{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T00:03:16Z","timestamp":1793318596598,"version":"4.5.0"},"reference-count":86,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T00:00:00Z","timestamp":1580083200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"},{"start":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T00:00:00Z","timestamp":1580083200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T00:00:00Z","timestamp":1580083200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"funder":[{"DOI":"10.13039\/100000072","name":"U.S. Department of Health & Human Services | NIH | National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE027999"],"award-info":[{"award-number":["DE027999"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"U.S. Department of Health & Human Services | NIH | National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE018893"],"award-info":[{"award-number":["DE018893"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"U.S. Department of Health & Human Services | NIH | National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE022083"],"award-info":[{"award-number":["DE022083"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"U.S. Department of Health & Human Services | NIH | National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE021726"],"award-info":[{"award-number":["DE021726"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"U.S. Department of Health & Human Services | NIH | National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE027999"],"award-info":[{"award-number":["DE027999"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"U.S. Department of Health & Human Services | NIH | National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE018893"],"award-info":[{"award-number":["DE018893"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"U.S. Department of Health & Human Services | NIH | National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE022083"],"award-info":[{"award-number":["DE022083"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000072","name":"U.S. Department of Health & Human Services | NIH | National Institute of Dental and Craniofacial Research","doi-asserted-by":"publisher","award":["DE021726"],"award-info":[{"award-number":["DE021726"]}],"id":[{"id":"10.13039\/100000072","id-type":"DOI","asserted-by":"publisher"}]},{"name":"U.S. Department of Health & Human Services | NIH | National Institute of Dental and Craniofacial Research"},{"name":"U.S. Department of Health & Human Services | NIH | National Institute of Dental and Craniofacial Research"},{"name":"U.S. Department of Health & Human Services | NIH | National Institute of Dental and Craniofacial Research"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,5,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Many commensal oral streptococci generate H2O2 via pyruvate oxidase (SpxB) to inhibit the growth of competing bacteria like Streptococcus mutans, a major cariogenic species. In Streptococcus sanguinis SK36 (SK36) and Streptococcus gordonii DL1 (DL1), spxB expression and H2O2 release are subject to carbon catabolite repression by the catabolite control protein A (CcpA). Surprisingly, ccpA deletion mutants of SK36 and DL1 fail to inhibit S. mutans despite their production of otherwise inhibitory levels of H2O2. Using H2O2-deficient spxB deletion mutants of SK36 and DL1, it was subsequently discovered that both strains confer protection in trans to other bacteria when H2O2 is added exogenously. This protective effect depends on the direct detoxification of H2O2 by the release of pyruvate. The pyruvate dependent protective effect is also present in other spxB-encoding streptococci, such as the pneumococcus, but is missing from spxB-negative species like S. mutans. Targeted and transposon-based mutagenesis revealed Nox (putative H2O-forming NADH dehydrogenase) as an essential component required for pyruvate release and oxidative protection, while other genes such as sodA and dps play minor roles. Furthermore, pyruvate secretion is only detectable in aerobic growth conditions at biofilm-like cell densities and is responsive to CcpA-dependent catabolite control. This ability of spxB-encoding streptococci reveals a new facet of the competitive interactions between oral commensals and pathobionts and provides a mechanistic basis for the variable levels of inhibitory potential observed among H2O2-producing strains of commensal oral streptococci.<\/jats:p>","DOI":"10.1038\/s41396-020-0592-8","type":"journal-article","created":{"date-parts":[[2020,1,27]],"date-time":"2020-01-27T13:03:41Z","timestamp":1580130221000},"page":"1074-1088","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":58,"title":["Pyruvate secretion by oral streptococci modulates hydrogen peroxide dependent antagonism"],"prefix":"10.1093","volume":"14","author":[{"given":"Sylvio","family":"Redanz","sequence":"first","affiliation":[{"name":"Department of Restorative Dentistry, Oregon Health & Science University , Portland, OR 97239, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Puthayalai","family":"Treerat","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, Oregon Health & Science University , Portland, OR 97239, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rong","family":"Mu","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, Oregon Health & Science University , Portland, OR 97239, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrike","family":"Redanz","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, Oregon Health & Science University , Portland, OR 97239, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhengzhong","family":"Zou","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, Oregon Health & Science University , Portland, OR 97239, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dipankar","family":"Koley","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Oregon State University , Corvallis, OR 97331, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3464-0490","authenticated-orcid":false,"given":"Justin","family":"Merritt","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, Oregon Health & Science University , Portland, OR 97239, USA"},{"name":"Department of Molecular Microbiology and Immunology, School of Medicine, Oregon Health & Science University , Portland, OR 97239, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8599-8310","authenticated-orcid":false,"given":"Jens","family":"Kreth","sequence":"additional","affiliation":[{"name":"Department of Restorative Dentistry, Oregon Health & Science University , Portland, OR 97239, USA"},{"name":"Department of Molecular Microbiology and Immunology, School of Medicine, Oregon Health & Science University , Portland, OR 97239, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,1,27]]},"reference":[{"key":"2024011300300077800_CR1","doi-asserted-by":"crossref","first-page":"5002","DOI":"10.1128\/JB.00542-10","article-title":"The human oral microbiome","volume":"192","author":"Dewhirst","year":"2010","journal-title":"J Bacteriol"},{"key":"2024011300300077800_CR2","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1111\/j.1574-6968.2004.tb09643.x","article-title":"What drives bacteria to produce a biofilm?","volume":"236","author":"Jefferson","year":"2004","journal-title":"FEMS Microbiol Lett"},{"key":"2024011300300077800_CR3","doi-asserted-by":"crossref","first-page":"435","DOI":"10.4161\/viru.2.5.16140","article-title":"Bacterial interactions in dental biofilm","volume":"2","author":"Huang","year":"2011","journal-title":"Virulence."},{"key":"2024011300300077800_CR4","doi-asserted-by":"crossref","first-page":"40","DOI":"10.1034\/j.1600-0757.2002.03004.x","article-title":"Beneficial bacteria of the periodontium","volume":"30","author":"Roberts","year":"2002","journal-title":"Periodontology 2000"},{"key":"2024011300300077800_CR5","doi-asserted-by":"crossref","first-page":"405","DOI":"10.1089\/dna.2009.0874","article-title":"The oral microbiota: living with a permanent guest","volume":"28","author":"Avila","year":"2009","journal-title":"DNA Cell Biol"},{"key":"2024011300300077800_CR6","first-page":"43","article-title":"Bacteriology of dental infections","volume":"14","author":"Asikainen","year":"1993","journal-title":"Eur Heart J"},{"key":"2024011300300077800_CR7","doi-asserted-by":"crossref","first-page":"547","DOI":"10.1128\/CMR.13.4.547","article-title":"Systemic diseases caused by oral infection","volume":"13","author":"Li","year":"2000","journal-title":"Clin Microbiol Rev"},{"key":"2024011300300077800_CR8","doi-asserted-by":"crossref","first-page":"1599","DOI":"10.1016\/S1286-4579(00)01316-2","article-title":"Dental plaque formation","volume":"2","author":"Rosan","year":"2000","journal-title":"Microbes Infect"},{"key":"2024011300300077800_CR9","doi-asserted-by":"crossref","first-page":"13","DOI":"10.1099\/mic.0.039669-0","article-title":"Identification of hydrogen peroxide production-related genes in Streptococcus sanguinis and their functional relationship with pyruvate oxidase","volume":"157","author":"Chen","year":"2011","journal-title":"Microbiology"},{"key":"2024011300300077800_CR10","doi-asserted-by":"crossref","first-page":"e00158","DOI":"10.1128\/mSystems.00158-18","article-title":"Species designations belie phenotypic and genotypic heterogeneity in oral streptococci","volume":"3","author":"Velsko","year":"2018","journal-title":"mSystems"},{"key":"2024011300300077800_CR11","doi-asserted-by":"crossref","first-page":"337","DOI":"10.1111\/omi.12231","article-title":"Live and let die: hydrogen peroxide production by the commensal flora and its role in maintaining a symbiotic microbiome","volume":"33","author":"Redanz","year":"2018","journal-title":"Mol Oral Microbiol"},{"key":"2024011300300077800_CR12","doi-asserted-by":"crossref","first-page":"717843","DOI":"10.1155\/2012\/717843","article-title":"The role of hydrogen peroxide in environmental adaptation of oral microbial communities","volume":"2012","author":"Zhu","year":"2012","journal-title":"Oxid Med Cell Longev"},{"key":"2024011300300077800_CR13","doi-asserted-by":"crossref","first-page":"82","DOI":"10.4248\/IJOS11030","article-title":"Oxygen dependent pyruvate oxidase expression and production in Streptococcus sanguinis","volume":"3","author":"Zheng","year":"2011","journal-title":"Int J Oral Sci"},{"key":"2024011300300077800_CR14","doi-asserted-by":"crossref","first-page":"1436","DOI":"10.1128\/AEM.03306-12","article-title":"dpr and sod in Streptococcus mutans are involved in coexistence with S. sanguinis, and PerR is associated with resistance to H2O2","volume":"79","author":"Fujishima","year":"2013","journal-title":"Appl Environ Microbiol"},{"key":"2024011300300077800_CR15","doi-asserted-by":"crossref","first-page":"6815","DOI":"10.1128\/JB.185.23.6815-6825.2003","article-title":"Factors contributing to hydrogen peroxide resistance in Streptococcus pneumoniae include pyruvate oxidase (SpxB) and avoidance of the toxic effects of the fenton reaction","volume":"185","author":"Pericone","year":"2003","journal-title":"J Bacteriol"},{"key":"2024011300300077800_CR16","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1111\/j.1365-2958.2011.07603.x","article-title":"Mechanism of adhesion maintenance by methionine sulphoxide reductase in Streptococcus gordonii","volume":"80","author":"Lei","year":"2011","journal-title":"Mol Microbiol"},{"key":"2024011300300077800_CR17","doi-asserted-by":"crossref","first-page":"2627","DOI":"10.1099\/mic.0.082156-0","article-title":"Comparison of genes required for H2O2 resistance in Streptococcus gordonii and Streptococcus sanguinis","volume":"160","author":"Xu","year":"2014","journal-title":"Microbiology."},{"key":"2024011300300077800_CR18","doi-asserted-by":"crossref","first-page":"3166","DOI":"10.1128\/JB.01808-06","article-title":"Genome of the opportunistic pathogen Streptococcus sanguinis","volume":"189","author":"Xu","year":"2007","journal-title":"J Bacteriol"},{"key":"2024011300300077800_CR19","doi-asserted-by":"crossref","first-page":"63","DOI":"10.1111\/j.1574-6968.1996.tb08557.x","article-title":"Impaired oxidative stress resistance of Bacillus subtilis sigB mutants and the role of katA and katE","volume":"145","author":"Engelmann","year":"1996","journal-title":"FEMS Microbiol Lett"},{"key":"2024011300300077800_CR20","doi-asserted-by":"crossref","first-page":"4632","DOI":"10.1128\/JB.00276-08","article-title":"Streptococcal antagonism in oral biofilms: Streptococcus sanguinis and Streptococcus gordonii interference with Streptococcus mutans","volume":"190","author":"Kreth","year":"2008","journal-title":"J Bacteriol"},{"key":"2024011300300077800_CR21","doi-asserted-by":"crossref","first-page":"7193","DOI":"10.1128\/JB.187.21.7193-7203.2005","article-title":"Competition and coexistence between Streptococcus mutans and Streptococcus sanguinis in the dental biofilm","volume":"187","author":"Kreth","year":"2005","journal-title":"J Bacteriol"},{"key":"2024011300300077800_CR22","doi-asserted-by":"crossref","first-page":"436","DOI":"10.1099\/mic.0.066134-0","article-title":"Streptococcus mutans: a new Gram-positive paradigm?","volume":"159","author":"Lemos","year":"2013","journal-title":"Microbiology."},{"key":"2024011300300077800_CR23","doi-asserted-by":"crossref","first-page":"499","DOI":"10.1007\/s10096-013-1993-7","article-title":"The virulence of Streptococcus mutans and the ability to form biofilms","volume":"33","author":"Krzysciak","year":"2014","journal-title":"Eur J Clin Microbiol Infect Dis"},{"key":"2024011300300077800_CR24","doi-asserted-by":"crossref","first-page":"516","DOI":"10.1128\/JB.01131-10","article-title":"Catabolite control protein A controls hydrogen peroxide production and cell death in Streptococcus sanguinis","volume":"193","author":"Zheng","year":"2011","journal-title":"J Bacteriol"},{"key":"2024011300300077800_CR25","doi-asserted-by":"crossref","first-page":"4318","DOI":"10.1128\/AEM.00309-11","article-title":"Environmental influences on competitive hydrogen peroxide production in Streptococcus gordonii","volume":"77","author":"Zheng","year":"2011","journal-title":"Appl Environ Microbiol"},{"key":"2024011300300077800_CR26","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1093\/femsre\/fuv015","article-title":"Giving structure to the biofilm matrix: an overview of individual strategies and emerging common themes","volume":"39","author":"Hobley","year":"2015","journal-title":"FEMS Microbiol Rev"},{"key":"2024011300300077800_CR27","volume-title":"Molecular cloning: a laboratory manual","author":"Sambrook","year":"1989","edition":"2nd"},{"key":"2024011300300077800_CR28","doi-asserted-by":"crossref","first-page":"117","DOI":"10.1111\/j.2041-1014.2010.00602.x","article-title":"Characterization of competence and biofilm development of a Streptococcus sanguinis endocarditis isolate","volume":"26","author":"Zhu","year":"2011","journal-title":"Mol Oral Microbiol"},{"key":"2024011300300077800_CR29","doi-asserted-by":"crossref","first-page":"e01697","DOI":"10.1128\/AEM.01697-17","article-title":"Plasticity of the pyruvate node modulates hydrogen peroxide production and acid tolerance in multiple oral streptococci","volume":"84","author":"Cheng","year":"2018","journal-title":"Appl Environ Microbiol"},{"key":"2024011300300077800_CR30","doi-asserted-by":"crossref","first-page":"36111","DOI":"10.1074\/jbc.M112.379230","article-title":"Heterologous expression of sahH reveals that biofilm formation is autoinducer-2-independent in Streptococcus sanguinis but is associated with an intact activated methionine cycle","volume":"287","author":"Redanz","year":"2012","journal-title":"J Biol Chem"},{"key":"2024011300300077800_CR31","first-page":"42","volume-title":"Molecular biology of streptococci","author":"Johnsborg","year":"2007"},{"key":"2024011300300077800_CR32","doi-asserted-by":"crossref","first-page":"889","DOI":"10.1111\/j.1348-0421.2007.tb03971.x","article-title":"A novel agar medium to detect hydrogen peroxide-producing bacteria based on the prussian blue-forming reaction","volume":"51","author":"Saito","year":"2007","journal-title":"Microbiol Immunol"},{"key":"2024011300300077800_CR33","doi-asserted-by":"crossref","first-page":"e00619","DOI":"10.1128\/JB.00619-17","article-title":"Distinct regulatory role of carbon catabolite protein A (CcpA) in oral StreptococcalspxB expression","volume":"200","author":"Redanz","year":"2018","journal-title":"J Bacteriol"},{"key":"2024011300300077800_CR34","doi-asserted-by":"crossref","first-page":"1E 3 1","DOI":"10.1002\/9780471729259.mc01e03s36","article-title":"Genome-wide fitness and genetic interactions determined by Tn-seq, a high-throughput massively parallel sequencing method for microorganisms","volume":"36","author":"van Opijnen","year":"2015","journal-title":"Curr Protoc Microbiol"},{"key":"2024011300300077800_CR35","doi-asserted-by":"crossref","first-page":"e122","DOI":"10.1093\/nar\/gni124","article-title":"SiteFinding-PCR: a simple and efficient PCR method for chromosome walking","volume":"33","author":"Tan","year":"2005","journal-title":"Nucleic Acids Res"},{"key":"2024011300300077800_CR36","doi-asserted-by":"crossref","first-page":"146","DOI":"10.1016\/j.ab.2009.09.017","article-title":"A sensitive fluorimetric assay for pyruvate","volume":"396","author":"Zhu","year":"2010","journal-title":"Anal Biochem"},{"key":"2024011300300077800_CR37","doi-asserted-by":"crossref","first-page":"736837","DOI":"10.1155\/2012\/736837","article-title":"Glutathione homeostasis and functions: potential targets for medical interventions","volume":"2012","author":"Lushchak","year":"2012","journal-title":"J Amino Acids"},{"key":"2024011300300077800_CR38","doi-asserted-by":"crossref","first-page":"819","DOI":"10.1177\/0022034513498598","article-title":"Involvement of gshAB in the interspecies competition within oral biofilm","volume":"92","author":"Zheng","year":"2013","journal-title":"J Dent Res"},{"key":"2024011300300077800_CR39","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1128\/jb.168.1.109-114.1986","article-title":"Export of glutathione by some widely used Salmonella typhimurium and Escherichia coli strains","volume":"168","author":"Owens","year":"1986","journal-title":"J Bacteriol"},{"key":"2024011300300077800_CR40","doi-asserted-by":"crossref","first-page":"2351","DOI":"10.1128\/AEM.00064-14","article-title":"PerR-regulated manganese ion uptake contributes to oxidative stress defense in an oral streptococcus","volume":"80","author":"Wang","year":"2014","journal-title":"Appl Environ Microbiol"},{"key":"2024011300300077800_CR41","doi-asserted-by":"crossref","first-page":"745","DOI":"10.1186\/1471-2164-14-745","article-title":"RegPrecise 3.0 - a resource for genome-scale exploration of transcriptional regulation in bacteria","volume":"14","author":"Novichkov","year":"2013","journal-title":"BMC Genom"},{"key":"2024011300300077800_CR42","doi-asserted-by":"crossref","first-page":"125","DOI":"10.1038\/srep00125","article-title":"Genome-wide essential gene identification in Streptococcus sanguinis","volume":"1","author":"Xu","year":"2011","journal-title":"Sci Rep."},{"key":"2024011300300077800_CR43","doi-asserted-by":"crossref","first-page":"375","DOI":"10.1111\/j.1365-2672.2010.04890.x","article-title":"Structure, function and regulation of the DNA-binding protein Dps and its role in acid and oxidative stress resistance in Escherichia coli: a review","volume":"110","author":"Calhoun","year":"2011","journal-title":"J Appl Microbiol"},{"key":"2024011300300077800_CR44","doi-asserted-by":"crossref","first-page":"104","DOI":"10.1038\/s41586-019-1151-1","article-title":"Synthesis and breakdown of universal metabolic precursors promoted by iron","volume":"569","author":"Muchowska","year":"2019","journal-title":"Nature."},{"key":"2024011300300077800_CR45","doi-asserted-by":"crossref","first-page":"1470","DOI":"10.1128\/IAI.01203-15","article-title":"Involvement of NADH oxidase in competition and endocarditis virulence in Streptococcus sanguinis","volume":"84","author":"Ge","year":"2016","journal-title":"Infect Immun"},{"key":"2024011300300077800_CR46","doi-asserted-by":"crossref","first-page":"e68277","DOI":"10.1371\/journal.pone.0068277","article-title":"Pyruvate oxidase influences the sugar utilization pattern and capsule production in Streptococcus pneumoniae","volume":"8","author":"Carvalho","year":"2013","journal-title":"PloS one"},{"key":"2024011300300077800_CR47","doi-asserted-by":"crossref","first-page":"W604","DOI":"10.1093\/nar\/gks562","article-title":"RegPrecise web services interface: programmatic access to the transcriptional regulatory interactions in bacteria reconstructed by comparative genomics","volume":"40","author":"Novichkov","year":"2012","journal-title":"Nucleic Acids Res"},{"key":"2024011300300077800_CR48","doi-asserted-by":"crossref","first-page":"D111","DOI":"10.1093\/nar\/gkp894","article-title":"RegPrecise: a database of curated genomic inferences of transcriptional regulatory interactions in prokaryotes","volume":"38","author":"Novichkov","year":"2010","journal-title":"Nucleic Acids Res"},{"key":"2024011300300077800_CR49","doi-asserted-by":"crossref","first-page":"5940","DOI":"10.1128\/JB.181.19.5940-5947.1999","article-title":"Functions of two types of NADH oxidases in energy metabolism and oxidative stress of Streptococcus mutans","volume":"181","author":"Higuchi","year":"1999","journal-title":"J Bacteriol"},{"key":"2024011300300077800_CR50","doi-asserted-by":"crossref","first-page":"e58492","DOI":"10.1371\/journal.pone.0058492","article-title":"Environmental and nutritional factors that affect growth and metabolism of the pneumococcal serotype 2 strain D39 and its nonencapsulated derivative strain R6","volume":"8","author":"Carvalho","year":"2013","journal-title":"PLoS One"},{"key":"2024011300300077800_CR51","doi-asserted-by":"crossref","first-page":"1300","DOI":"10.3389\/fmicb.2018.01300","article-title":"NADH-Mediated Gene Expression in Streptococcus pneumoniae and Role of Rex as a Transcriptional Repressor of the Rex-Regulon","volume":"9","author":"Afzal","year":"2018","journal-title":"Front Microbiol"},{"key":"2024011300300077800_CR52","doi-asserted-by":"crossref","first-page":"2166","DOI":"10.1128\/JB.01542-14","article-title":"Streptococcus mutans NADH oxidase lies at the intersection of overlapping regulons controlled by oxygen and NAD+ levels","volume":"196","author":"Baker","year":"2014","journal-title":"J Bacteriol"},{"key":"2024011300300077800_CR53","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1080\/21505594.2016.1201256","article-title":"Contribution of NADH oxidase to oxidative stress tolerance and virulence of Streptococcus suis serotype 2","volume":"8","author":"Zheng","year":"2017","journal-title":"Virulence."},{"key":"2024011300300077800_CR54","doi-asserted-by":"crossref","first-page":"4","DOI":"10.1128\/jb.88.1.4-10.1964","article-title":"Pyruvate fermentation by Streptococcus faecalis","volume":"88","author":"Deibel","year":"1964","journal-title":"J Bacteriol"},{"key":"2024011300300077800_CR55","doi-asserted-by":"crossref","first-page":"E1578","DOI":"10.1073\/pnas.1714645115","article-title":"Pyruvate cycle increases aminoglycoside efficacy and provides respiratory energy in bacteria","volume":"115","author":"Su","year":"2018","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024011300300077800_CR56","doi-asserted-by":"crossref","first-page":"4260","DOI":"10.1074\/jbc.M111.304311","article-title":"Characterization of central carbon metabolism of Streptococcus pneumoniae by isotopologue profiling","volume":"287","author":"Hartel","year":"2012","journal-title":"J Biol Chem"},{"key":"2024011300300077800_CR57","doi-asserted-by":"crossref","first-page":"9060","DOI":"10.1523\/JNEUROSCI.17-23-09060.1997","article-title":"Pyruvate protects neurons against hydrogen peroxide-induced toxicity","volume":"17","author":"Desagher","year":"1997","journal-title":"J Neurosci"},{"key":"2024011300300077800_CR58","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.brainres.2006.11.032","article-title":"Pyruvate protects mitochondria from oxidative stress in human neuroblastoma SK-N-SH cells","volume":"1132","author":"Wang","year":"2007","journal-title":"Brain Res"},{"key":"2024011300300077800_CR59","doi-asserted-by":"crossref","first-page":"700","DOI":"10.1016\/j.bbrc.2009.08.069","article-title":"Artefacts in cell culture: pyruvate as a scavenger of hydrogen peroxide generated by ascorbate or epigallocatechin gallate in cell culture media","volume":"388","author":"Long","year":"2009","journal-title":"Biochem Biophys Res Commun"},{"key":"2024011300300077800_CR60","doi-asserted-by":"crossref","first-page":"4350","DOI":"10.1128\/AEM.68.9.4350-4356.2002","article-title":"Formation and conversion of oxygen metabolites by Lactococcus lactis subsp. lactis ATCC 19435 under different growth conditions","volume":"68","author":"van Niel","year":"2002","journal-title":"Appl Environ Microbiol"},{"key":"2024011300300077800_CR61","doi-asserted-by":"crossref","first-page":"7888","DOI":"10.1073\/pnas.1605501113","article-title":"Hydrogen peroxide detoxification is a key mechanism for growth of ammonia-oxidizing archaea","volume":"113","author":"Kim","year":"2016","journal-title":"Proc Natl Acad Sci USA"},{"key":"2024011300300077800_CR62","doi-asserted-by":"crossref","first-page":"e84625","DOI":"10.1371\/journal.pone.0084625","article-title":"Pyruvate protects pathogenic spirochetes from H2O2 killing","volume":"9","author":"Troxell","year":"2014","journal-title":"PloS One"},{"key":"2024011300300077800_CR63","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.abb.2012.04.014","article-title":"Why do bacteria use so many enzymes to scavenge hydrogen peroxide?","volume":"525","author":"Mishra","year":"2012","journal-title":"Arch Biochem Biophys"},{"key":"2024011300300077800_CR64","doi-asserted-by":"crossref","first-page":"271","DOI":"10.1186\/s12866-016-0881-6","article-title":"Pyruvate oxidase of Streptococcus pneumoniae contributes to pneumolysin release","volume":"16","author":"Bryant","year":"2016","journal-title":"BMC Microbiol"},{"key":"2024011300300077800_CR65","doi-asserted-by":"crossref","first-page":"3990","DOI":"10.1128\/IAI.68.7.3990-3997.2000","article-title":"Inhibitory and bactericidal effects of hydrogen peroxide production by Streptococcus pneumoniae on other inhabitants of the upper respiratory tract","volume":"68","author":"Pericone","year":"2000","journal-title":"Infect Immun"},{"key":"2024011300300077800_CR66","doi-asserted-by":"crossref","first-page":"803","DOI":"10.1046\/j.1365-2958.1996.425954.x","article-title":"Pyruvate oxidase, as a determinant of virulence in Streptococcus pneumoniae","volume":"19","author":"Spellerberg","year":"1996","journal-title":"Mol Microbiol"},{"key":"2024011300300077800_CR67","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1128\/jb.169.1.380-385.1987","article-title":"Characterization of the specific pyruvate transport system in Escherichia coli K-12","volume":"169","author":"Lang","year":"1987","journal-title":"J Bacteriol"},{"key":"2024011300300077800_CR68","doi-asserted-by":"crossref","first-page":"e00599","DOI":"10.1128\/JB.00599-17","article-title":"BtsT, a novel and specific pyruvate\/H(+) symporter in Escherichia coli","volume":"200","author":"Kristoficova","year":"2018","journal-title":"J Bacteriol"},{"key":"2024011300300077800_CR69","doi-asserted-by":"crossref","first-page":"e00771","DOI":"10.1128\/JB.00771-17","article-title":"Peptide transporter CstA imports pyruvate in Escherichia coli K-12","volume":"200","author":"Hwang","year":"2018","journal-title":"J Bacteriol"},{"key":"2024011300300077800_CR70","doi-asserted-by":"crossref","first-page":"443","DOI":"10.1042\/BJ20141171","article-title":"Mitochondrial pyruvate transport: a historical perspective and future research directions","volume":"466","author":"McCommis","year":"2015","journal-title":"Biochem J"},{"key":"2024011300300077800_CR71","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1128\/JB.01155-08","article-title":"Identification and characterization of a bacterial transport system for the uptake of pyruvate, propionate, and acetate in Corynebacterium glutamicum","volume":"191","author":"Jolkver","year":"2009","journal-title":"J Bacteriol"},{"key":"2024011300300077800_CR72","doi-asserted-by":"crossref","first-page":"e00976","DOI":"10.1128\/mBio.00976-17","article-title":"Molecular and physiological logics of the pyruvate-induced response of a novel transporter in Bacillus subtilis","volume":"8","author":"Charbonnier","year":"2017","journal-title":"mBio"},{"key":"2024011300300077800_CR73","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1016\/j.mib.2011.12.010","article-title":"The control of death and lysis in staphylococcal biofilms: a coordination of physiological signals","volume":"15","author":"Sadykov","year":"2012","journal-title":"Curr Opin Microbiol"},{"key":"2024011300300077800_CR74","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1186\/s12866-019-1600-x","article-title":"Characterization of LrgAB as a stationary phase-specific pyruvate uptake system in Streptococcus mutans","volume":"19","author":"Ahn","year":"2019","journal-title":"BMC Microbiol"},{"key":"2024011300300077800_CR75","doi-asserted-by":"crossref","first-page":"969","DOI":"10.1016\/0378-4274(95)03532-X","article-title":"Toxicity of iron and hydrogen peroxide: the Fenton reaction","volume":"82-83","author":"Winterbourn","year":"1995","journal-title":"Toxicol Lett"},{"key":"2024011300300077800_CR76","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.mib.2008.02.007","article-title":"The mechanisms of carbon catabolite repression in bacteria","volume":"11","author":"Deutscher","year":"2008","journal-title":"Curr Opin Microbiol"},{"key":"2024011300300077800_CR77","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1093\/jac\/dkg423","article-title":"Antimicrobial susceptibility patterns and macrolide resistance genes of viridans group streptococci from normal flora","volume":"52","author":"Seppala","year":"2003","journal-title":"J Antimicrob Chemother"},{"key":"2024011300300077800_CR78","doi-asserted-by":"crossref","first-page":"425","DOI":"10.1146\/annurev-micro-091014-104209","article-title":"Mechanisms of bacterial colonization of the respiratory tract","volume":"69","author":"Siegel","year":"2015","journal-title":"Annu Rev Microbiol"},{"key":"2024011300300077800_CR79","doi-asserted-by":"crossref","first-page":"882","DOI":"10.1016\/S0140-6736(15)00067-7","article-title":"Infective endocarditis","volume":"387","author":"Cahill","year":"2016","journal-title":"Lancet."},{"key":"2024011300300077800_CR80","doi-asserted-by":"crossref","first-page":"794","DOI":"10.1099\/jmm.0.054213-0","article-title":"Streptococcus sanguinis meningitis following endoscopic ligation for oesophageal variceal haemorrhage","volume":"62","author":"Liu","year":"2013","journal-title":"J Med Microbiol"},{"key":"2024011300300077800_CR81","doi-asserted-by":"crossref","first-page":"893","DOI":"10.1016\/S0140-6736(09)61204-6","article-title":"Burden of disease caused by Streptococcus pneumoniae in children younger than 5 years: global estimates","volume":"374","author":"O\u2019Brien","year":"2009","journal-title":"Lancet."},{"key":"2024011300300077800_CR82","doi-asserted-by":"crossref","first-page":"541471","DOI":"10.1155\/2012\/541471","article-title":"Hydrogen peroxide in inflammation: messenger, guide, and assassin","volume":"2012","author":"Wittmann","year":"2012","journal-title":"Adv Hematol"},{"key":"2024011300300077800_CR83","doi-asserted-by":"crossref","first-page":"e213","DOI":"10.1038\/cddis.2011.96","article-title":"Hydrogen peroxide: a Jekyll and Hyde signalling molecule","volume":"2","author":"Gough","year":"2011","journal-title":"Cell Death Dis"},{"key":"2024011300300077800_CR84","doi-asserted-by":"crossref","first-page":"E156","DOI":"10.3390\/cells7100156","article-title":"The role of hydrogen peroxide in redox-dependent signaling: homeostatic and pathological responses in mammalian cells","volume":"7","author":"Di","year":"2018","journal-title":"Cells"},{"key":"2024011300300077800_CR85","doi-asserted-by":"crossref","first-page":"135","DOI":"10.1007\/s11010-018-3380-2","article-title":"Hydrogen peroxide-induced oxidative stress and its impact on innate immune responses in lung carcinoma A549 cells","volume":"450","author":"Upadhyay","year":"2019","journal-title":"Mol Cell Biochem"},{"key":"2024011300300077800_CR86","doi-asserted-by":"crossref","first-page":"2668","DOI":"10.1073\/pnas.1018391108","article-title":"Real-time mapping of a hydrogen peroxide concentration profile across a polymicrobial bacterial biofilm using scanning electrochemical microscopy","volume":"108","author":"Liu","year":"2011","journal-title":"Proc Natl Acad Sci USA"}],"container-title":["The ISME Journal"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/s41396-020-0592-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/s41396-020-0592-8","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/academic.oup.com\/ismej\/article-pdf\/14\/5\/1074\/55526332\/41396_2020_article_592.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/www.nature.com\/articles\/s41396-020-0592-8.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"},{"URL":"https:\/\/academic.oup.com\/ismej\/article-pdf\/14\/5\/1074\/55526332\/41396_2020_article_592.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,30]],"date-time":"2024-07-30T15:24:03Z","timestamp":1722353043000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/ismej\/article\/14\/5\/1074-1088\/7475020"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,27]]},"references-count":86,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2020,5,1]]}},"URL":"https:\/\/doi.org\/10.1038\/s41396-020-0592-8","relation":{},"ISSN":["1751-7362","1751-7370"],"issn-type":[{"value":"1751-7362","type":"print"},{"value":"1751-7370","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2020,5]]},"published":{"date-parts":[[2020,1,27]]},"assertion":[{"value":"2 August 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"14 January 2020","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"16 January 2020","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"27 January 2020","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}