{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T14:00:47Z","timestamp":1788012047058,"version":"build-2784847793"},"reference-count":47,"publisher":"Springer Science and Business Media LLC","issue":"7","license":[{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T00:00:00Z","timestamp":1717113600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Pediatr Res"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n          <jats:sec>\n            <jats:title>Background<\/jats:title>\n            <jats:p>Infants born preterm have a higher incidence of neurological deficits. A key step in finding effective treatments is to identify biomarkers that reliably predict outcome.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Methods<\/jats:title>\n            <jats:p>Following umbilical cord occlusion (UCO) in pregnant sheep, whole fetal blood RNA was sequenced pre- and post-UCO, brain injury outcome was determined by battery of neuropathology scoring and the transcriptome signature correlated to the degree of brain injury. Additionally, we developed a novel analytical procedure to deduce cell blood composition over time.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Results<\/jats:title>\n            <jats:p>Sixty-one genes were identified with significant altered expression after UCO. In pre-UCO blood, the level of three mRNAs (<jats:italic>Trex2<\/jats:italic>, <jats:italic>Znf280b<\/jats:italic>, novel miRNA) and in post-UCO, four mRNAs (<jats:italic>Fam184a<\/jats:italic>, <jats:italic>Angptl2<\/jats:italic>, novel lincRNA and an unknown protein-coding gene) were associated to brain injury (FDR\u2009&lt;\u20090.01). Several of these mRNAs are related to inflammation and angiogenesis. Pathway analysis highlighted genes playing a role in perinatal death and growth failure. Results also indicate that several leukocyte populations undergo significant changes after UCO.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Conclusion<\/jats:title>\n            <jats:p>We have used a whole transcriptomic approach to uncover novel biomarkers in fetal blood that correlate to neuropathology in the preterm sheep brain. The current data forms a basis for future studies to investigate mechanisms of these mRNAs in the injury progression.<\/jats:p>\n          <\/jats:sec>\n          <jats:sec>\n            <jats:title>Impact<\/jats:title>\n            <jats:p>\n              <jats:list list-type=\"bullet\">\n                <jats:list-item>\n                  <jats:p>Trend analysis of genes following asphyxia reveal a group of genes associated with perinatal death and growth failure.<\/jats:p>\n                <\/jats:list-item>\n                <jats:list-item>\n                  <jats:p>Several pre-asphyxia transcripts were associated to brain injury severity suggesting genomic susceptibility to injury.<\/jats:p>\n                <\/jats:list-item>\n                <jats:list-item>\n                  <jats:p>Several post-asphyxia transcripts were correlated to brain injury severity, thus, serve as potential novel biomarkers of injury outcome.<\/jats:p>\n                <\/jats:list-item>\n                <jats:list-item>\n                  <jats:p>Successfully adaptation of cell profiling algorithms suggests significant changes in blood cell composition following asphyxia.<\/jats:p>\n                <\/jats:list-item>\n              <\/jats:list>\n            <\/jats:p>\n          <\/jats:sec>","DOI":"10.1038\/s41390-024-03224-1","type":"journal-article","created":{"date-parts":[[2024,5,31]],"date-time":"2024-05-31T14:04:23Z","timestamp":1717164263000},"page":"1707-1717","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Novel biomarkers of preterm brain injury from blood transcriptome in sheep model of intrauterine asphyxia"],"prefix":"10.1038","volume":"96","author":[{"given":"C. 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