{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T16:58:42Z","timestamp":1785862722384,"version":"3.56.0"},"reference-count":22,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,12,31]],"date-time":"2012-12-31T00:00:00Z","timestamp":1356912000000},"content-version":"vor","delay-in-days":1095,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2010,1]]},"abstract":"<jats:p>\n                    We have previously shown that combined amylin + leptin agonism elicits synergistic weight loss in diet\u2010induced obese (DIO) rats. Here, we assessed the comparative efficacy of amylin, leptin, or amylin + leptin in the maintenance of amylin + leptin\u2013mediated weight loss. DIO rats pretreated with the combination of rat amylin (50 \u00b5g\/kg\/day) and murine leptin (125 \u00b5g\/kg\/day) for 4 weeks were subsequently infused with either vehicle, amylin, leptin, or amylin + leptin for an additional 4 weeks. Food intake, body weight, body composition, plasma parameters, and the expression of key metabolic genes in liver and white adipose tissue (WAT) were assessed. Amylin + leptin treatment (weeks 0\u20134) reduced body weight to 87.5% of baseline. Rats subsequently maintained on vehicle or leptin regained all weight (to 104.2 and 101.2% of baseline, respectively), those maintained on amylin had partial weight regain (97.0%). By contrast, weight loss was largely maintained with continued amylin + leptin treatment (91.4%), associated with a 10% decrease in adiposity. Cumulative food intake (weeks 5\u20138) was reduced by amylin and amylin + leptin, but not by leptin alone. Amylin + leptin, but not amylin or leptin alone, reduced plasma triglycerides (by 55%), total cholesterol (by 19%), and insulin (by 57%) compared to vehicle. Amylin + leptin also reduced hepatic stearoyl\u2010CoA desaturase\u20101 (\n                    <jats:italic>Scd1<\/jats:italic>\n                    ) mRNA, and increased WAT mRNA levels of adiponectin, fatty acid synthase (\n                    <jats:italic>Fasn<\/jats:italic>\n                    ), and lipoprotein lipase (\n                    <jats:italic>Lpl<\/jats:italic>\n                    ). We conclude that, in DIO rats, maintenance of amylin + leptin\u2013mediated weight loss requires continued treatment with both agonists, and is accompanied by sustained improvements in body composition, and indices of lipid metabolism and insulin sensitivity.\n                  <\/jats:p>","DOI":"10.1038\/oby.2009.187","type":"journal-article","created":{"date-parts":[[2009,6,18]],"date-time":"2009-06-18T10:28:32Z","timestamp":1245320912000},"page":"21-26","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":83,"title":["Interaction of Leptin and Amylin in the Long\u2010term Maintenance of Weight Loss in Diet\u2010induced Obese Rats"],"prefix":"10.1002","volume":"18","author":[{"given":"James L.","family":"Trevaskis","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunli","family":"Lei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joy E.","family":"Koda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Weyer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David G.","family":"Parkes","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan D.","family":"Roth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,12,31]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2174\/157339908784220741"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0706473105"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2008-0770"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00393.2003"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0306-4522(01)00142-7"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/382250a0"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI25977"},{"key":"e_1_2_7_9_1","first-page":"2583","article-title":"Leptin reverses weight loss\u2010induced changes in regional neural activity responses to visual food stimuli","volume":"118","author":"Rosenbaum 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