphorylation (nicotinamide adenine dinucleotide (NADH) dehydrogenase 3 and cytochrome c oxidase 3). Moreover, ADX significantly modulated the expression levels of one gene with uncharacterized function and 20 novel transcripts. This study reveals alterations of pituitary gene expressions that may be associated with ADX\u2010induced physiological changes including weight loss.<\/jats:p>","DOI":"10.1038\/oby.2008.484","type":"journal-article","created":{"date-parts":[[2008,11,18]],"date-time":"2008-11-18T12:35:03Z","timestamp":1227011703000},"page":"114-120","update-policy":"http:\/\/dx.doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Regulation of Pituitary Gene Expression by Adrenalectomy"],"prefix":"10.1002","volume":"17","author":[{"given":"Yuichiro","family":"Nishida","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mayumi","family":"Yoshioka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chester 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