{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T03:02:09Z","timestamp":1778641329881,"version":"3.51.4"},"reference-count":16,"publisher":"Springer Science and Business Media LLC","issue":"8","license":[{"start":{"date-parts":[[2018,5,10]],"date-time":"2018-05-10T00:00:00Z","timestamp":1525910400000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Eye"],"published-print":{"date-parts":[[2018,8]]},"DOI":"10.1038\/s41433-018-0089-0","type":"journal-article","created":{"date-parts":[[2018,5,8]],"date-time":"2018-05-08T13:14:46Z","timestamp":1525785286000},"page":"1380-1386","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Three-dimensional tubule formation assay as therapeutic screening model for ocular microvascular disorders"],"prefix":"10.1038","volume":"32","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6541-312X","authenticated-orcid":false,"given":"Mahnaz","family":"Shariatzadeh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maarten M.","family":"Brandt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Josianne C.","family":"ten Berge","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aniki","family":"Rothova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pieter J. M.","family":"Leenen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Willem A.","family":"Dik","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,5,10]]},"reference":[{"key":"89_CR1","doi-asserted-by":"publisher","first-page":"67","DOI":"10.1016\/j.preteyeres.2015.06.002","volume":"49","author":"PA Campochiaro","year":"2015","unstructured":"Campochiaro PA. Molecular pathogenesis of retinal and choroidal vascular diseases. Prog Retin Eye Res. 2015;49:67\u201381.","journal-title":"Prog Retin Eye Res"},{"key":"89_CR2","doi-asserted-by":"publisher","first-page":"30","DOI":"10.1016\/j.exer.2014.10.021","volume":"133","author":"PN Bishop","year":"2015","unstructured":"Bishop PN. The role of extracellular matrix in retinal vascular development and preretinal neovascularization. Exp Eye Res. 2015;133:30\u20136.","journal-title":"Exp Eye Res"},{"key":"89_CR3","doi-asserted-by":"publisher","first-page":"441","DOI":"10.1124\/pr.114.010215","volume":"67","author":"JR Beijnum van","year":"2015","unstructured":"van Beijnum JR, Nowak-Sliwinska P, Huijbers EJ, Thijssen VL, Griffioen AW. The great escape; the hallmarks of resistance to antiangiogenic therapy. Pharmacol Rev. 2015;67:441\u201361.","journal-title":"Pharmacol Rev"},{"key":"89_CR4","doi-asserted-by":"publisher","first-page":"4","DOI":"10.1159\/000320006","volume":"46","author":"MJ Siemerink","year":"2010","unstructured":"Siemerink MJ, Augustin AJ, Schlingemann RO. Mechanisms of ocular angiogenesis and its molecular mediators. Dev Ophthalmol. 2010;46:4\u201320.","journal-title":"Dev Ophthalmol"},{"key":"89_CR5","doi-asserted-by":"publisher","first-page":"1","DOI":"10.1038\/s41598-016-0028-x","volume":"7","author":"T Yan","year":"2017","unstructured":"Yan T, Wang M, Xu Z, Huang C, Zhou X, Jiang E, et al. Up-regulation of syncytin-1 contributes to TNF-\u03b1-enhanced fusion between OSCC and HUVECs partly via Wnt\/\u03b2-catenin-dependent pathway. Sci Rep. 2017;7:1\u201312.","journal-title":"Sci Rep"},{"key":"89_CR6","doi-asserted-by":"publisher","first-page":"H299","DOI":"10.1152\/ajpheart.00578.2015","volume":"311","author":"ZC Zhou","year":"2016","unstructured":"Zhou ZC, Chrifi I, Xu YJ, Pernow J, Duncker DJ, Merkus D, et al. Uridine adenosine tetraphosphate acts as a proangiogenic factor in vitro through purinergic P2Y receptors. Am J Physiol-Heart C. 2016;311:H299\u2013309.","journal-title":"Am J Physiol-Heart C"},{"key":"89_CR7","doi-asserted-by":"publisher","first-page":"4720","DOI":"10.1182\/blood-2010-05-286872","volume":"116","author":"AN Stratman","year":"2010","unstructured":"Stratman AN, Schwindt AE, Malotte KM, Davis GE. Endothelial-derived PDGF-BB and HB-EGF coordinately regulate pericyte recruitment during vasculogenic tube assembly and stabilization. Blood. 2010;116:4720\u201330.","journal-title":"Blood"},{"key":"89_CR8","doi-asserted-by":"publisher","first-page":"5356","DOI":"10.1038\/onc.2017.145","volume":"36","author":"C Zhu","year":"2017","unstructured":"Zhu C, Chrifi I, Mustafa D, van der Weiden M, Leenen PJM, Duncker DJ, et al. CECR1-mediated cross talk between macrophages and vascular mural cells promotes neovascularization in malignant glioma. Oncogene. 2017;36:5356\u201368.","journal-title":"Oncogene"},{"key":"89_CR9","doi-asserted-by":"publisher","first-page":"1098","DOI":"10.1161\/ATVBAHA.116.308792","volume":"37","author":"I Chrifi","year":"2017","unstructured":"Chrifi I, Louzao-Martinez L, Brandt M, van Dijk CGM, Burgisser P, Zhu C, et al. CMTM3 (CKLF-like marvel transmembrane domain 3) mediates angiogenesis by regulating cell surface availability of ve-cadherin in endothelial adherens junctions. Arterioscler Thromb Vasc Biol. 2017;37:1098\u2013114.","journal-title":"Arterioscler Thromb Vasc Biol"},{"key":"89_CR10","doi-asserted-by":"publisher","first-page":"654","DOI":"10.1007\/s00268-006-0763-4","volume":"31","author":"AA Ucuzian","year":"2007","unstructured":"Ucuzian AA, Greisler HP. In vitro models of angiogenesis. World J Surg. 2007;31:654\u201363.","journal-title":"World J Surg"},{"key":"89_CR11","doi-asserted-by":"publisher","first-page":"429","DOI":"10.1007\/s10456-013-9398-x","volume":"17","author":"S Rezzola","year":"2014","unstructured":"Rezzola S, Belleri M, Gariano G, Ribatti D, Costagliola C, Semeraro F, et al. In vitro and ex vivo retina angiogenesis assays. Angiogenesis. 2014;17:429\u201342.","journal-title":"Angiogenesis"},{"key":"89_CR12","doi-asserted-by":"publisher","first-page":"761","DOI":"10.1016\/j.exer.2011.09.010","volume":"93","author":"EA Stewart","year":"2011","unstructured":"Stewart EA, Samaranayake GJ, Browning AC, Hopkinson A, Amoaku WM. Comparison of choroidal and retinal endothelial cells: characteristics and response to VEGF isoforms and anti-VEGF treatments. Exp Eye Res. 2011;93:761\u20136.","journal-title":"Exp Eye Res"},{"key":"89_CR13","doi-asserted-by":"publisher","first-page":"3488","DOI":"10.1091\/mbc.e04-11-1029","volume":"16","author":"E Im","year":"2005","unstructured":"Im E, Venkatakrishnan A, Kazlauskas A. Cathepsin B regulates the intrinsic angiogenic threshold of endothelial cells. Mol Biol Cell. 2005;16:3488\u2013500.","journal-title":"Mol Biol Cell"},{"key":"89_CR14","first-page":"2378","volume":"41","author":"A Shafiee","year":"2000","unstructured":"Shafiee A, Penn JS, Krutzsch HC, Inman JK, Roberts DD, Blake DA. Inhibition of retinal angiogenesis by peptides derived from thrombospondin-1. Invest Ophthalmol Vis Sci. 2000;41:2378\u201388.","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"89_CR15","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1016\/j.exer.2013.04.014","volume":"112","author":"S Rezzola","year":"2013","unstructured":"Rezzola S, Belleri M, Ribatti D, Costagliola C, Presta M, Semeraro F. A novel ex vivo murine retina angiogenesis (EMRA) assay. Exp Eye Res. 2013;112:51\u20136.","journal-title":"Exp Eye Res"},{"key":"89_CR16","doi-asserted-by":"publisher","first-page":"102","DOI":"10.1016\/j.preteyeres.2012.08.004","volume":"32","author":"AS Bharadwaj","year":"2013","unstructured":"Bharadwaj AS, Appukuttan B, Wilmarth PA, Pan Y, Stempel AJ, Chipps TJ, et al. Role of the retinal vascular endothelial cell in ocular disease. Prog Retin Eye Res. 2013;32:102\u201380.","journal-title":"Prog Retin Eye Res"}],"container-title":["Eye"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/s41433-018-0089-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/s41433-018-0089-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/s41433-018-0089-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,20]],"date-time":"2023-05-20T21:04:16Z","timestamp":1684616656000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41433-018-0089-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,5,10]]},"references-count":16,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2018,8]]}},"alternative-id":["89"],"URL":"https:\/\/doi.org\/10.1038\/s41433-018-0089-0","relation":{},"ISSN":["0950-222X","1476-5454"],"issn-type":[{"value":"0950-222X","type":"print"},{"value":"1476-5454","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,5,10]]},"assertion":[{"value":"9 October 2017","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"22 January 2018","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"20 February 2018","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"10 May 2018","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}