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We here investigated the distribution of 2,3-disubstituted butenolides (SRB-type butenolides) among randomly selected 122 <jats:italic>Streptomyces<\/jats:italic> strains using two approaches; (1) feeding of their culture extracts into an <jats:italic>srrX<\/jats:italic>-deficient strain KA20 of <jats:italic>S. rochei<\/jats:italic>, and (2) co-fermentation with strain KA20. All the randomly selected donor strains, except for <jats:italic>Streptomyces cellostaticus<\/jats:italic> (a LC and LM producer), failed to restore LC and LM production in strain KA20. These findings strongly revealed the rare distribution of SRB-type butenolide molecules in <jats:italic>Streptomyces<\/jats:italic> species. One of the SRB-type butenolide, SAB1, an inducing molecule for nikkomycin production in <jats:italic>Streptomyces ansochromogenes<\/jats:italic>, was unable to restore antibiotic production in strain KA20 even at 1\u2009mM concentration. Furthermore, we noticed the accumulation of 4-dehydroxy-SRB1 as a novel compound when SRB1 was fed into strain KA20. Purified 4-dehydroxy-SRB1 has no inducing activity of antibiotic production in strain KA20 even at 1000-fold higher concentration (50\u2009\u00b5M) against a minimum inducing concentration of natural SRB1 (40\u2009nM). These findings suggested the importance of the length of a hydrocarbon chain attached at C-2 and a hydroxyl group at C-4 for inducing activity in <jats:italic>S. rochei<\/jats:italic>.<\/jats:p>","DOI":"10.1038\/s41429-025-00840-9","type":"journal-article","created":{"date-parts":[[2025,6,12]],"date-time":"2025-06-12T02:20:33Z","timestamp":1749694833000},"page":"488-499","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Rare distribution of butenolide-type signaling molecules among Streptomyces strains and functional importance as inducing factors for secondary metabolite production in Streptomyces rochei 7434AN4"],"prefix":"10.1038","volume":"78","author":[{"given":"Asahi","family":"Hirata","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miho","family":"Sumiyoshi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hazuki","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Momoko","family":"Akimoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Hannah Rose A.","family":"Padayao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuto","family":"Eguchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maki","family":"Matsuura","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1907-8823","authenticated-orcid":false,"given":"Miyuki","family":"Otsuka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3493-5838","authenticated-orcid":false,"given":"Kuninobu","family":"Inada","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aiko","family":"Teshima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5129-0042","authenticated-orcid":false,"given":"Kenji","family":"Arakawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2025,6,12]]},"reference":[{"key":"840_CR1","doi-asserted-by":"publisher","first-page":"208","DOI":"10.1016\/j.mib.2005.02.016","volume":"8","author":"MJ Bibb","year":"2005","unstructured":"Bibb MJ. 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