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Although a significant progress has been made toward recognizing about the phenotypic characteristics of MSCs, the true identity and properties of MSCs in bone marrow remain unclear. Here, we report the expression landscape of human fetal BM nucleated cells (BMNCs) based on the single-cell transcriptomic analysis. Unexpectedly, while the common cell surface markers such as CD146, CD271, and PDGFRa used for isolating MSCs were not detected, LIFR\n                    <jats:sup>+<\/jats:sup>\n                    PDGFRB\n                    <jats:sup>+<\/jats:sup>\n                    were identified to be specific markers of MSCs as the early progenitors. In vivo transplantation demonstrated that LIFR\n                    <jats:sup>+<\/jats:sup>\n                    PDGFRB\n                    <jats:sup>+<\/jats:sup>\n                    CD45\n                    <jats:sup>-<\/jats:sup>\n                    CD31\n                    <jats:sup>-<\/jats:sup>\n                    CD235a\n                    <jats:sup>-<\/jats:sup>\n                    MSCs could form bone tissues and reconstitute the hematopoietic microenvironment (HME) effectively in vivo. Interestingly, we also identified a subpopulation of bone unipotent progenitor expressing TM4SF1\n                    <jats:sup>+<\/jats:sup>\n                    CD44\n                    <jats:sup>+<\/jats:sup>\n                    CD73\n                    <jats:sup>+<\/jats:sup>\n                    CD45\n                    <jats:sup>-<\/jats:sup>\n                    CD31\n                    <jats:sup>-<\/jats:sup>\n                    CD235a\n                    <jats:sup>-<\/jats:sup>\n                    , which had osteogenic potentials, but could not reconstitute HME. MSCs expressed a set of different transcription factors at the different stages of human fetal bone marrow, indicating that the stemness properties of MSCs might change during development. Moreover, transcriptional characteristics of cultured MSCs were significantly changed compared with freshly isolated primary MSCs. Our cellular profiling provides a general landscape of heterogeneity, development, hierarchy, microenvironment of the human fetal BM-derived stem cells at single-cell resolution.\n                  <\/jats:p>","DOI":"10.1038\/s41392-023-01338-2","type":"journal-article","created":{"date-parts":[[2023,3,30]],"date-time":"2023-03-30T21:02:34Z","timestamp":1680210154000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":47,"title":["Characterization of mesenchymal stem cells in human fetal bone marrow by single-cell transcriptomic and functional analysis"],"prefix":"10.1038","volume":"8","author":[{"given":"Ping","family":"Zhang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8953-5284","authenticated-orcid":false,"given":"Ji","family":"Dong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoying","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Yong","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Yang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunsong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longwei","family":"Lv","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lu","family":"Wen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jie","family":"Qiao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuchou","family":"Tang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4332-0878","authenticated-orcid":false,"given":"Yongsheng","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2023,3,31]]},"reference":[{"key":"1338_CR1","doi-asserted-by":"publisher","first-page":"dev179325","DOI":"10.1242\/dev.179325","volume":"147","author":"MA Serowoky","year":"2020","unstructured":"Serowoky, M. 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