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Within such environments, the bacterium must defend itself against predatory protozoan grazers. Amoebae are prominent grazers, with\n                    <jats:italic>Acanthamoeba castellanii<\/jats:italic>\n                    being one of the best-studied aquatic amoebae. We previously showed that\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    resists digestion by\n                    <jats:italic>A. castellanii<\/jats:italic>\n                    and establishes a replication niche within the host\u2019s osmoregulatory organelle. In this study, we decipher the molecular mechanisms involved in the maintenance of\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    \u2019s intra-amoebal replication niche and its ultimate escape from the succumbed host. We demonstrate that minor virulence features important for disease in mammals, such as extracellular enzymes and flagellum-based motility, have a key role in the replication and transmission of\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    in its aqueous environment. This work, therefore, describes new mechanisms that provide the pathogen with a fitness advantage in its primary habitat, which may have contributed to the emergence of these minor virulence factors in the species\n                    <jats:italic>V. cholerae<\/jats:italic>\n                    .\n                  <\/jats:p>","DOI":"10.1038\/s41467-018-05976-x","type":"journal-article","created":{"date-parts":[[2018,8,21]],"date-time":"2018-08-21T06:46:44Z","timestamp":1534834004000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Molecular insights into Vibrio cholerae\u2019s intra-amoebal host-pathogen interactions"],"prefix":"10.1038","volume":"9","author":[{"given":"Charles","family":"Van der Henst","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey Sophie","family":"Vanhove","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nat\u00e1lia Carolina","family":"Drebes D\u00f6rr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Stutzmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Candice","family":"Stoudmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phanie","family":"Clerc","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tiziana","family":"Scrignari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Maclachlan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2956-9052","authenticated-orcid":false,"given":"Graham","family":"Knott","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7024-1489","authenticated-orcid":false,"given":"Melanie","family":"Blokesch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,8,27]]},"reference":[{"key":"5976_CR1","doi-asserted-by":"crossref","first-page":"1301","DOI":"10.1128\/MMBR.62.4.1301-1314.1998","volume":"62","author":"SM Faruque","year":"1998","unstructured":"Faruque, S. 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