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The Xi shows weakened topologically associated domains and is instead organized into megadomains and superloops directed by the noncoding loci,\n                    <jats:italic>Dxz4<\/jats:italic>\n                    and\n                    <jats:italic>Firre<\/jats:italic>\n                    . Their functional significance is presently unclear, though one study suggests that they permit Xi genes to escape silencing. Here, we find that megadomains do not precede Xist expression or Xi gene silencing. Deleting\n                    <jats:italic>Dxz4<\/jats:italic>\n                    disrupts the sharp megadomain border, whereas deleting\n                    <jats:italic>Firre<\/jats:italic>\n                    weakens intra-megadomain interactions. However, deleting\n                    <jats:italic>Dxz4<\/jats:italic>\n                    and\/or\n                    <jats:italic>Firre<\/jats:italic>\n                    has no impact on Xi silencing and gene escape. Nor does it affect Xi nuclear localization, stability, or H3K27 methylation. Additionally, ectopic integration of\n                    <jats:italic>Dxz4<\/jats:italic>\n                    and\n                    <jats:italic>Xist<\/jats:italic>\n                    is not sufficient to form megadomains on autosomes. We conclude that\n                    <jats:italic>Dxz4<\/jats:italic>\n                    and megadomains are dispensable for Xi silencing and escape from X-inactivation.\n                  <\/jats:p>","DOI":"10.1038\/s41467-018-07446-w","type":"journal-article","created":{"date-parts":[[2018,11,21]],"date-time":"2018-11-21T08:20:12Z","timestamp":1542788412000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":62,"title":["Megadomains and superloops form dynamically but are dispensable for X-chromosome inactivation and gene escape"],"prefix":"10.1038","volume":"9","author":[{"given":"John E.","family":"Froberg","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan F.","family":"Pinter","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrea J.","family":"Kriz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teddy","family":"J\u00e9gu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7786-8850","authenticated-orcid":false,"given":"Jeannie T.","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2018,11,27]]},"reference":[{"key":"7446_CR1","doi-asserted-by":"publisher","first-page":"1665","DOI":"10.1016\/j.cell.2014.11.021","volume":"159","author":"SS Rao","year":"2014","unstructured":"Rao, S. 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