{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T02:57:49Z","timestamp":1793761069925,"version":"4.6.1"},"reference-count":54,"publisher":"Springer Science and Business Media LLC","issue":"1","license":[{"start":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T00:00:00Z","timestamp":1579651200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"},{"start":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T00:00:00Z","timestamp":1579651200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"funder":[{"DOI":"10.13039\/501100006313","name":"Barncancerfonden","doi-asserted-by":"publisher","award":["PR2018-0127"],"award-info":[{"award-number":["PR2018-0127"]}],"id":[{"id":"10.13039\/501100006313","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100006313","name":"Barncancerfonden","doi-asserted-by":"publisher","award":["TJ2017-0004"],"award-info":[{"award-number":["TJ2017-0004"]}],"id":[{"id":"10.13039\/501100006313","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002794","name":"Cancerfonden","doi-asserted-by":"publisher","award":["CAN 2016\/318"],"award-info":[{"award-number":["CAN 2016\/318"]}],"id":[{"id":"10.13039\/501100002794","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Vetenskapsr\u00e5det","doi-asserted-by":"publisher","award":["2015-03688"],"award-info":[{"award-number":["2015-03688"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Cell Death Dis"],"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Oncolytic viruses have the potential to induce immunogenic cell death (ICD) that may provoke potent and long-lasting anti-cancer immunity. Here we aimed to characterize the ICD-inducing ability of wild-type Adenovirus (Ad), Semliki Forest virus (SFV) and Vaccinia virus (VV). We did so by investigating the cell death and immune-activating properties of virus-killed tumor cells. Ad-infection of tumor cells primarily activates autophagy, but also activate events of necroptotic and pyroptotic cell death. SFV infection on the other hand primarily activates immunogenic apoptosis while VV activates necroptosis. All viruses mediated lysis of tumor cells leading to the release of danger-associated molecular patterns, triggering of phagocytosis and maturation of dendritic cells (DCs). However, only SFV-infected tumor cells triggered significant T helper type 1 (Th1)-cytokine release by DCs and induced antigen-specific T-cell activation. Our results elucidate cell death processes activated upon Ad, SFV, and VV infection and their potential to induce T cell-mediated anti-tumor immune responses. This knowledge provides important insight for the choice and design of therapeutically successful virus-based immunotherapies.<\/jats:p>","DOI":"10.1038\/s41419-020-2236-3","type":"journal-article","created":{"date-parts":[[2020,1,22]],"date-time":"2020-01-22T14:03:52Z","timestamp":1579701832000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":193,"title":["Characterization of virus-mediated immunogenic cancer cell death and the consequences for oncolytic virus-based immunotherapy of cancer"],"prefix":"10.1038","volume":"11","author":[{"given":"Jing","family":"Ma","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohanraj","family":"Ramachandran","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan","family":"Jin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clara","family":"Quijano-Rubio","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miika","family":"Martikainen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8636-0351","authenticated-orcid":false,"given":"Di","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magnus","family":"Essand","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,1,22]]},"reference":[{"key":"2236_CR1","doi-asserted-by":"publisher","first-page":"486","DOI":"10.1038\/s41418-017-0012-4","volume":"25","author":"L Galluzzi","year":"2018","unstructured":"Galluzzi, L. et al. Molecular mechanisms of cell death: recommendations of the Nomenclature Committee on Cell Death 2018. Cell Death Differ. 25, 486\u2013541 (2018).","journal-title":"Cell Death Differ."},{"key":"2236_CR2","first-page":"74","volume":"4","author":"ZS Guo","year":"2014","unstructured":"Guo, Z. S., Liu, Z. & Bartlett, D. L., Oncolytic Immunotherapy. Dying the right way is a key to eliciting potent antitumor immunity. Front. Oncol. 4, 74 (2014).","journal-title":"Front. Oncol."},{"key":"2236_CR3","doi-asserted-by":"publisher","first-page":"938","DOI":"10.1038\/cdd.2016.5","volume":"23","author":"AD Garg","year":"2016","unstructured":"Garg, A. D., Romano, E., Rufo, N. & Agostinis, P. Immunogenic versus tolerogenic phagocytosis during anticancer therapy: mechanisms and clinical translation. Cell Death Differ. 23, 938\u2013951 (2016).","journal-title":"Cell Death Differ."},{"key":"2236_CR4","doi-asserted-by":"publisher","first-page":"1691","DOI":"10.1084\/jem.20050915","volume":"202","author":"N Casares","year":"2005","unstructured":"Casares, N. et al. Caspase-dependent immunogenicity of doxorubicin-induced tumor cell death. J. Exp. Med. 202, 1691\u20131701 (2005).","journal-title":"J. Exp. Med."},{"key":"2236_CR5","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1146\/annurev-immunol-032712-100008","volume":"31","author":"G Kroemer","year":"2013","unstructured":"Kroemer, G., Galluzzi, L., Kepp, O. & Zitvogel, L. Immunogenic cell death in cancer therapy. Annu. Rev. Immunol. 31, 51\u201372 (2013).","journal-title":"Annu. Rev. Immunol."},{"key":"2236_CR6","doi-asserted-by":"publisher","DOI":"10.4161\/21624011.2014.955691","volume":"3","author":"O Kepp","year":"2014","unstructured":"Kepp, O. et al. Consensus guidelines for the detection of immunogenic cell death. Oncoimmunology 3, e955691 (2014).","journal-title":"Oncoimmunology"},{"key":"2236_CR7","doi-asserted-by":"publisher","first-page":"1109","DOI":"10.1016\/j.cell.2017.08.027","volume":"170","author":"A Ribas","year":"2017","unstructured":"Ribas, A. et al. Oncolytic virotherapy promotes intratumoral T cell infiltration and improves anti-PD-1 immunotherapy. Cell 170, 1109\u20131119 (2017). e1110.","journal-title":"Cell"},{"key":"2236_CR8","doi-asserted-by":"publisher","first-page":"922","DOI":"10.1158\/0008-5472.CAN-15-3308","volume":"78","author":"M Kim","year":"2018","unstructured":"Kim, M. et al. Amplification of oncolytic Vaccinia virus widespread tumor cell killing by sunitinib through multiple mechanisms. Cancer Res. 78, 922\u2013937 (2018).","journal-title":"Cancer Res."},{"key":"2236_CR9","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1111\/j.1365-2796.2010.02223.x","volume":"267","author":"L Galluzzi","year":"2010","unstructured":"Galluzzi, L. et al. Viral strategies for the evasion of immunogenic cell death. J. Intern. Med. 267, 526\u2013542 (2010).","journal-title":"J. Intern. Med."},{"key":"2236_CR10","doi-asserted-by":"publisher","first-page":"1048","DOI":"10.1158\/1078-0432.CCR-15-2667","volume":"22","author":"FJ Kohlhapp","year":"2016","unstructured":"Kohlhapp, F. J. & Kaufman, H. L. Molecular pathways: mechanism of action for talimogene laherparepvec, a new oncolytic virus immunotherapy. Clin. Cancer Res. 22, 1048\u20131054 (2016).","journal-title":"Clin. Cancer Res."},{"key":"2236_CR11","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1159\/000448430","volume":"105","author":"D Yu","year":"2016","unstructured":"Yu, D. et al. Preclinical evaluation of AdVince, an oncolytic adenovirus adapted for treatment of liver metastases from neuroendocrine cancer. Neuroendocrinology 105, 54\u201366 (2016).","journal-title":"Neuroendocrinology"},{"key":"2236_CR12","doi-asserted-by":"publisher","first-page":"642","DOI":"10.1038\/nrd4663","volume":"14","author":"HL Kaufman","year":"2015","unstructured":"Kaufman, H. L., Kohlhapp, F. J. & Zloza, A. Oncolytic viruses: a new class of immunotherapy drugs. Nat. Rev. Drug Discov. 14, 642\u2013662 (2015).","journal-title":"Nat. Rev. Drug Discov."},{"key":"2236_CR13","doi-asserted-by":"publisher","first-page":"631","DOI":"10.1189\/jlb.5RU0117-040R","volume":"102","author":"J De Munck","year":"2017","unstructured":"De Munck, J., Binks, A., McNeish, I. A. & Aerts, J. L. Oncolytic virus-induced cell death and immunity: a match made in heaven? J. Leukoc. Biol. 102, 631\u2013643 (2017).","journal-title":"J. Leukoc. Biol."},{"key":"2236_CR14","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1159\/000448430","volume":"105","author":"D Yu","year":"2017","unstructured":"Yu, D. et al. Preclinical evaluation of AdVince, an oncolytic adenovirus adapted for treatment of liver metastases from neuroendocrine cancer. Neuroendocrinology 105, 54\u201366 (2017).","journal-title":"Neuroendocrinology"},{"key":"2236_CR15","doi-asserted-by":"publisher","first-page":"1519","DOI":"10.1158\/1078-0432.CCR-16-0925","volume":"23","author":"M Ramachandran","year":"2017","unstructured":"Ramachandran, M. et al. Safe and effective treatment of experimental neuroblastoma and glioblastoma using systemically delivered triple MicroRNA-detargeted oncolytic Semliki Forest virus. Clin. Cancer Res. 23, 1519\u20131530 (2017).","journal-title":"Clin. Cancer Res."},{"key":"2236_CR16","doi-asserted-by":"publisher","first-page":"10637","DOI":"10.1128\/JVI.01868-15","volume":"89","author":"M Martikainen","year":"2015","unstructured":"Martikainen, M. et al. MicroRNA-attenuated clone of virulent Semliki Forest virus overcomes antiviral type I interferon in resistant mouse CT-2A glioma. J. Virol. 89, 10637\u201310647 (2015).","journal-title":"J. Virol."},{"key":"2236_CR17","doi-asserted-by":"publisher","first-page":"944","DOI":"10.1016\/j.molonc.2013.05.004","volume":"7","author":"X Lun","year":"2013","unstructured":"Lun, X. et al. Double-deleted Vaccinia virus in virotherapy for refractory and metastatic pediatric solid tumors. Mol. Oncol. 7, 944\u2013954 (2013).","journal-title":"Mol. Oncol."},{"key":"2236_CR18","doi-asserted-by":"publisher","first-page":"2074","DOI":"10.1038\/mt.2013.195","volume":"21","author":"LM Whilding","year":"2013","unstructured":"Whilding, L. M. et al. Vaccinia virus induces programmed necrosis in ovarian cancer cells. Mol. Ther. 21, 2074\u20132086 (2013).","journal-title":"Mol. Ther."},{"key":"2236_CR19","doi-asserted-by":"publisher","first-page":"23","DOI":"10.1186\/s12865-016-0162-z","volume":"17","author":"V Hillerdal","year":"2016","unstructured":"Hillerdal, V., Boura, V. F., Bjorkelund, H., Andersson, K. & Essand, M. Avidity characterization of genetically engineered T-cells with novel and established approaches. BMC Immunol. 17, 23 (2016).","journal-title":"BMC Immunol."},{"key":"2236_CR20","doi-asserted-by":"publisher","first-page":"184","DOI":"10.1016\/j.virusres.2009.02.010","volume":"143","author":"A Sharma","year":"2009","unstructured":"Sharma, A., Li, X., Bangari, D. S. & Mittal, S. K. Adenovirus receptors and their implications in gene delivery. Virus Res. 143, 184\u2013194 (2009).","journal-title":"Virus Res."},{"key":"2236_CR21","doi-asserted-by":"publisher","first-page":"9","DOI":"10.1016\/j.virol.2011.04.017","volume":"416","author":"H Rodriguez-Rocha","year":"2011","unstructured":"Rodriguez-Rocha, H. et al. Adenoviruses induce autophagy to promote virus replication and oncolysis. Virology 416, 9\u201315 (2011).","journal-title":"Virology"},{"key":"2236_CR22","doi-asserted-by":"publisher","first-page":"625","DOI":"10.1093\/jnci\/djj161","volume":"98","author":"H Ito","year":"2006","unstructured":"Ito, H. et al. Autophagic cell death of malignant glioma cells induced by a conditionally replicating adenovirus. J. Natl Cancer Inst. 98, 625\u2013636 (2006).","journal-title":"J. Natl Cancer Inst."},{"key":"2236_CR23","doi-asserted-by":"publisher","first-page":"368","DOI":"10.1111\/cmi.12043","volume":"15","author":"C Richetta","year":"2013","unstructured":"Richetta, C. & Faure, M. Autophagy in antiviral innate immunity. Cell Microbiol. 15, 368\u2013376 (2013).","journal-title":"Cell Microbiol."},{"key":"2236_CR24","doi-asserted-by":"publisher","first-page":"1398","DOI":"10.1126\/science.1136880","volume":"315","author":"HK Lee","year":"2007","unstructured":"Lee, H. K., Lund, J. M., Ramanathan, B., Mizushima, N. & Iwasaki, A. Autophagy-dependent viral recognition by plasmacytoid dendritic cells. Science 315, 1398\u20131401 (2007).","journal-title":"Science"},{"key":"2236_CR25","doi-asserted-by":"publisher","first-page":"760","DOI":"10.1038\/nri3921","volume":"15","author":"GN Barber","year":"2015","unstructured":"Barber, G. N. STING: infection, inflammation and cancer. Nat. Rev. Immunol. 15, 760\u2013770 (2015).","journal-title":"Nat. Rev. Immunol."},{"key":"2236_CR26","doi-asserted-by":"publisher","first-page":"321","DOI":"10.4161\/auto.7.3.14583","volume":"7","author":"G Petrovski","year":"2011","unstructured":"Petrovski, G. et al. Phagocytosis of cells dying through autophagy induces inflammasome activation and IL-1beta release in human macrophages. Autophagy 7, 321\u2013330 (2011).","journal-title":"Autophagy"},{"key":"2236_CR27","doi-asserted-by":"publisher","first-page":"363","DOI":"10.1093\/intimm\/dxs161","volume":"25","author":"Q Wang","year":"2013","unstructured":"Wang, Q. et al. Pyroptotic cells externalize eat-me and release find-me signals and are efficiently engulfed by macrophages. Int. Immunol. 25, 363\u2013372 (2013).","journal-title":"Int. Immunol."},{"key":"2236_CR28","doi-asserted-by":"publisher","first-page":"1396","DOI":"10.1038\/cdd.2008.61","volume":"15","author":"C Urban","year":"2008","unstructured":"Urban, C. et al. Apoptosis induced by Semliki Forest virus is RNA replication dependent and mediated via Bak. Cell Death Differ. 15, 1396\u20131407 (2008).","journal-title":"Cell Death Differ."},{"key":"2236_CR29","doi-asserted-by":"publisher","first-page":"715","DOI":"10.1016\/S1074-7613(01)00147-9","volume":"14","author":"W Chen","year":"2001","unstructured":"Chen, W., Frank, M. E., Jin, W. & Wahl, S. M. TGF-beta released by apoptotic T cells contributes to an immunosuppressive milieu. Immunity 14, 715\u2013725 (2001).","journal-title":"Immunity"},{"key":"2236_CR30","doi-asserted-by":"publisher","first-page":"1316","DOI":"10.1038\/cdd.2011.4","volume":"18","author":"DV Krysko","year":"2011","unstructured":"Krysko, D. V. et al. TLR-2 and TLR-9 are sensors of apoptosis in a mouse model of doxorubicin-induced acute inflammation. Cell Death Differ. 18, 1316\u20131325 (2011).","journal-title":"Cell Death Differ."},{"key":"2236_CR31","doi-asserted-by":"publisher","first-page":"7941","DOI":"10.1158\/0008-5472.CAN-07-1622","volume":"67","author":"M Obeid","year":"2007","unstructured":"Obeid, M. et al. Leveraging the immune system during chemotherapy: moving calreticulin to the cell surface converts apoptotic death from \u201csilent\u201d to immunogenic. Cancer Res. 67, 7941\u20137944 (2007).","journal-title":"Cancer Res."},{"key":"2236_CR32","doi-asserted-by":"publisher","first-page":"1848","DOI":"10.1038\/sj.cdd.4402201","volume":"14","author":"M Obeid","year":"2007","unstructured":"Obeid, M. et al. Calreticulin exposure is required for the immunogenicity of gamma-irradiation and UVC light-induced apoptosis. Cell Death Differ. 14, 1848\u20131850 (2007).","journal-title":"Cell Death Differ."},{"key":"2236_CR33","doi-asserted-by":"publisher","first-page":"1112","DOI":"10.1016\/j.cell.2009.05.037","volume":"137","author":"YS Cho","year":"2009","unstructured":"Cho, Y. S. et al. Phosphorylation-driven assembly of the RIP1-RIP3 complex regulates programmed necrosis and virus-induced inflammation. Cell 137, 1112\u20131123 (2009).","journal-title":"Cell"},{"key":"2236_CR34","doi-asserted-by":"publisher","first-page":"798","DOI":"10.1016\/j.cell.2010.02.015","volume":"140","author":"L Zitvogel","year":"2010","unstructured":"Zitvogel, L., Kepp, O. & Kroemer, G. Decoding cell death signals in inflammation and immunity. Cell 140, 798\u2013804 (2010).","journal-title":"Cell"},{"key":"2236_CR35","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1003703","volume":"9","author":"K Tapia","year":"2013","unstructured":"Tapia, K. et al. Defective viral genomes arising in vivo provide critical danger signals for the triggering of lung antiviral immunity. PLoS Pathog. 9, e1003703 (2013).","journal-title":"PLoS Pathog."},{"key":"2236_CR36","doi-asserted-by":"publisher","first-page":"597","DOI":"10.3389\/fimmu.2016.00597","volume":"7","author":"QB Zha","year":"2016","unstructured":"Zha, Q. B. et al. ATP-induced inflammasome activation and pyroptosis is regulated by AMP-activated protein kinase in macrophages. Front Immunol. 7, 597 (2016).","journal-title":"Front Immunol."},{"key":"2236_CR37","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0040069","volume":"7","author":"G Ayna","year":"2012","unstructured":"Ayna, G. et al. ATP release from dying autophagic cells and their phagocytosis are crucial for inflammasome activation in macrophages. PLoS ONE 7, e40069 (2012).","journal-title":"PLoS ONE"},{"key":"2236_CR38","doi-asserted-by":"publisher","first-page":"1573","DOI":"10.1126\/science.1208347","volume":"334","author":"M Michaud","year":"2011","unstructured":"Michaud, M. et al. Autophagy-dependent anticancer immune responses induced by chemotherapeutic agents in mice. Science 334, 1573\u20131577 (2011).","journal-title":"Science"},{"key":"2236_CR39","doi-asserted-by":"publisher","first-page":"54","DOI":"10.1038\/nm1523","volume":"13","author":"M Obeid","year":"2007","unstructured":"Obeid, M. et al. Calreticulin exposure dictates the immunogenicity of cancer cell death. Nat. Med 13, 54\u201361 (2007).","journal-title":"Nat. Med"},{"key":"2236_CR40","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1084\/jem.192.4.565","volume":"192","author":"U Andersson","year":"2000","unstructured":"Andersson, U. et al. High mobility group 1 protein (HMG-1) stimulates proinflammatory cytokine synthesis in human monocytes. J. Exp. Med. 192, 565\u2013570 (2000).","journal-title":"J. Exp. Med."},{"key":"2236_CR41","doi-asserted-by":"publisher","first-page":"1050","DOI":"10.1038\/nm1622","volume":"13","author":"L Apetoh","year":"2007","unstructured":"Apetoh, L. et al. Toll-like receptor 4-dependent contribution of the immune system to anticancer chemotherapy and radiotherapy. Nat. Med. 13, 1050\u20131059 (2007).","journal-title":"Nat. Med."},{"key":"2236_CR42","doi-asserted-by":"publisher","first-page":"832","DOI":"10.1038\/ni.2376","volume":"13","author":"S Chiba","year":"2012","unstructured":"Chiba, S. et al. Tumor-infiltrating DCs suppress nucleic acid-mediated innate immune responses through interactions between the receptor TIM-3 and the alarmin HMGB1. Nat. Immunol. 13, 832\u2013842 (2012).","journal-title":"Nat. Immunol."},{"key":"2236_CR43","doi-asserted-by":"crossref","unstructured":"Di Pilato, M., Mejias-Perez, E., Sorzano, C. O. S., Esteban, M. Distinct roles of Vaccinia virus NF-kappaB inhibitor proteins A52, B15, and K7 in the immune response. J. Virol. 91, e00575 (2017).","DOI":"10.1128\/JVI.00575-17"},{"key":"2236_CR44","doi-asserted-by":"publisher","first-page":"153","DOI":"10.1016\/0092-8674(92)90274-G","volume":"71","author":"A Alcami","year":"1992","unstructured":"Alcami, A. & Smith, G. L. A soluble receptor for interleukin-1 beta encoded by Vaccinia virus: a novel mechanism of virus modulation of the host response to infection. Cell 71, 153\u2013167 (1992).","journal-title":"Cell"},{"key":"2236_CR45","doi-asserted-by":"publisher","first-page":"11230","DOI":"10.1128\/JVI.74.23.11230-11239.2000","volume":"74","author":"A Alcami","year":"2000","unstructured":"Alcami, A., Symons, J. A. & Smith, G. L. The Vaccinia virus soluble alpha\/beta interferon (IFN) receptor binds to the cell surface and protects cells from the antiviral effects of IFN. J. Virol. 74, 11230\u201311239 (2000).","journal-title":"J. Virol."},{"key":"2236_CR46","doi-asserted-by":"crossref","first-page":"6762","DOI":"10.4049\/jimmunol.163.12.6762","volume":"163","author":"J Engelmayer","year":"1999","unstructured":"Engelmayer, J. et al. Vaccinia virus inhibits the maturation of human dendritic cells: a novel mechanism of immune evasion. J. Immunol. 163, 6762\u20136768 (1999).","journal-title":"J. Immunol."},{"key":"2236_CR47","doi-asserted-by":"publisher","first-page":"9977","DOI":"10.1128\/JVI.00354-06","volume":"80","author":"L Deng","year":"2006","unstructured":"Deng, L., Dai, P., Ding, W., Granstein, R. D. & Shuman, S. Vaccinia virus infection attenuates innate immune responses and antigen presentation by epidermal dendritic cells. J. Virol. 80, 9977\u20139987 (2006).","journal-title":"J. Virol."},{"issue":"11","key":"2236_CR48","doi-asserted-by":"publisher","first-page":"eaal1713","DOI":"10.1126\/sciimmunol.aal1713","volume":"2","author":"Peihong Dai","year":"2017","unstructured":"Dai, P. et al. Intratumoral delivery of inactivated modified Vaccinia virus Ankara (iMVA) induces systemic antitumor immunity via STING and Batf3-dependent dendritic cells. Sci. Immunol. 2, eaa1713 (2017).","journal-title":"Science Immunology"},{"issue":"422","key":"2236_CR49","doi-asserted-by":"publisher","first-page":"eaao1641","DOI":"10.1126\/scitranslmed.aao1641","volume":"10","author":"Marie-Claude Bourgeois-Daigneault","year":"2018","unstructured":"Bourgeois-Daigneault, M. C. et al. Neoadjuvant oncolytic virotherapy before surgery sensitizes triple-negative breast cancer to immune checkpoint therapy. Sci. Transl. Med. 10, eaao1641 (2018).","journal-title":"Science Translational Medicine"},{"key":"2236_CR50","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2018.1442169","volume":"7","author":"S Gujar","year":"2018","unstructured":"Gujar, S., Pol, J. G. & Kroemer, G. Heating it up: Oncolytic viruses make tumors \u2018hot\u2019 and suitable for checkpoint blockade immunotherapies. Oncoimmunology 7, e1442169 (2018).","journal-title":"Oncoimmunology"},{"key":"2236_CR51","unstructured":"Samson, A. et al. Intravenous delivery of oncolytic reovirus to brain tumor patients immunologically primes for subsequent checkpoint blockade. Sci. Transl. Med. 10, eaam7577 (2018)."},{"key":"2236_CR52","doi-asserted-by":"publisher","first-page":"95","DOI":"10.1038\/nmeth734","volume":"2","author":"A Domi","year":"2005","unstructured":"Domi, A. & Moss, B. Engineering of a Vaccinia virus bacterial artificial chromosome in Escherichia coli by bacteriophage \u03bb-based recombination. Nat. Methods 2, 95 (2005).","journal-title":"Nat. Methods"},{"key":"2236_CR53","doi-asserted-by":"publisher","first-page":"455","DOI":"10.4049\/jimmunol.1401110","volume":"194","author":"DP Sester","year":"2015","unstructured":"Sester, D. P. et al. A novel flow cytometric method to assess inflammasome formation. J. Immunol. 194, 455\u2013462 (2015).","journal-title":"J. Immunol."},{"key":"2236_CR54","doi-asserted-by":"publisher","DOI":"10.1080\/2162402X.2017.1395126","volume":"7","author":"G Fotaki","year":"2018","unstructured":"Fotaki, G. et al. Pro-inflammatory allogeneic DCs promote activation of bystander immune cells and thereby license antigen-specific T-cell responses. Oncoimmunology 7, e1395126 (2018).","journal-title":"Oncoimmunology"}],"container-title":["Cell Death &amp; Disease"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.nature.com\/articles\/s41419-020-2236-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41419-020-2236-3","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.nature.com\/articles\/s41419-020-2236-3.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T13:38:45Z","timestamp":1695649125000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41419-020-2236-3"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,1,22]]},"references-count":54,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2020,1]]}},"alternative-id":["2236"],"URL":"https:\/\/doi.org\/10.1038\/s41419-020-2236-3","relation":{},"ISSN":["2041-4889"],"issn-type":[{"value":"2041-4889","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,1,22]]},"assertion":[{"value":"13 May 2019","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"7 November 2019","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"11 November 2019","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 January 2020","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"The authors declare that they have no conflict of interest.","order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}},{"value":"Buffy coats from healthy blood donors were collected at the blood center, Uppsala University hospital, Uppsala, Sweden. The samples were completely anonymized therefore no ethical permit is required. The Swedish Work Environment Authority has approved the work with viruses and all experiments were conducted under Biosafety level 2.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Ethics statement"}}],"article-number":"48"}}