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The etiopathogenesis of PD remains largely unknown, but both genetic and environmental factors contribute to risk. An epigenome-wide association study (EWAS) was conducted to compare medication-free PD patients (<jats:italic>n<\/jats:italic>\u2009=\u200989) with healthy controls (<jats:italic>n<\/jats:italic>\u2009=\u200976) stratified by gender. Replication was sought in an independent sample (131 cases, 169 controls) and functional analyses were conducted in a third sample (<jats:italic>N<\/jats:italic>\u2009=\u200971). DNA methylation was assessed in whole blood using the Infinium HumanMethylation450 BeadChip. One genome-wide association surviving FDR of 5% (cg07308824, <jats:italic>P<\/jats:italic>\u2009=\u20091.094\u2009\u00d7\u200910-7, P-adj\u2009=\u20090.046) was identified in female PD patients (<jats:italic>N<\/jats:italic>\u2009=\u200949) compared to controls (<jats:italic>N<\/jats:italic>\u2009=\u200948). The same locus, located in an enhancer region of the <jats:italic>HECA<\/jats:italic> gene, was also hypermethylated in female PD patients in the replication sample (<jats:italic>P<\/jats:italic>\u2009=\u20090.035) and the significance of the association improved in the meta-analysis (P-adj\u2009=\u20090.004). Methylation at this CpG site was associated with <jats:italic>HECA<\/jats:italic> mRNA expression in another independent female sample (<jats:italic>N<\/jats:italic>\u2009=\u200971) both at baseline (<jats:italic>P<\/jats:italic>\u2009=\u20090.046) and after induction by dexamethasone (<jats:italic>P<\/jats:italic>\u2009=\u20090.029). Of 15 candidates, 5 previously reported as associated with PD or anxiety traits also showed differences in DNA methylation after gene-wise correction and included <jats:italic>SGK1<\/jats:italic>, <jats:italic>FHIT<\/jats:italic>, <jats:italic>ADCYAP1<\/jats:italic>, <jats:italic>HTR1A<\/jats:italic>, <jats:italic>HTR2A<\/jats:italic>. Our study examines epigenome-wide differences in peripheral blood for PD patients. Our results point to possible sex-specific methylation changes in the <jats:italic>HECA<\/jats:italic> gene for PD but overall highlight that this disorder is not associated with extensive changes in DNA methylation in peripheral blood.<\/jats:p>","DOI":"10.1038\/s41398-017-0026-1","type":"journal-article","created":{"date-parts":[[2017,12,15]],"date-time":"2017-12-15T13:26:55Z","timestamp":1513344415000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":52,"title":["\u201cDNA Methylation signatures in panic disorder\u201d"],"prefix":"10.1038","volume":"7","author":[{"given":"Stella","family":"Iurato","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tania","family":"Carrillo-Roa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janine","family":"Arloth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Darina","family":"Czamara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Diener-H\u00f6lzl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Lange","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bertram","family":"M\u00fcller-Myhsok","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabeth B.","family":"Binder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelika","family":"Erhardt","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2017,12,18]]},"reference":[{"key":"26_CR1","doi-asserted-by":"crossref","unstructured":"American Psychiatric Association, Diagnostic and Statistical Manual of Mental Disorders (DSM-5\u00ae) (American Psychiatric Publishing, Arlington, VA, 2013).","DOI":"10.1176\/appi.books.9780890425596"},{"key":"26_CR2","doi-asserted-by":"publisher","first-page":"435","DOI":"10.1016\/j.euroneuro.2005.04.006","volume":"15","author":"RD Goodwin","year":"2005","unstructured":"Goodwin, R. 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