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We demonstrate that the Wiskott\u2013Aldrich syndrome verprolin-homologous protein (WAVE) complex 2 is required for this transition, as complex degradation via deletion of its scaffold <jats:italic>Hem-1<\/jats:italic> causes the premature exhaustion of neonatal BM hematopoietic stem cells (HSCs). This exhaustion of BM HSC is due to the failure of BM engraftment of <jats:italic>Hem-1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> FL HSCs, causing early death. The <jats:italic>Hem-1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> FL HSC engraftment defect is not due to the lack of the canonical function of the WAVE2 complex, the regulation of actin polymerization, because FL HSCs from <jats:italic>Hem-1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> mice exhibit no defects in chemotaxis, BM homing, or adhesion. Rather, the failure of <jats:italic>Hem-1<\/jats:italic><jats:sup>\u2212\/\u2212<\/jats:sup> FL HSC engraftment in the marrow is due to the loss of c-Abl survival signaling from degradation of the WAVE2 complex. However, c-Abl activity is dispensable for the engraftment of adult BM HSCs into the BM. These findings reveal a novel function of the WAVE2 complex and define a mechanism for FL HSC fitness in the embryonic BM niche.<\/jats:p>","DOI":"10.1038\/s41467-018-04716-5","type":"journal-article","created":{"date-parts":[[2018,6,12]],"date-time":"2018-06-12T15:03:17Z","timestamp":1528815797000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["The Wave2 scaffold Hem-1 is required for transition of fetal liver hematopoiesis to bone marrow"],"prefix":"10.1038","volume":"9","author":[{"given":"Lijian","family":"Shao","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianhui","family":"Chang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Feng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyan","family":"Wang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elizabeth A.","family":"Williamson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amir","family":"Schajnovitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David","family":"Scadden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4331-4099","authenticated-orcid":false,"given":"Luke J.","family":"Mortensen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charles P.","family":"Lin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9963-430X","authenticated-orcid":false,"given":"Linheng","family":"Li","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ariel","family":"Paulson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Downing","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daohong","family":"Zhou","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"Hromas","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"297","published-online":{"date-parts":[[2018,6,18]]},"reference":[{"key":"4716_CR1","doi-asserted-by":"publisher","first-page":"411","DOI":"10.1016\/j.exphem.2013.01.009","volume":"41","author":"J Ciriza","year":"2013","unstructured":"Ciriza, J. et al. 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