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Despite its critical role in survival within macrophages, only a few molecular targets of the MgtC protein have been identified. Here, we report that MgtC targets PhoR histidine kinase and activates phosphate transport independently of the available phosphate concentration. A single amino acid substitution in PhoR prevents its binding to MgtC, thus abrogating MgtC-mediated phosphate transport. Surprisingly, the removal of MgtC\u2019s effect on the ability to transport phosphate renders\n                    <jats:italic>Salmonella<\/jats:italic>\n                    hypervirulent and decreases a non-replicating population inside macrophages, indicating that MgtC-mediated phosphate transport is required for normal\n                    <jats:italic>Salmonella<\/jats:italic>\n                    pathogenesis. This provides an example of a virulence protein directly activating a pathogen\u2019s phosphate transport inside host.\n                  <\/jats:p>","DOI":"10.1038\/s41467-019-11318-2","type":"journal-article","created":{"date-parts":[[2019,7,25]],"date-time":"2019-07-25T06:03:26Z","timestamp":1564034606000},"update-policy":"https:\/\/doi.org\/10.1007\/springer_crossmark_policy","source":"Crossref","is-referenced-by-count":57,"title":["The Salmonella virulence protein MgtC promotes phosphate uptake inside macrophages"],"prefix":"10.1038","volume":"10","author":[{"given":"Soomin","family":"Choi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eunna","family":"Choi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Joon","family":"Cho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daesil","family":"Nam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jangwoo","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5078-1974","authenticated-orcid":false,"given":"Eun-Jin","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"297","published-online":{"date-parts":[[2019,7,25]]},"reference":[{"key":"11318_CR1","doi-asserted-by":"publisher","first-page":"198","DOI":"10.1016\/j.mib.2010.01.014","volume":"13","author":"YJ Hsieh","year":"2010","unstructured":"Hsieh, Y. 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