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Here, we conducted an epigenome-wide DNA methylation study employing 93 patients with GAD, 65 patients with OCD, and 302 health controls, to explore epigenetic alterations associated with the onset and differences of GAD and OCD. We identified multiple differentially methylated positions (DMPs) and regions (DMRs): three DMP genes included <jats:italic>RIOK3<\/jats:italic> (cg21515243, <jats:italic>p<\/jats:italic>\u2009=\u20098.00\u2009\u00d7\u200910<jats:sup>\u221210<\/jats:sup>), <jats:italic>DNASE2<\/jats:italic> (cg09379601, <jats:italic>p<\/jats:italic>\u2009=\u20091.10\u2009\u00d7\u200910<jats:sup>\u22129<\/jats:sup>), and <jats:italic>PSMB4<\/jats:italic> (cg01334186, <jats:italic>p<\/jats:italic>\u2009=\u20093.70\u2009\u00d7\u200910<jats:sup>\u22127<\/jats:sup>) and two DMR genes <jats:italic>USP6NL<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009=\u20094.50\u2009\u00d7\u200910<jats:sup>\u22124<\/jats:sup>) and <jats:italic>CPLX1<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009=\u20096.95\u2009\u00d7\u200910<jats:sup>\u22124<\/jats:sup>) were associated with the onset of GAD and OCD; three DMPs genes included <jats:italic>LDLRAP1<\/jats:italic> (cg21400344, <jats:italic>p<\/jats:italic>\u2009=\u20094.40\u2009\u00d7\u200910<jats:sup>\u221212<\/jats:sup>), <jats:italic>ACIN1<\/jats:italic> (cg23712970, <jats:italic>p<\/jats:italic>\u2009=\u20092.98\u00d710<jats:sup>\u221211<\/jats:sup>), and <jats:italic>SCRT1<\/jats:italic> (cg25472897, <jats:italic>p<\/jats:italic>\u2009=\u20095.60\u2009\u00d7\u200910<jats:sup>\u221211<\/jats:sup>) and three DMR genes <jats:italic>WDR19<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009=\u20093.39\u2009\u00d7\u200910<jats:sup>\u22123<\/jats:sup>), <jats:italic>SYCP1<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009=\u20096.41\u2009\u00d7\u200910<jats:sup>\u22123<\/jats:sup>), and <jats:italic>FAM172A<\/jats:italic> (<jats:italic>p<\/jats:italic>\u2009=\u20095.74\u2009\u00d7\u200910<jats:sup>\u22123<\/jats:sup>) were associated with the differences between GAD and OCD. Investigation of epigenetic age and chronological age revealed a different epigenetic development trajectory of GAD and OCD. Conclusively, our findings which yielded robust models may aid in distinguishing patients from healthy controls (AUC\u2009=\u20090.90\u20130.99) or classifying patients with GAD and OCD (AUC\u2009=\u20090.89\u20130.99), and may power the precision medicine for them.<\/jats:p>","DOI":"10.1038\/s41398-022-02236-x","type":"journal-article","created":{"date-parts":[[2022,11,7]],"date-time":"2022-11-07T16:06:57Z","timestamp":1667837217000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Epigenome-wide DNA methylation analysis of whole blood cells derived from patients with GAD and OCD in the Chinese Han population"],"prefix":"10.1038","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4223-7551","authenticated-orcid":false,"given":"Liangkun","family":"Guo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhaojun","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guiming","family":"Wei","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weiqiu","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuebing","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1201-8465","authenticated-orcid":false,"given":"Weihua","family":"Yue","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2022,11,7]]},"reference":[{"key":"2236_CR1","doi-asserted-by":"publisher","first-page":"1700","DOI":"10.1016\/S0140-6736(21)02143-7","volume":"398","author":"DF Santomauro","year":"2021","unstructured":"Santomauro DF, Herrera AMM, Shadid J, Zheng P, Ashbaugh C, Pigott DM, et al. 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