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Angiogenesis plays a pivotal role in the development and progression of malignant tumours. miR-143-3p acts as a tumour suppressor in various cancers. Their role in GBC is however less well defined. Here we show that the expression levels of miR-143-3p were decreased in human GBC tissues compared with the non-tumour adjacent tissue (NAT) counterparts and were closely associated with overall survival. We discovered that miR-143-3p was a novel inhibitor of tumour growth and angiogenesis in vivo and in vitro. Our antibody array, ELISA and <jats:italic>PLGF<\/jats:italic> rescue analyses indicated that <jats:italic>PLGF<\/jats:italic> played an essential role in the antiangiogenic effect of miR-143-3p. Furthermore, we used miRNA target-prediction software and dual-luciferase assays to confirm that integrin \u03b16 (<jats:italic>ITGA6<\/jats:italic>) acted as a direct target of miR-143-3p. Our ELISA and western blot analyses confirmed that the expression of <jats:italic>PLGF<\/jats:italic> was decreased via the <jats:italic>ITGA6\/PI3K\/AKT<\/jats:italic> pathway. In conclusion, miR-143-3p suppresses tumour angiogenesis and growth of GBC through the <jats:italic>ITGA6\/PI3K\/AKT\/PLGF<\/jats:italic> pathways and may be a novel molecular therapeutic target for GBC.<\/jats:p>","DOI":"10.1038\/s41419-017-0258-2","type":"journal-article","created":{"date-parts":[[2018,2,7]],"date-time":"2018-02-07T16:18:52Z","timestamp":1518020332000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":86,"title":["miR-143-3p targeting of ITGA6 suppresses tumour growth and angiogenesis by downregulating PLGF expression via the PI3K\/AKT pathway in gallbladder carcinoma"],"prefix":"10.1038","volume":"9","author":[{"given":"Yun-Peng","family":"Jin","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun-Ping","family":"Hu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang-Song","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao-Shi","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuan-Yuan","family":"Ye","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huai-Feng","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Chen","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lin","family":"Jiang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fa-Tao","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi-Jian","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya-Juan","family":"Hao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi-Yong","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying-Bin","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,2,7]]},"reference":[{"key":"258_CR1","doi-asserted-by":"publisher","first-page":"445","DOI":"10.1038\/cdd.2016.146","volume":"24","author":"Y Shu","year":"2017","unstructured":"Shu, Y. et al. 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