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Weakly activating\n                    <jats:italic>JAK2<\/jats:italic>\n                    germline variants have been associated with MPN risk, but the underlying mechanisms remain unclear. We previously identified the\n                    <jats:italic>JAK2<\/jats:italic>\n                    -R1063H germline variant, which contributes to hereditary MPN and increased disease severity in essential thrombocythemia. Here, we studied alterations in hematopoiesis in\n                    <jats:italic>Jak2<\/jats:italic>\n                    -R1063H knock-in mice. The\n                    <jats:italic>Jak2<\/jats:italic>\n                    -R1063H mouse cohort exhibited increased mortality, stimulated thrombopoiesis and elevated D-dimers levels, indicative of thrombotic complications. Bone marrow analysis revealed myeloid bias, enhanced megakaryopoiesis and activation of inflammatory signaling. Transcriptional and functional assays of hematopoietic stem cells suggested their accelerated aging and functional decline. The Egr1 transcriptional network, including the\n                    <jats:italic>Thbs1<\/jats:italic>\n                    gene, progressively increased in aging mice, reinforcing alterations initiated by Jak2\/Stat signaling. In murine acute myelogenous leukemia models, the\n                    <jats:italic>Jak2<\/jats:italic>\n                    -R1063H cooperated with a driver oncogene in promoting leukemogenesis. Germline\n                    <jats:italic>JAK2<\/jats:italic>\n                    -R1063H was found in 10 of 200 MPN patients from local hematology centers, with a higher minor allele frequency compared to healthy controls. Patients harboring\n                    <jats:italic>JAK2<\/jats:italic>\n                    -R1063H variant exhibited an increased incidence of thrombotic complications and disease progression with shortened survival. In conclusion, our findings identify the\n                    <jats:italic>JAK2<\/jats:italic>\n                    -R1063H germline variant as a risk factor for MPN development, thrombotic complications, and leukemic transformation.\n                  <\/jats:p>","DOI":"10.1038\/s41375-025-02737-w","type":"journal-article","created":{"date-parts":[[2025,8,21]],"date-time":"2025-08-21T11:57:59Z","timestamp":1755777479000},"page":"2745-2757","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Germline Jak2-R1063H mutation interferes with normal hematopoietic development and increases risk of thrombosis and leukemic transformation"],"prefix":"10.1038","volume":"39","author":[{"given":"Veronika","family":"Zimolova","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Monika","family":"Burocziova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"Berkova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srdjan","family":"Grusanovic","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Gursky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-4400-3654","authenticated-orcid":false,"given":"Lubos","family":"Janotka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petr","family":"Kasparek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0204-7502","authenticated-orcid":false,"given":"Alena","family":"Pecinova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4770-1229","authenticated-orcid":false,"given":"David","family":"Kundrat","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1752-3612","authenticated-orcid":false,"given":"Dusan","family":"Hrckulak","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6569-6836","authenticated-orcid":false,"given":"Jakub","family":"Onhajzer","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivana","family":"Jeziskova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucie","family":"Nekvindova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6460-2471","authenticated-orcid":false,"given":"Barbora","family":"Weinbergerova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7136-2680","authenticated-orcid":false,"given":"Sarka","family":"Pospisilova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1269-6282","authenticated-orcid":false,"given":"Michael","family":"Doubek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meritxell","family":"Alberich-Jorda","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladimir","family":"Korinek","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0202-245X","authenticated-orcid":false,"given":"Vladimir","family":"Divoky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2845-8692","authenticated-orcid":false,"given":"Lucie","family":"Lanikova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2025,8,21]]},"reference":[{"key":"2737_CR1","doi-asserted-by":"publisher","first-page":"273","DOI":"10.1111\/j.1600-065X.2008.00754.x","volume":"228","author":"K Ghoreschi","year":"2009","unstructured":"Ghoreschi K, Laurence A, O\u2019Shea JJ. 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