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Recent studies on RNA methylation mainly focus on the m\n                    <jats:sup>6<\/jats:sup>\n                    A modification of mRNA, but very little is known about the m\n                    <jats:sup>5<\/jats:sup>\n                    C modification. NSUN2 is an RNA methyltransferase responsible for the m\n                    <jats:sup>5<\/jats:sup>\n                    C modification of multiple RNAs. In this study, we knocked down the NSUN2 gene in HepG2 cells by CRISPR\/Cas9 technology and performed high-throughput RNA-BisSeq. An important tumor-related lncRNA H19 was identified to be targeted by NSUN2. Studies have shown that the expression of\n                    <jats:italic>H19<\/jats:italic>\n                    lncRNA is abnormally elevated and has a carcinogenic effect in many types of tumors. Our results demonstrated that m\n                    <jats:sup>5<\/jats:sup>\n                    C modification of\n                    <jats:italic>H19<\/jats:italic>\n                    lncRNA can increase its stability. Interestingly, m\n                    <jats:sup>5<\/jats:sup>\n                    C-modified\n                    <jats:italic>H19<\/jats:italic>\n                    lncRNA can be specifically bound by G3BP1, a well-known oncoprotein which further leads to MYC accumulation. This may be a novel mechanism by which lncRNA H19 exerts its oncogenic effect. Besides, both the m\n                    <jats:sup>5<\/jats:sup>\n                    C methylation level and the expression level of\n                    <jats:italic>H19<\/jats:italic>\n                    lncRNA in hepatocellular carcinoma tissues were significantly higher than those in adjacent non-cancer tissues, which were closely associated with poor differentiation of hepatocellular carcinoma (HCC). In conclusion, we found that\n                    <jats:italic>H19<\/jats:italic>\n                    RNA is a specific target for the NSUN2 modifier. The m\n                    <jats:sup>5<\/jats:sup>\n                    C-modified\n                    <jats:italic>H19<\/jats:italic>\n                    lncRNA may promote the occurrence and development of tumors by recruiting the G3BP1 oncoprotein. Our findings may provide a potential target and biomarker for the diagnosis and treatment of HCC.\n                  <\/jats:p>","DOI":"10.1038\/s41388-020-01475-w","type":"journal-article","created":{"date-parts":[[2020,9,25]],"date-time":"2020-09-25T07:03:08Z","timestamp":1601017388000},"page":"6906-6919","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":235,"title":["Aberrant NSUN2-mediated m5C modification of H19 lncRNA is associated with poor differentiation of hepatocellular carcinoma"],"prefix":"10.1038","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0144-3594","authenticated-orcid":false,"given":"Zhen","family":"Sun","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Songlei","family":"Xue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiying","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Xu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuming","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shihao","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yangyang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9445-9984","authenticated-orcid":false,"given":"Mingzhou","family":"Guo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5139-5370","authenticated-orcid":false,"given":"Hengmi","family":"Cui","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,9,25]]},"reference":[{"key":"1475_CR1","doi-asserted-by":"crossref","first-page":"556","DOI":"10.1038\/s41586-018-0538-8","volume":"561","author":"J Choe","year":"2018","unstructured":"Choe J, Lin S, Zhang W, Liu Q, Wang L, Ramirez-Moya J, et al. mRNA circularization by METTL3-eIF3h enhances translation and promotes oncogenesis. 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