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Initial studies found that it was actively regulated in cancer cells, suggesting its involvement in the process of carcinogenesis. However, the contribution of 6\u2009mA in tongue squamous cell carcinoma (TSCC) still remains uncharacterized. In this study, a pan-cancer type analysis was first performed, which revealed enhanced 6\u2009mA metabolism in diverse cancer types. The study was then focused on the regulation of 6\u2009mA metabolism, as well as its effects on TSCC cells. To these aspects, genome 6\u2009mA level was found greatly increased in TSCC tissues and cultured cells. By knocking down 6\u2009mA methylases <jats:italic>N6AMT1<\/jats:italic> and <jats:italic>METTL4<\/jats:italic>, the level of genomic 6\u2009mA was decreased in TSCC cells. This led to suppressed colony formation and cell migration. By contrast, knockdown of 6\u2009mA demethylase <jats:italic>ALKBH1<\/jats:italic> resulted in an increased 6\u2009mA level, enhanced colony formation, and cell migration. Further study suggested that regulation of the NF-\u03baB pathway might contribute to the enhanced migration of TSCC cells. Therefore, in the case of TSCC, we have shown that genomic 6\u2009mA modification is involved in the proliferation and migration of cancer cells.<\/jats:p>","DOI":"10.1038\/s41368-022-00161-9","type":"journal-article","created":{"date-parts":[[2022,2,17]],"date-time":"2022-02-17T07:02:49Z","timestamp":1645081369000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["The enhanced genomic 6\u2009mA metabolism contributes to the proliferation and migration of TSCC cells"],"prefix":"10.1038","volume":"14","author":[{"given":"Lei","family":"Xi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fangming","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinghui","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiyang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhenxi","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan","family":"Du","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,2,17]]},"reference":[{"key":"161_CR1","doi-asserted-by":"publisher","first-page":"879","DOI":"10.1016\/j.cell.2015.04.010","volume":"161","author":"Y Fu","year":"2015","unstructured":"Fu, Y. et al. 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