{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,26]],"date-time":"2026-03-26T12:08:11Z","timestamp":1774526891085,"version":"3.50.1"},"reference-count":27,"publisher":"Springer Science and Business Media LLC","issue":"3","license":[{"start":{"date-parts":[[2012,11,30]],"date-time":"2012-11-30T00:00:00Z","timestamp":1354233600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Pediatr Res"],"published-print":{"date-parts":[[2013,3]]},"DOI":"10.1038\/pr.2012.176","type":"journal-article","created":{"date-parts":[[2012,11,30]],"date-time":"2012-11-30T17:02:55Z","timestamp":1354294975000},"page":"355-361","source":"Crossref","is-referenced-by-count":19,"title":["RT-PCR detection of respiratory pathogens in newborn children admitted to a neonatal medium care unit"],"prefix":"10.1038","volume":"73","author":[{"given":"Patrick M.","family":"Smit","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne M.","family":"Pronk","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos C.","family":"Kaandorp","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivier","family":"Weijer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fanny N.","family":"Lauw","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul H.M.","family":"Smits","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eric C.J.","family":"Claas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan W.","family":"Mulder","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jos H.","family":"Beijnen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dees P.M.","family":"Brandjes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2012,11,30]]},"reference":[{"key":"BFpr2012176_CR1","doi-asserted-by":"crossref","first-page":"296","DOI":"10.1542\/peds.84.2.296","volume":"84","author":"JM Jason","year":"1989","unstructured":"Jason JM . Infectious disease-related deaths of low birth weight infants, United States, 1968 to 1982. Pediatrics 1989;84:296\u2013303.","journal-title":"Pediatrics"},{"key":"BFpr2012176_CR2","doi-asserted-by":"publisher","first-page":"240","DOI":"10.1056\/NEJMoa012657","volume":"347","author":"BJ Stoll","year":"2002","unstructured":"Stoll BJ, Hansen N, Fanaroff AA, et al. Changes in pathogens causing early-onset sepsis in very-low-birth-weight infants. N Engl J Med 2002;347:240\u20137.","journal-title":"N Engl J Med"},{"key":"BFpr2012176_CR3","unstructured":"Klein JO, Marcy SM . Bacterial sepsis and meningitis. In: Remington JS, Klein JO, eds. Infectious Diseases of the Fetus and Newborn Infant. Philadelphia, Pennsylvania: WB Saunders, 1995:835\u201390."},{"key":"BFpr2012176_CR4","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1542\/peds.69.1.40","volume":"69","author":"S Voora","year":"1982","unstructured":"Voora S, Srinivasan G, Lilien LD, Yeh TF, Pildes RS . Fever in full-term newborns in the first four days of life. Pediatrics 1982;69:40\u20134.","journal-title":"Pediatrics"},{"key":"BFpr2012176_CR5","unstructured":"Korpela J, Campbell J, Singh N . Healthcare-associated infections. In: MacDonald M, Seshia M, Mullett M, eds. Avery\u2019s Neonatology. Philadelphia, Pennsylvania: Wolters Kluwer\/Lippincott Williams & Wilkins, 2005:1366\u20137."},{"key":"BFpr2012176_CR6","unstructured":"Schelonka R, Freij B, McCracken G Jr . Bacterial and fungal infections. In: MacDonald M, Seshia M, Mullett M, eds. Avery\u2019s Neonatology. Philadelphia, Pennsylvania: Wolters Kluwer\/Lippincott Williams & Wilkins, 2005:1235\u20138."},{"key":"BFpr2012176_CR7","doi-asserted-by":"publisher","first-page":"74","DOI":"10.1128\/CMR.00032-09","volume":"23","author":"JS Tregoning","year":"2010","unstructured":"Tregoning JS, Schwarze J . Respiratory viral infections in infants: causes, clinical symptoms, virology, and immunology. Clin Microbiol Rev 2010;23:74\u201398.","journal-title":"Clin Microbiol Rev"},{"key":"BFpr2012176_CR8","doi-asserted-by":"publisher","first-page":"901","DOI":"10.1097\/01.inf.0000180471.03702.7f","volume":"24","author":"MA Verboon-Maciolek","year":"2005","unstructured":"Verboon-Maciolek MA, Krediet TG, Gerards LJ, Fleer A, van Loon TM . Clinical and epidemiologic characteristics of viral infections in a neonatal intensive care unit during a 12-year period. Pediatr Infect Dis J 2005;24:901\u20134.","journal-title":"Pediatr Infect Dis J"},{"key":"BFpr2012176_CR9","doi-asserted-by":"publisher","first-page":"341","DOI":"10.1080\/jmf.13.5.341.350","volume":"13","author":"RA Vieira","year":"2003","unstructured":"Vieira RA, Diniz EM, Vaz FA . Clinical and laboratory study of newborns with lower respiratory tract infection due to respiratory viruses. J Matern Fetal Neonatal Med 2003;13:341\u201350.","journal-title":"J Matern Fetal Neonatal Med"},{"key":"BFpr2012176_CR10","doi-asserted-by":"publisher","first-page":"120","DOI":"10.1136\/adc.59.2.120","volume":"59","author":"PT Rudd","year":"1984","unstructured":"Rudd PT, Carrington D . A prospective study of chlamydial, mycoplasmal, and viral infections in a neonatal intensive care unit. Arch Dis Child 1984;59:120\u20135.","journal-title":"Arch Dis Child"},{"key":"BFpr2012176_CR11","doi-asserted-by":"publisher","first-page":"680","DOI":"10.1097\/01.inf.0000226912.88900.a3","volume":"25","author":"MM Kusel","year":"2006","unstructured":"Kusel MM, de Klerk NH, Holt PG, Kebadze T, Johnston SL, Sly PD . Role of respiratory viruses in acute upper and lower respiratory tract illness in the first year of life: a birth cohort study. Pediatr Infect Dis J 2006;25:680\u20136.","journal-title":"Pediatr Infect Dis J"},{"key":"BFpr2012176_CR12","doi-asserted-by":"publisher","first-page":"e18928","DOI":"10.1371\/journal.pone.0018928","volume":"6","author":"PG Bezerra","year":"2011","unstructured":"Bezerra PG, Britto MC, Correia JB, et al. Viral and atypical bacterial detection in acute respiratory infection in children under five years. PLoS ONE 2011;6:e18928.","journal-title":"PLoS ONE"},{"key":"BFpr2012176_CR13","doi-asserted-by":"publisher","first-page":"1305","DOI":"10.1007\/s10096-009-0780-y","volume":"28","author":"N De Vos","year":"2009","unstructured":"De Vos N, Vankeerberghen A, Vaeyens F, Van Vaerenbergh K, Boel A, De Beenhouwer H . Simultaneous detection of human bocavirus and adenovirus by multiplex real-time PCR in a Belgian paediatric population. Eur J Clin Microbiol Infect Dis 2009;28:1305\u201310.","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"BFpr2012176_CR14","doi-asserted-by":"publisher","first-page":"1498","DOI":"10.1002\/jmv.20725","volume":"78","author":"F Freymuth","year":"2006","unstructured":"Freymuth F, Vabret A, Cuvillon-Nimal D, et al. Comparison of multiplex PCR assays and conventional techniques for the diagnostic of respiratory virus infections in children admitted to hospital with an acute respiratory illness. J Med Virol 2006;78:1498\u2013504.","journal-title":"J Med Virol"},{"key":"BFpr2012176_CR15","doi-asserted-by":"publisher","first-page":"R61","DOI":"10.1186\/cc4895","volume":"10","author":"AC van de Pol","year":"2006","unstructured":"van de Pol AC, Wolfs TF, Jansen NJ, van Loon AM, Rossen JW . Diagnostic value of real-time polymerase chain reaction to detect viruses in young children admitted to the paediatric intensive care unit with lower respiratory tract infection. Crit Care 2006;10:R61.","journal-title":"Crit Care"},{"key":"BFpr2012176_CR16","doi-asserted-by":"publisher","first-page":"2631","DOI":"10.1128\/JCM.02094-10","volume":"49","author":"RR Jansen","year":"2011","unstructured":"Jansen RR, Wieringa J, Koekkoek SM, et al. Frequent detection of respiratory viruses without symptoms: toward defining clinically relevant cutoff values. J Clin Microbiol 2011;49:2631\u20136.","journal-title":"J Clin Microbiol"},{"key":"BFpr2012176_CR17","doi-asserted-by":"publisher","first-page":"2126","DOI":"10.1128\/JCM.02553-06","volume":"45","author":"PF Wright","year":"2007","unstructured":"Wright PF, Deatly AM, Karron RA, et al. Comparison of results of detection of rhinovirus by PCR and viral culture in human nasal wash specimens from subjects with and without clinical symptoms of respiratory illness. J Clin Microbiol 2007;45:2126\u20139.","journal-title":"J Clin Microbiol"},{"key":"BFpr2012176_CR18","doi-asserted-by":"publisher","first-page":"1487","DOI":"10.1056\/NEJMoa052632","volume":"357","author":"H Bisgaard","year":"2007","unstructured":"Bisgaard H, Hermansen MN, Buchvald F, et al. Childhood asthma after bacterial colonization of the airway in neonates. N Engl J Med 2007;357:1487\u201395.","journal-title":"N Engl J Med"},{"key":"BFpr2012176_CR19","doi-asserted-by":"publisher","first-page":"588","DOI":"10.1056\/NEJMoa0804877","volume":"360","author":"CB Hall","year":"2009","unstructured":"Hall CB, Weinberg GA, Iwane MK, et al. The burden of respiratory syncytial virus infection in young children. N Engl J Med 2009;360:588\u201398.","journal-title":"N Engl J Med"},{"key":"BFpr2012176_CR20","doi-asserted-by":"publisher","first-page":"788","DOI":"10.1097\/INF.0b013e3181710990","volume":"27","author":"J Figueras-Aloy","year":"2008","unstructured":"Figueras-Aloy J, Carbonell-Estrany X, Quero-Jim\u00e9nez J, et al. FLIP-2 Study: risk factors linked to respiratory syncytial virus infection requiring hospitalization in premature infants born in Spain at a gestational age of 32 to 35 weeks. Pediatr Infect Dis J 2008;27:788\u201393.","journal-title":"Pediatr Infect Dis J"},{"issue":"Suppl 5","key":"BFpr2012176_CR21","doi-asserted-by":"publisher","first-page":"S118","DOI":"10.1067\/S0022-3476(03)00511-0","volume":"143","author":"EA Simoes","year":"2003","unstructured":"Simoes EA . Environmental and demographic risk factors for respiratory syncytial virus lower respiratory tract disease. J Pediatr 2003;143:Suppl 5:S118\u201326.","journal-title":"J Pediatr"},{"key":"BFpr2012176_CR22","doi-asserted-by":"publisher","first-page":"F4","DOI":"10.1136\/adc.2009.178483","volume":"96","author":"B Muller-Pebody","year":"2011","unstructured":"Muller-Pebody B, Johnson AP, Heath PT, Gilbert RE, Henderson KL, Sharland M . Empirical treatment of neonatal sepsis: are the current guidelines adequate? Arch Dis Child Fetal Neonatal Ed 2011;96:F4\u20138.","journal-title":"Arch Dis Child Fetal Neonatal Ed"},{"key":"BFpr2012176_CR23","doi-asserted-by":"publisher","first-page":"405","DOI":"10.2165\/00003495-199958030-00003","volume":"58","author":"V Fanos","year":"1999","unstructured":"Fanos V, Dall\u2019Agnola A . Antibiotics in neonatal infections: a review. Drugs 1999;58:405\u201327.","journal-title":"Drugs"},{"key":"BFpr2012176_CR24","doi-asserted-by":"publisher","first-page":"607","DOI":"10.1007\/s00431-005-1705-0","volume":"164","author":"B Ehlken","year":"2005","unstructured":"Ehlken B, Ihorst G, Lippert B, et al. Economic impact of community-acquired and nosocomial lower respiratory tract infections in young children in Germany. Eur J Pediatr 2005;164:607\u201315.","journal-title":"Eur J Pediatr"},{"key":"BFpr2012176_CR25","doi-asserted-by":"publisher","first-page":"688","DOI":"10.1038\/sj.jp.7211597","volume":"26","author":"BS Brozanski","year":"2006","unstructured":"Brozanski BS, Jones JG, Krohn MJ, Jordan JA . Use of polymerase chain reaction as a diagnostic tool for neonatal sepsis can result in a decrease in use of antibiotics and total neonatal intensive care unit length of stay. J Perinatol 2006;26:688\u201392.","journal-title":"J Perinatol"},{"key":"BFpr2012176_CR26","doi-asserted-by":"publisher","first-page":"e1113","DOI":"10.1542\/peds.2010-2779","volume":"128","author":"JO Wishaupt","year":"2011","unstructured":"Wishaupt JO, Russcher A, Smeets LC, Versteegh FG, Hartwig NG . Clinical impact of RT-PCR for pediatric acute respiratory infections: a controlled clinical trial. Pediatrics 2011;128:e1113\u201320.","journal-title":"Pediatrics"},{"key":"BFpr2012176_CR27","doi-asserted-by":"publisher","first-page":"1564","DOI":"10.1128\/JCM.42.4.1564-1569.2004","volume":"42","author":"KE Templeton","year":"2004","unstructured":"Templeton KE, Scheltinga SA, Beersma MF, Kroes AC, Claas EC . Rapid and sensitive method using multiplex real-time PCR for diagnosis of infections by influenza a and influenza B viruses, respiratory syncytial virus, and parainfluenza viruses 1, 2, 3, and 4. J Clin Microbiol 2004;42:1564\u20139.","journal-title":"J Clin Microbiol"}],"container-title":["Pediatric Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/pr2012176.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/pr2012176","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/pr2012176.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,19]],"date-time":"2023-05-19T04:56:35Z","timestamp":1684472195000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/pr2012176"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,11,30]]},"references-count":27,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,3]]}},"alternative-id":["BFpr2012176"],"URL":"https:\/\/doi.org\/10.1038\/pr.2012.176","relation":{},"ISSN":["0031-3998","1530-0447"],"issn-type":[{"value":"0031-3998","type":"print"},{"value":"1530-0447","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,11,30]]}}}