{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,1]],"date-time":"2026-06-01T20:00:04Z","timestamp":1780344004752,"version":"3.54.1"},"reference-count":29,"publisher":"Springer Science and Business Media LLC","issue":"10","license":[{"start":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T00:00:00Z","timestamp":1588896000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"},{"start":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T00:00:00Z","timestamp":1588896000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/www.springer.com\/tdm"}],"content-domain":{"domain":["link.springer.com"],"crossmark-restriction":false},"short-container-title":["Hypertens Res"],"published-print":{"date-parts":[[2020,10]]},"DOI":"10.1038\/s41440-020-0450-0","type":"journal-article","created":{"date-parts":[[2020,5,8]],"date-time":"2020-05-08T02:03:26Z","timestamp":1588903406000},"page":"1079-1088","update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["V-ATPase blockade reduces renal gluconeogenesis and improves insulin secretion in type 2 diabetic rats"],"prefix":"10.1038","volume":"43","author":[{"given":"Jun","family":"Hirao","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Akihiro","family":"Tojo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saaya","family":"Hatakeyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Satonaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toshihiko","family":"Ishimitsu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2020,5,8]]},"reference":[{"key":"450_CR1","first-page":"2704","volume":"44","author":"KM Madsen","year":"1985","unstructured":"Madsen KM, Tisher CC. Structure-function relationships in H+-secreting epithelia. Federation Proc. 1985;44:2704\u20139.","journal-title":"Federation Proc"},{"key":"450_CR2","doi-asserted-by":"crossref","first-page":"2661","DOI":"10.1002\/elps.1150181423","volume":"18","author":"V Marshansky","year":"1997","unstructured":"Marshansky V, Bourgoin S, Londono I, Bendayan M, Maranda B, Vinay P. Receptor-mediated endocytosis in kidney proximal tubules: recent advances and hypothesis. Electrophoresis. 1997;18:2661\u201376.","journal-title":"Electrophoresis"},{"key":"450_CR3","doi-asserted-by":"crossref","first-page":"1263","DOI":"10.1152\/physrev.00045.2003","volume":"84","author":"CA Wagner","year":"2004","unstructured":"Wagner CA, Finberg KE, Breton S, Marshansky V, Brown D, Geibel JP. Renal vacuolar H+-ATPase. Physiol Rev. 2004;84:1263\u2013314.","journal-title":"Physiol Rev"},{"key":"450_CR4","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1247\/csf.28.455","volume":"28","author":"GH Sun-Wada","year":"2003","unstructured":"Sun-Wada GH, Wada Y, Futai M. Lysosome and lysosome-related organelles responsible for specialized functions in higher organisms, with special emphasis on vacuolar-type proton ATPase. Cell Struct Funct. 2003;28:455\u201363.","journal-title":"Cell Struct Funct"},{"key":"450_CR5","doi-asserted-by":"crossref","first-page":"1071","DOI":"10.1152\/physrev.00035.2015","volume":"96","author":"L Stransky","year":"2016","unstructured":"Stransky L, Cotter K, Forgac M. The function of V-ATPases in cancer. Physiol Rev. 2016;96:1071\u201391.","journal-title":"Physiol Rev"},{"key":"450_CR6","doi-asserted-by":"crossref","first-page":"89","DOI":"10.1007\/s00795-017-0175-6","volume":"51","author":"A Tojo","year":"2018","unstructured":"Tojo A, Hatakeyama S, Nangaku M, Ishimitsu T. H(+)-ATPase blockade reduced renal gluconeogenesis and plasma glucose in a diabetic rat model. Med Mol Morphol. 2018;51:89\u201395.","journal-title":"Med Mol Morphol"},{"key":"450_CR7","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1038\/ki.1975.55","volume":"7","author":"FI Bennett","year":"1975","unstructured":"Bennett FI, Alexander JE, Roobol A, Alleyne GA. Effect of starvation on renal metabolism in the rat. Kidney Int. 1975;7:380\u20134.","journal-title":"Kidney Int"},{"key":"450_CR8","doi-asserted-by":"crossref","first-page":"574","DOI":"10.1172\/JCI106016","volume":"48","author":"OE Owen","year":"1969","unstructured":"Owen OE, Felig P, Morgan AP, Wahren J, Cahill GF Jr. Liver and kidney metabolism during prolonged starvation. J Clin Invest. 1969;48:574\u201383.","journal-title":"J Clin Invest"},{"key":"450_CR9","first-page":"F246","volume":"235","author":"HB Burch","year":"1978","unstructured":"Burch HB, Narins RG, Chu C, Fagioli S, Choi S, McCarthy W, et al. Distribution along the rat nephron of three enzymes of gluconeogenesis in acidosis and starvation. Am J Physiol. 1978;235:F246\u2013F253.","journal-title":"Am J Physiol"},{"key":"450_CR10","doi-asserted-by":"crossref","first-page":"F1","DOI":"10.1152\/ajprenal.00067.2014","volume":"307","author":"NP Curthoys","year":"2014","unstructured":"Curthoys NP, Gstraunthaler G. pH-responsive, gluconeogenic renal epithelial LLC-PK1-FBPase+cells: a versatile in vitro model to study renal proximal tubule metabolism and function. Am J Physiol Ren Physiol. 2014;307:F1\u2013F11.","journal-title":"Am J Physiol Ren Physiol"},{"key":"450_CR11","doi-asserted-by":"crossref","first-page":"E428","DOI":"10.1152\/ajpendo.00116.2001","volume":"282","author":"C Meyer","year":"2002","unstructured":"Meyer C, Stumvoll M, Dostou J, Welle S, Haymond M, Gerich J. Renal substrate exchange and gluconeogenesis in normal postabsorptive humans. Am J Physiol Endocrinol Metab. 2002;282:E428\u2013E434.","journal-title":"Am J Physiol Endocrinol Metab"},{"key":"450_CR12","doi-asserted-by":"crossref","first-page":"364","DOI":"10.1172\/JCI105993","volume":"48","author":"GA Alleyne","year":"1969","unstructured":"Alleyne GA, Scullard GH. Renal metabolic response to acid base changes. I. Enzymatic control of ammoniagenesis in the rat. J Clin Invest. 1969;48:364\u201370.","journal-title":"J Clin Invest"},{"key":"450_CR13","doi-asserted-by":"crossref","first-page":"31308","DOI":"10.1074\/jbc.272.50.31308","volume":"272","author":"CA Aspinwall","year":"1997","unstructured":"Aspinwall CA, Brooks SA, Kennedy RT, Lakey JR. Effects of intravesicular H+ and extracellular H+ and Zn2+ on insulin secretion in pancreatic beta cells. J Biol Chem. 1997;272:31308\u2013314.","journal-title":"J Biol Chem"},{"key":"450_CR14","doi-asserted-by":"crossref","first-page":"4531","DOI":"10.1242\/jcs.03234","volume":"119","author":"GH Sun-Wada","year":"2006","unstructured":"Sun-Wada GH, Toyomura T, Murata Y, Yamamoto A, Futai M, Wada Y. The a3 isoform of V-ATPase regulates insulin secretion from pancreatic beta-cells. J Cell Sci. 2006;119:4531\u201340.","journal-title":"J Cell Sci"},{"key":"450_CR15","doi-asserted-by":"crossref","first-page":"25045","DOI":"10.1074\/jbc.M115.648592","volume":"290","author":"FF Dai","year":"2015","unstructured":"Dai FF, Bhattacharjee A, Liu Y, Batchuluun B, Zhang M, Wang XS, et al. A novel GLP1 receptor interacting protein ATP6ap2 regulates insulin secretion in pancreatic beta cells. J Biol Chem. 2015;290:25045\u201361.","journal-title":"J Biol Chem"},{"key":"450_CR16","doi-asserted-by":"crossref","first-page":"901","DOI":"10.2337\/diab.33.9.901","volume":"33","author":"J Levy","year":"1984","unstructured":"Levy J, Gavin JR 3rd, Fausto A, Gingerich R, LAvioli LV. Impaired insulin action in rats with non-insulin-dependent diabetes. Diabetes. 1984;33:901\u20136.","journal-title":"Diabetes"},{"key":"450_CR17","doi-asserted-by":"crossref","first-page":"374","DOI":"10.1210\/jcem-68-2-374","volume":"68","author":"E Bonora","year":"1989","unstructured":"Bonora E, Moghetti P, Zancanaro C, Cigolini M, Querena M, Cacciatori V, et al. Estimates of in vivo insulin action in man: comparison of insulin tolerance tests with euglycemic and hyperglycemic glucose clamp studies. J Clin Endocrinol Metab. 1989;68:374\u20138.","journal-title":"J Clin Endocrinol Metab"},{"key":"450_CR18","doi-asserted-by":"crossref","first-page":"103","DOI":"10.2147\/DMSO.S78771","volume":"8","author":"A Tojo","year":"2015","unstructured":"Tojo A, Hatakeyama S, Kinugasa S, Nangaku M. Angiotensin receptor blocker telmisartan suppresses renal gluconeogenesis during starvation. Diabetes, Metab Syndr Obes: Targets Ther. 2015;8:103\u201313.","journal-title":"Diabetes, Metab Syndr Obes: Targets Ther"},{"key":"450_CR19","doi-asserted-by":"crossref","first-page":"1574","DOI":"10.1038\/nprot.2010.123","volume":"5","author":"RD Unwin","year":"2010","unstructured":"Unwin RD, Griffiths JR, Whetton AD. Simultaneous analysis of relative protein expression levels across multiple samples using iTRAQ isobaric tags with 2D nano LC-MS\/MS. Nat Protoc. 2010;5:1574\u201382.","journal-title":"Nat Protoc"},{"key":"450_CR20","doi-asserted-by":"crossref","first-page":"86","DOI":"10.1007\/s00795-016-0151-6","volume":"50","author":"A Tojo","year":"2017","unstructured":"Tojo A, Hatakeyama S, Kinugasa S, Fukuda S, Sakai T. Enhanced podocyte vesicle transport in the nephrotic rat. Med Mol Morphol. 2017;50:86\u201393.","journal-title":"Med Mol Morphol"},{"key":"450_CR21","doi-asserted-by":"crossref","first-page":"73","DOI":"10.1016\/j.redox.2016.11.004","volume":"11","author":"M Redmann","year":"2017","unstructured":"Redmann M, Benavides GA, Berryhill TF, Wani WY, Ouyang X, Johnson MS, et al. Inhibition of autophagy with bafilomycin and chloroquine decreases mitochondrial quality and bioenergetic function in primary neurons. Redox Biol. 2017;11:73\u201381.","journal-title":"Redox Biol"},{"key":"450_CR22","doi-asserted-by":"crossref","first-page":"451","DOI":"10.1016\/j.bbrc.2009.03.051","volume":"382","author":"YC Wu","year":"2009","unstructured":"Wu YC, Wu WK, Li Y, Yu L, Li ZJ, Wong CC, et al. Inhibition of macroautophagy by bafilomycin A1 lowers proliferation and induces apoptosis in colon cancer cells. Biochem Biophys Res Commun. 2009;382:451\u20136.","journal-title":"Biochem Biophys Res Commun"},{"key":"450_CR23","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.15252\/embr.201643354","volume":"18","author":"V Giorgio","year":"2017","unstructured":"Giorgio V, Burchell V, Schiavone M, Bassot C, Minervini G, Petronilli V, et al. Ca(2+) binding to F-ATP synthase beta subunit triggers the mitochondrial permeability transition. EMBO Rep. 2017;18:1065\u201376.","journal-title":"EMBO Rep"},{"key":"450_CR24","doi-asserted-by":"crossref","first-page":"9112","DOI":"10.1073\/pnas.1505411112","volume":"112","author":"N Baker","year":"2015","unstructured":"Baker N, Hamilton G, Wilkes JM, Hutchinson S, Barrett MP, Horn D. Vacuolar ATPase depletion affects mitochondrial ATPase function, kinetoplast dependency, and drug sensitivity in trypanosomes. Proc Natl Acad Sci USA. 2015;112:9112\u20137.","journal-title":"Proc Natl Acad Sci USA"},{"key":"450_CR25","doi-asserted-by":"crossref","first-page":"136","DOI":"10.1111\/j.1464-5491.2009.02894.x","volume":"27","author":"JE Gerich","year":"2010","unstructured":"Gerich JE. Role of the kidney in normal glucose homeostasis and in the hyperglycaemia of diabetes mellitus: therapeutic implications. Diabet Med. 2010;27:136\u201342.","journal-title":"Diabet Med"},{"key":"450_CR26","doi-asserted-by":"crossref","first-page":"908","DOI":"10.1194\/jlr.M013375","volume":"52","author":"H Guo","year":"2011","unstructured":"Guo H, Li D, Ling W, Feng X, Xia M. Anthocyanin inhibits high glucose-induced hepatic mtGPAT1 activation and prevents fatty acid synthesis through PKCzeta. J Lipid Res. 2011;52:908\u201322.","journal-title":"J Lipid Res"},{"key":"450_CR27","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/S0079-6603(08)60896-X","volume":"60","author":"HS Sul","year":"1998","unstructured":"Sul HS, Smas CM, Wang D, Chen L. Regulation of fat synthesis and adipose differentiation. Prog Nucleic Acid Res Mol Biol. 1998;60:317\u201345.","journal-title":"Prog Nucleic Acid Res Mol Biol"},{"key":"450_CR28","doi-asserted-by":"crossref","first-page":"730","DOI":"10.1161\/CIRCRESAHA.117.312422","volume":"122","author":"L Ren","year":"2018","unstructured":"Ren L, Sun Y, Lu H, Ye D, Han L, Wang N. et al. (Pro) renin receptor inhibition reprograms hepatic lipid metabolism and protects mice from diet-induced obesity and hepatosteatosis. Circ Res. 2018;122:730\u201341.","journal-title":"Circ Res"},{"key":"450_CR29","doi-asserted-by":"crossref","first-page":"587","DOI":"10.1042\/BSR20120085","volume":"32","author":"AV Zhdanov","year":"2012","unstructured":"Zhdanov AV, Dmitriev RI, Papkovsky DB. Bafilomycin A1 activates HIF-dependent signalling in human colon cancer cells via mitochondrial uncoupling. Biosci Rep. 2012;32:587\u201395.","journal-title":"Biosci Rep"}],"container-title":["Hypertension Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/www.nature.com\/articles\/s41440-020-0450-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/s41440-020-0450-0","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/www.nature.com\/articles\/s41440-020-0450-0.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,5,20]],"date-time":"2023-05-20T21:24:28Z","timestamp":1684617868000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.nature.com\/articles\/s41440-020-0450-0"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,5,8]]},"references-count":29,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2020,10]]}},"alternative-id":["450"],"URL":"https:\/\/doi.org\/10.1038\/s41440-020-0450-0","relation":{},"ISSN":["0916-9636","1348-4214"],"issn-type":[{"value":"0916-9636","type":"print"},{"value":"1348-4214","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,5,8]]},"assertion":[{"value":"25 January 2020","order":1,"name":"received","label":"Received","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"28 March 2020","order":2,"name":"revised","label":"Revised","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"30 March 2020","order":3,"name":"accepted","label":"Accepted","group":{"name":"ArticleHistory","label":"Article History"}},{"value":"8 May 2020","order":4,"name":"first_online","label":"First Online","group":{"name":"ArticleHistory","label":"Article History"}},{"order":1,"name":"Ethics","group":{"name":"EthicsHeading","label":"Compliance with ethical standards"}},{"value":"The authors declare that they have no conflict of interest.","order":2,"name":"Ethics","group":{"name":"EthicsHeading","label":"Conflict of interest"}}]}}