{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T20:20:38Z","timestamp":1790713238019,"version":"4.1.0"},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2012,9,6]],"date-time":"2012-09-06T00:00:00Z","timestamp":1346889600000},"content-version":"vor","delay-in-days":249,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Obesity"],"published-print":{"date-parts":[[2012,1]]},"abstract":"<jats:p>The present study examined whether the perinephric and epididymal visceral fat (PEVF) depot under short\u2010term excess nutrient protected the liver by trapping nutrient\u2010derived nonesterified free fatty acids (NEFAs) or had deleterious effects on hepatic triglycerides (TGs) accumulation and insulin resistance due to adipokine secretion. Young rats pre\u2010emptively underwent surgical PEVF removal or sham operations and were fed with either high\u2010fat diet (HFD) (PEVF\u2010HFD) or regular chow (RC) (PEVF\u2010RC) for 3 days. Insulin sensitivity was measured by hyperinsulinemic\u2010euglycemic clamp. Liver TG, serum NEFA, and fat\u2010derived adipokines were assessed. Insulin and lipogenesis signaling were assessed by western blots. Pre\u2010emptive PEVF removal significantly decreases insulin\u2010induced suppression of hepatic glucose production (HGP) both in RC and in HFD\u2010fed rats. In accordance with the clamp results, hepatic TG accumulation is also significantly reduced by PEVF excision both in RC and HFD\u2010fed rats. These results are further validated by insulin signaling results, which show that pre\u2010emptive PEVF removal increases phosphorylation of hepatic Akt, irrespective of diet. Notably, high levels of serum leptin induced by HFD are significantly reduced by pre\u2010emptive PEVF excision. Additionally, expression of lipogenic enzyme p\u2010acetyl\u2010CoA\u2010carboxylase, denoting reduced lipogenesis, is increased in the PEVF\u2010HFD rats. In conclusion, PEVF has a deleterious effect on the liver as a source of insulin resistance\u2010inducing adipokines irrespective of diet, and does not serve as a buffer for excess nutrients.<\/jats:p>","DOI":"10.1038\/oby.2011.261","type":"journal-article","created":{"date-parts":[[2011,8,4]],"date-time":"2011-08-04T13:32:49Z","timestamp":1312464769000},"page":"151-156","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Perinephric and Epididymal Fat Affect Hepatic Metabolism in Rats"],"prefix":"10.1002","volume":"20","author":[{"given":"Shani","family":"Ben\u2010Shlomo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francine H.","family":"Einstein","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabel","family":"Zvibel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dikla","family":"Atias","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amir","family":"Shlomai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zamir","family":"Halpern","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nir","family":"Barzilai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sigal","family":"Fishman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,9,6]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23280"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20920"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-002-0873-y"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1677\/JOE-10-0272"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000165803.47776.38"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.23046"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabet.2008.04.001"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-007-0640-1"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2010-0340"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0410-400"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)60408-4"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.2337\/diacare.14.12.1132"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.280.21.1843"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI24930"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.51.10.2951"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1096\/fj.06-6557com"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11892-010-0122-6"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/568343"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.50.12.2786"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215001"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.00128.2002"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ijo.0801871"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.04.056"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11695-008-9660-2"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1055\/s-2002-38250"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.2337\/diabetes.54.3.672"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diabet.2008.04.001"}],"container-title":["Obesity"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Foby.2011.261","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1038%2Foby.2011.261","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1038\/oby.2011.261","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T19:19:07Z","timestamp":1697570347000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1038\/oby.2011.261"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,1]]},"references-count":27,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2012,1]]}},"alternative-id":["10.1038\/oby.2011.261"],"URL":"https:\/\/doi.org\/10.1038\/oby.2011.261","archive":["Portico"],"relation":{},"ISSN":["1930-7381","1930-739X"],"issn-type":[{"value":"1930-7381","type":"print"},{"value":"1930-739X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,1]]},"assertion":[{"value":"2012-09-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}