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The nexin-dynein regulatory complex (N-DRC) is a crucial structural component of motile cilia and flagella, present across various species from\n                    <jats:italic>Chlamydomonas<\/jats:italic>\n                    to humans. Defects in N-DRC components lead to multiple PCD symptoms, including sinusitis and male infertility. However, the phenotypic expression of N-DRC defects varies significantly among individuals, and there has been a lack of systematic study of core N-DRC components in mammals. Utilizing\n                    <jats:italic>Drc1-4<\/jats:italic>\n                    and\n                    <jats:italic>Drc7<\/jats:italic>\n                    knockout mice, this study systematically reveals the roles and assembly process of core N-DRC components in ependymal cilia, respiratory cilia, and sperm flagella. The findings show that core N-DRC components are crucial for the survival of mice on a purebred genetic background. In mixed genetic background mice, N-DRC defects impair the motility of motile cilia and the stability of flagellar axonemes. Additionally, a novel role of the N-DRC specific component (A-kinase anchoring protein 3) AKAP3 in regulating sperm phosphorylation was discovered. Collectively, our results provide a comprehensive understanding of the core N-DRC components in mammalian cilia and flagella.\n                  <\/jats:p>","DOI":"10.1038\/s41419-025-07506-2","type":"journal-article","created":{"date-parts":[[2025,3,15]],"date-time":"2025-03-15T09:39:46Z","timestamp":1742031586000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Core N-DRC components play a crucial role in embryonic development and postnatal organ development"],"prefix":"10.1038","volume":"16","author":[{"given":"Chuan","family":"Ren","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuya","family":"Sun","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiajie","family":"Zhu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shushu","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuhui","family":"Bian","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Wang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3488-636X","authenticated-orcid":false,"given":"Jintao","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6499-7899","authenticated-orcid":false,"given":"Mingxi","family":"Liu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,3,15]]},"reference":[{"key":"7506_CR1","doi-asserted-by":"publisher","first-page":"107s","DOI":"10.1083\/jcb.91.3.107s","volume":"91","author":"IR Gibbons","year":"1981","unstructured":"Gibbons IR. 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