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To date, pathogenic variants in <jats:italic>AGBL5<\/jats:italic> have been associated only with isolated retinitis pigmentosa (RP). Hearing loss has not been reported in <jats:italic>AGBL5<\/jats:italic>-caused retinal disease. In this study, we performed exome sequencing in probands of eight unrelated families from Italy, Spain, Palestine, Switzerland, and Greece. All subjects had a clinical diagnosis of (suspected) Usher syndrome type II for the concurrent presence of RP and post-verbal sensorineural hearing loss (SNHL) that ranged from mild to moderate.We identified biallelic sequence variants in <jats:italic>AGBL5<\/jats:italic> in all analysed subjects. Four of the identified variants were novel. The variants co-segregated with the retinal and auditory phenotypes in additional affected family members. We did not detect any causative variants in known deafness or Usher syndrome genes that could explain the patients\u2019 hearing loss. We therefore conclude that SNHL is a feature of a syndromic presentation of <jats:italic>AGBL5<\/jats:italic> retinopathy. This study provides the first evidence that mutations in <jats:italic>AGBL5<\/jats:italic> can cause syndromic RP forms associated with hearing loss, probably due to dysfunction of sensory cilia in the retina and the inner ear.<\/jats:p>","DOI":"10.1038\/s41431-024-01768-8","type":"journal-article","created":{"date-parts":[[2024,12,13]],"date-time":"2024-12-13T18:34:06Z","timestamp":1734114846000},"page":"727-737","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Variants in the\u00a0AGBL5 gene are responsible for autosomal recessive Retinitis pigmentosa with hearing 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